BMRB: 6731

Backbone 1H, 13C, and 15N Chemical Shift Assignments for BOFC

Authors

Patterson, H.M., Brannigan, J.A., Cutting, S.M., Wilson, K.S., Wilkinson, A.J., Ab, E., Diercks, T., Folkers, G., de Jong, R., Truffault, V.

Assembly

BofC

Entity

1. BofC (polymer, Thiol state: not present), 140 monomers, 16173.06 Da Detail

AEVEHYEPLQ VHVQLEKVYL DGDVSIEHKH EKVFSMDDFW AAYAGWTLVE QKKGYVLFRK QMDDISPLSK VNGYIGVSDN GVISTFHGRP EPASEPIQSF FQIDLERLES HMQKNLLKGI PFRTKAEFED VIEHMKTYSG

Formula weight

16173.06 Da

Source organism

Bacillus subtilis

Exptl. method

NMR

Refine. method

CANDID AND CNS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 91.9 %, Completeness (bb): 95.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.9 % (1552 of 1688)	91.5 % (804 of 879)	92.3 % (612 of 663)	93.2 % (136 of 146)
Backbone	95.4 % (790 of 828)	94.3 % (267 of 283)	96.8 % (398 of 411)	93.3 % (125 of 134)
Sidechain	89.7 % (889 of 991)	90.1 % (537 of 596)	89.0 % (341 of 383)	91.7 % (11 of 12)
Aromatic	65.6 % (118 of 180)	68.9 % (62 of 90)	61.4 % (54 of 88)	100.0 % (2 of 2)
Methyl	97.9 % (137 of 140)	97.1 % (68 of 70)	98.6 % (69 of 70)

1. BofC

AEVEHYEPLQ VHVQLEKVYL DGDVSIEHKH EKVFSMDDFW AAYAGWTLVE QKKGYVLFRK QMDDISPLSK VNGYIGVSDN GVISTFHGRP EPASEPIQSF FQIDLERLES HMQKNLLKGI PFRTKAEFED VIEHMKTYSG

Show sample condition

Sample

Pressure 1013 hPa, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	BofC	[U-15N; U-13C]		0.5 mM
2	H2O			90 %
3	D2O			10 %
4	NaPO4			20 mM
5	EDTA			1 mM

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LACS Plot; CA

Referencing offset: 0.15 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.15 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.08 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 25 models in PDB: 2BW2, Strand ID: A Detail

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Release date

2007-01-28

Citation

The structure of bypass of forespore C, an intercompartmental signaling factor during sporulation in Bacillus
Patterson, H.M., Brannigan, J.A., Cutting, S.M., Wilson, K.S., Wilkinson, A.J., Ab, E., Diercks, T., Folkers, G., de Jong, R., Truffault, V., Kaptein, R.
J. Biol. Chem. (2005), 280, 36214-36220, PubMed 16049010 , DOI 10.1074/jbc.M506910200 , Abstract

Related entities 1. BofC, : 1 : 1 entities Detail

Experiments performed 1 experiments Detail