BMRB: 6727

1H, 13C and 15N resonance assignments of telomeric repeat-binding domain of Arabidopsis thaliana

Authors

Cheng, Y., Sue, S., Hsaio, H., Huang, T.

Assembly

telomeric repeat-binding domain of Arabidopsis thaliana

Entity

1. telomeric repeat-binding domain of Arabidopsis thaliana (polymer, Thiol state: not present), 105 monomers, 12300.82 Da Detail

GSHMLEDPQR RIRRPFSVAE VEALVQAVEK LGTGRWRDVK LCAFEDADHR TYVDLKDKWK TLVHTAKISP QQRRGEPVPQ ELLNRVLNAH GYWTQQQMQQ LQQNV

Formula weight

12300.82 Da

Source organism

Arabidopsis thaliana

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.3 %, Completeness: 80.1 %, Completeness (bb): 91.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	80.1 % (1021 of 1274)	77.7 % (523 of 673)	83.0 % (401 of 483)	82.2 % (97 of 118)
Backbone	91.6 % (568 of 620)	89.0 % (187 of 210)	92.6 % (287 of 310)	94.0 % (94 of 100)
Sidechain	72.1 % (544 of 754)	72.6 % (336 of 463)	75.1 % (205 of 273)	16.7 % (3 of 18)
Aromatic	58.0 % (51 of 88)	63.6 % (28 of 44)	48.8 % (20 of 41)	100.0 % (3 of 3)
Methyl	85.7 % (96 of 112)	87.5 % (49 of 56)	83.9 % (47 of 56)

1. telomeric repeat-binding domain of Arabidopsis thaliana

GSHMLEDPQR RIRRPFSVAE VEALVQAVEK LGTGRWRDVK LCAFEDADHR TYVDLKDKWK TLVHTAKISP QQRRGEPVPQ ELLNRVLNAH GYWTQQQMQQ LQQNV

Show sample condition

Sample

Temperature 298 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	AtTRP1d	[U-13C; U-15N]		1 mM

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LACS Plot; CA

Referencing offset: 0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.45 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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Protein Blocks Logo

Calculated from 15 models in PDB: 2AJE, Strand ID: A Detail

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Release date

2006-02-12

Citation

Solution structure of the Arabidopsis thaliana telomeric repeat-binding protein DNA binding domain: a new fold with an additional C-terminal helix
Sue, S., Hsaio, H., Chung, C.P., Cheng, Y., Hsueh, K., Chen, C., Ho, C., Huang, T.
J. Mol. Biol. (2006), 356, 72-85, PubMed 16337232 , DOI 10.1016/j.jmb.2005.11.009 , Abstract

Related entities 1. telomeric repeat-binding domain of Arabidopsis thaliana, : 1 : 2 : 32 entities Detail

Interaction partners 1. telomeric repeat-binding domain of Arabidopsis thaliana, : 5 interactors Detail

Experiments performed 11 experiments Detail