Backbone 1H, 13C, 15N and heavy atoms Chemical Shift Assignments for La3+ monosubstituted calbindin D9k
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 54.0 % (495 of 917) | 36.5 % (177 of 485) | 67.8 % (240 of 354) | 100.0 % (78 of 78) |
Backbone | 98.2 % (436 of 444) | 96.7 % (147 of 152) | 98.6 % (217 of 220) | 100.0 % (72 of 72) |
Sidechain | 23.2 % (126 of 543) | 9.0 % (30 of 333) | 44.1 % (90 of 204) | 100.0 % (6 of 6) |
Aromatic | 0.0 % (0 of 58) | 0.0 % (0 of 29) | 0.0 % (0 of 29) | |
Methyl | 3.9 % (3 of 76) | 2.6 % (1 of 38) | 5.3 % (2 of 38) |
1. Calcium Protein
KSPEELKGIF EKYAAKEGDP NQLSKEELKL LLQTEFPSLL KGMSTLDELF EELDKNGDGE VSFEEFQVLV KKISQTemperature 300 (±0.1) K, pH 6.0 (±0.01)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Calbindin D9k | [U-95% 13C; U-98% 15N] | 1 mM | |
2 | CALCIUM (II) ION | 1 mM | ||
3 | LANTHANUM (III) ION | 1 mM |