BMRB: 6715

Solution NMR Structure of the Membrane Protein Stannin

Authors

Buck-Koehntop, B.A., Mascioni, A., Buffy, J.J., Veglia, G.

Assembly

Stannin

Entity

1. Stannin (polymer, Thiol state: all free), 90 monomers, 9641.127 Da Detail

GSMSIMDHSP TTGVVTVIVI LIAIAALGAL ILGCWCYLRL QRISQSEDEE SIVGDGETKE PFLLVQYSAK GPCVERKAKL MTPNGPEVHG

Formula weight

9641.127 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 46.3 %, Completeness (bb): 80.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	46.3 % (466 of 1007)	38.2 % (198 of 518)	46.4 % (185 of 399)	92.2 % (83 of 90)
Backbone	80.2 % (425 of 530)	90.2 % (166 of 184)	67.8 % (177 of 261)	96.5 % (82 of 85)
Sidechain	8.8 % (49 of 558)	9.6 % (32 of 334)	7.3 % (16 of 219)	20.0 % (1 of 5)
Aromatic	0.0 % (0 of 46)	0.0 % (0 of 23)	0.0 % (0 of 22)	0.0 % (0 of 1)
Methyl	4.1 % (5 of 122)	4.9 % (3 of 61)	3.3 % (2 of 61)

1. Stannin

GSMSIMDHSP TTGVVTVIVI LIAIAALGAL ILGCWCYLRL QRISQSEDEE SIVGDGETKE PFLLVQYSAK GPCVERKAKL MTPNGPEVHG

Show sample condition

Sample

Pressure 1 atm, Temperature 333 K, pH 4.0

#	Name	Isotope labeling	Type	Concentration
1	Stannin	[U-15N]		0.5 ~ 0.7 mM
2	phosphate buffer			20 mM
3	d25-sodium dodecyl sulfate			600 mM
4	sodium azide			0.02 %
5	DTT			0.7 mM
6	H2O			90 %
7	D2O			10 %

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Protein Blocks Logo

Calculated from 25 models in PDB: 1ZZA, Strand ID: A Detail

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Release date

2007-01-28

Citation

Structure, dynamics, and membrane topology of stannin: a mediator of neuronal cell apoptosis induced by trimethyltin chloride
Buck-Koehntop, B.A., Mascioni, A., Buffy, J.J., Veglia, G.
J. Mol. Biol. (2005), 354, 652-665, PubMed 16246365 , DOI 10.1016/j.jmb.2005.09.038 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Stannin, : 1 : 5 : 6 entities Detail

Experiments performed 1 experiments Detail