BMRB: 6729

1H Chemical Shift Assignments for Diapause-Specific Peptide from the Leaf Beetle

Authors

Kouno, T., Mizuguchi, M., Unoki, K., Suzuki, K., Kawano, K.

Assembly

DSP monomer

Entity

1. DSP monomer (polymer, Thiol state: all disulfide bound), 41 monomers, 4473.092 Da Detail

AVRIGPCDQV CPRIVPERHE CCRAHGRSGY AYCSGGGMYC N

Formula weight

4473.092 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS7:SG	1:CYS21:SG
2	disulfide	sing	1:CYS11:SG	1:CYS33:SG
3	disulfide	sing	1:CYS22:SG	1:CYS40:SG

Source organism

Gastrophysa atrocyanea

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.1 %, Completeness: 93.0 %, Completeness (bb): 92.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	93.0 % (211 of 227)	93.0 % (211 of 227)
Backbone	92.9 % (79 of 85)	92.9 % (79 of 85)
Sidechain	93.0 % (132 of 142)	93.0 % (132 of 142)
Aromatic	75.0 % (12 of 16)	75.0 % (12 of 16)
Methyl	100.0 % (13 of 13)	100.0 % (13 of 13)

1. DSP

AVRIGPCDQV CPRIVPERHE CCRAHGRSGY AYCSGGGMYC N

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	DSP			0.15 mM
2	sodium phosphate			10 mM
3	NaN3			0.2 mM
4	DSS			0.05 mM
5	D2O			5 %

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Protein Blocks Logo

Calculated from 25 models in PDB: 2E2F, Strand ID: A Detail

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Release date

2006-04-19

Citation

The structure of a novel insect peptide explains its Ca2+ channel blocking and antifungal activities
Kouno, T., Mizuguchi, M., Unoki, K., Tanaka, H., Yang, P., Koichi, S., Kawano, K.
Biochemistry (2007), 46, 13733-13741, PubMed 17994764 , DOI 10.1021/bi701319t , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. DSP monomer, : 1 : 1 : 3 entities Detail

Experiments performed 3 experiments Detail