BMRB: 6716

Solution structure of the helicase-interaction domain of the primase DnaG: a model for helicase activation

Authors

Syson, K., Thirlway, J., Hounslow, A.M., Soultanas, P., Waltho, J.P.

Assembly

DNA primase (E.C.2.7.7.-)

Entity

1. DNA primase (E.C.2.7.7.-) (polymer, Thiol state: not present), 146 monomers, 16820.35 Da Detail

MAKKLLPAFQ NAERLLLAHM MRSRDVALVV QERIGGRFNI EEHRALAAYI YAFYEEGHEA DPGALISRIP GELQPLASEL SLLLIADDVS EQELEDYIRH VLNRPKWLML KVKEQEKTEA ERRKDFLTAA RIAKEMIEMK KMLSSS

Formula weight

16820.35 Da

Source organism

Geobacillus stearothermophilus

Exptl. method

NMR

Refine. method

torsion angle dynamics followed by cartesian slow-cool annealing energy minimisation

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.9 %, Completeness: 82.5 %, Completeness (bb): 91.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	82.5 % (1449 of 1757)	78.8 % (727 of 923)	85.8 % (587 of 684)	90.0 % (135 of 150)
Backbone	91.7 % (794 of 866)	90.4 % (264 of 292)	92.4 % (400 of 433)	92.2 % (130 of 141)
Sidechain	75.8 % (782 of 1032)	73.4 % (463 of 631)	80.1 % (314 of 392)	55.6 % (5 of 9)
Aromatic	58.0 % (58 of 100)	58.0 % (29 of 50)	57.1 % (28 of 49)	100.0 % (1 of 1)
Methyl	93.3 % (166 of 178)	92.1 % (82 of 89)	94.4 % (84 of 89)

1. DNA primase

MAKKLLPAFQ NAERLLLAHM MRSRDVALVV QERIGGRFNI EEHRALAAYI YAFYEEGHEA DPGALISRIP GELQPLASEL SLLLIADDVS EQELEDYIRH VLNRPKWLML KVKEQEKTEA ERRKDFLTAA RIAKEMIEMK KMLSSS

Show sample condition

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	DNA primase	[U-15N]		1 mM
2	potassium phosphate			20 mM
3	sodium chloride			200 mM
4	H2O			90 %
5	D2O			10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
6	DNA primase	[U-13C; U-15N]		1 mM
7	potassium phosphate			20 mM
8	sodium chloride			200 mM
9	H2O			90 %
10	D2O			10 %

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LACS Plot; CA

Referencing offset: -0.36 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CB

Referencing offset: -0.36 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CO

Referencing offset: -0.22 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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Protein Blocks Logo

Calculated from 10 models in PDB: 1Z8S, Strand ID: A Detail

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Release date

2006-04-24

Citation

Solution structure of the helicase-interaction domain of the primase DnaG: a model for helicase activation
Syson, K., Thirlway, J., Hounslow, A.M., Soultanas, P., Waltho, J.P.
Structure (2005), 13, 609-616, PubMed 15837199 , DOI 10.1016/j.str.2005.01.022 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. DNA primase (E.C.2.7.7.-), : 1 : 1 : 3 : 4 entities Detail

Interaction partners 1. DNA primase (E.C.2.7.7.-), : 2 interactors Detail

Experiments performed 1 experiments Detail