BMRB: 6721

Solution structure of the first WW domain of FBP11

Authors

Kato, Y., Hino, Y., Tanokura, M.

Assembly

huntingtin-interacting protein HYPA/FBP11

Entity

1. huntingtin-interacting protein HYPA/FBP11 (polymer, Thiol state: not present), 30 monomers, 3607.716 Da Detail

GSWTEHKSPD GRTYYYNTET KQSTWEKPDD

Formula weight

3607.716 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.7 %, Completeness: 86.7 %, Completeness (bb): 79.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	86.7 % (306 of 353)	96.2 % (178 of 185)	72.1 % (98 of 136)	93.8 % (30 of 32)
Backbone	79.5 % (140 of 176)	93.3 % (56 of 60)	65.9 % (58 of 88)	92.9 % (26 of 28)
Sidechain	94.6 % (194 of 205)	97.6 % (122 of 125)	89.5 % (68 of 76)	100.0 % (4 of 4)
Aromatic	88.5 % (46 of 52)	100.0 % (26 of 26)	75.0 % (18 of 24)	100.0 % (2 of 2)
Methyl	100.0 % (10 of 10)	100.0 % (5 of 5)	100.0 % (5 of 5)

1. FBP11 WW1

GSWTEHKSPD GRTYYYNTET KQSTWEKPDD

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 5.0

#	Name	Isotope labeling	Type	Concentration
1	FBP11 WW1	[U-15N; U-13C]		1.5 mM
2	phosphate buffer			50 mM
3	NaCl			50 mM
4	H2O			90 %
5	D2O			10 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 1ZR7, Strand ID: A Detail

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Release date

2006-04-23

Citation

Solution structure and binding specificity of FBP11/HYPA WW domain as Group-II/III
Kato, Y., Hino, Y., Nagata, K., Tanokura, M.
Proteins (2006), 63, 227-234, PubMed 16463264 , DOI 10.1002/prot.20880 , Abstract

Related entities 1. huntingtin-interacting protein HYPA/FBP11, : 1 : 1 : 8 : 324 entities Detail

Interaction partners 1. huntingtin-interacting protein HYPA/FBP11, : 96 interactors Detail

Experiments performed 2 experiments Detail