BMRB: 6730

Chemical shift assignments for Filamin a domain 17

Authors

Heikkinen, O.K., Kilpelainen, I.A., Permi, P.

Assembly

Human Filamin A domain 17

Entity

1. Human Filamin A domain 17 (polymer, Thiol state: all free), 98 monomers, 10067.08 Da Detail

GAMVVNCGHV TAYGPGLTHG VVNKPATFTV NTKDAGEGGL SLAIEGPSKA EISCTDNQDG TCSVSYLPVL PGDYSILVKY NEQHVPGSPF TARVTGDD

Formula weight

10067.08 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

The structure calculations were made with the program CYANA 2.0 using the automated NOE assignment and structure calculation algorithm. The structure refinement of the 20 final CYANA structures was made with AMBER 8.0 program using the generalized Born continuum solvent model.

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 92.4 %, Completeness (bb): 95.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.4 % (948 of 1026)	92.3 % (481 of 521)	91.4 % (372 of 407)	96.9 % (95 of 98)
Backbone	95.3 % (547 of 574)	97.0 % (196 of 202)	93.2 % (262 of 281)	97.8 % (89 of 91)
Sidechain	90.3 % (485 of 537)	89.3 % (285 of 319)	91.9 % (194 of 211)	85.7 % (6 of 7)
Aromatic	62.5 % (40 of 64)	62.5 % (20 of 32)	62.5 % (20 of 32)
Methyl	95.5 % (107 of 112)	96.4 % (54 of 56)	94.6 % (53 of 56)

1. Filamin A domain 17

GAMVVNCGHV TAYGPGLTHG VVNKPATFTV NTKDAGEGGL SLAIEGPSKA EISCTDNQDG TCSVSYLPVL PGDYSILVKY NEQHVPGSPF TARVTGDD

Show sample condition

Sample

Temperature 293 (±2) K, pH 6.70 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Filamin A domain 17	[U-13C; U-15N]	protein	0.3 mM
2	Sodium phosphate		buffer	50 mM
3	Dithiothreitol		reducing agent	10 mM

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LACS Plot; CA

Referencing offset: 0.75 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	Water	protons	4.83 ppm	internal	direct	1.0

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LACS Plot; CB

Referencing offset: 0.75 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	Water	protons	4.83 ppm	internal	direct	1.0

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LACS Plot; HA

Referencing offset: -0.18 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	Water	protons	4.83 ppm	internal	direct	1.0

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LACS Plot; CO

Referencing offset: 44.94 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	Water	protons	4.83 ppm	internal	direct	1.0

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Protein Blocks Logo

Calculated from 20 models in PDB: 2AAV, Strand ID: A Detail

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Release date

2007-02-04

Citation

The structure of the GPIb-filamin A complex
Nakamura, F., Pudas, R., Heikkinen, O.K., Permi, P., Kilpelainen, I.A., Munday, A.D., Hartwig, J.H., Stossel, T.P., Ylanne, J.
Blood (2006), 107, 1925-1932, PubMed 16293600 , DOI 10.1182/blood-2005-10-3964 , Abstract

Related entities 1. Human Filamin A domain 17, : 1 : 3 : 451 entities Detail

Interaction partners 1. Human Filamin A domain 17, : 141 interactors Detail