BMRB: 6720

Solution Conformation of alpha-conotoxin PIA

Authors

Chi, S., Kim, D., Olivera, B.M., Mclntosh, J.M., Han, K.

Assembly

Alpha-conotoxin PIA

Entity

1. Alpha-conotoxin PIA (polymer, Thiol state: all disulfide bound), 19 monomers, 1984.285 Da Detail

RDPCCSNPVC TVHNPQICX

Formula weight

1984.285 Da

Entity Connection

disulfide 2 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS4:SG	1:CYS10:SG
2	disulfide	sing	1:CYS5:SG	1:CYS18:SG

Source organism

Conus purpurascens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.7 %, Completeness: 78.2 %, Completeness (bb): 97.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	78.2 % (79 of 101)	78.2 % (79 of 101)
Backbone	97.0 % (32 of 33)	97.0 % (32 of 33)
Sidechain	69.1 % (47 of 68)	69.1 % (47 of 68)
Aromatic	0.0 % (0 of 2)	0.0 % (0 of 2)
Methyl	85.7 % (6 of 7)	85.7 % (6 of 7)

1. Alpha-conotoxin PIA

RDPCCSNPVC TVHNPQICX

Show sample condition

Sample

Pressure 1 atm, Temperature 283 K, pH 3.92

#	Name	Isotope labeling	Type	Concentration
1	Alpha-conotoxin PIA			10 mM
2	H2O			90 %
3	D2O			10 %

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Calculated from 20 models in PDB: 1ZLC, Strand ID: A Detail

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Release date

2008-07-15

Citation

Solution structure of alpha-conotoxin PIA, a novel antagonist of alpha6 subunit containing nicotinic acetylcholine receptors
Chi, S., Lee, S., Kim, D., Kim, J., Olivera, B.M., Mclntosh, J.M., Han, K.
Biochem. Biophys. Res. Commun. (2005), 338, 1990-1997, PubMed 16289101 , DOI 10.1016/j.bbrc.2005.10.176 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Alpha-conotoxin PIA, : 1 : 1 : 19 entities Detail

Experiments performed 3 experiments Detail