BMRB: 6738

Solution structure of Sep15 from Drosophila melanogaster

Authors

Ferguson, A.D., Labunskyy, V.M., Fomenko, D.E., Arac, D., Chelliah, Y., Amezcua, C.A., Rizo, J., Gladyshev, V.N., Deisenhofer, J.

Assembly

Selenoprotein Sep15

Entity

1. Selenoprotein Sep15 (polymer, Thiol state: all disulfide bound), 126 monomers, 14297.80 Da Detail

MASHHHHHHL DQQPAAQRTY AKAILEVCTC KFRAYPQIQA FIQSGRPAKF PNLQIKYVRG LDPVVKLLDA SGKVQETLSI TKWNTDTVEE FFETHLAKDG AGKNSYSVVE DADGDDDEDY LRTNRI

Formula weight

14297.8 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS28:SG	1:CYS30:SG

Source organism

Drosophila melanogaster

Exptl. method

NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.2 %, Completeness: 90.8 %, Completeness (bb): 91.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.8 % (1330 of 1465)	91.1 % (692 of 760)	89.7 % (512 of 571)	94.0 % (126 of 134)
Backbone	91.8 % (685 of 746)	93.3 % (236 of 253)	90.3 % (336 of 372)	93.4 % (113 of 121)
Sidechain	90.3 % (758 of 839)	90.1 % (457 of 507)	90.3 % (288 of 319)	100.0 % (13 of 13)
Aromatic	72.3 % (94 of 130)	72.3 % (47 of 65)	71.9 % (46 of 64)	100.0 % (1 of 1)
Methyl	95.5 % (126 of 132)	95.5 % (63 of 66)	95.5 % (63 of 66)

1. Selenoprotein Sep15

MASHHHHHHL DQQPAAQRTY AKAILEVCTC KFRAYPQIQA FIQSGRPAKF PNLQIKYVRG LDPVVKLLDA SGKVQETLSI TKWNTDTVEE FFETHLAKDG AGKNSYSVVE DADGDDDEDY LRTNRI

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	Selenoprotein Sep15	[U-15N; U-13C]		1 mM
2	phosphate buffer			50 mM
3	H2O			90 %
4	D2O			10 %

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LACS Plot; CA

Referencing offset: -0.09 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	null	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.09 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	null	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	null	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.27 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	null	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2A4H, Strand ID: A Detail

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Release date

2005-12-27

Citation

NMR structures of the selenoproteins Sep15 and SelM reveal redox activity of a new thioredoxin-like family
Ferguson, A.D., Labunskyy, V.M., Fomenko, D.E., Arac, D., Chelliah, Y., Amezcua, C.A., Rizo, J., Gladyshev, V.N., Deisenhofer, J.
J. Biol. Chem. (2006), 281, 3536-3543, PubMed 16319061 , DOI 10.1074/jbc.M511386200 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

Related entities 1. Selenoprotein Sep15, : 1 : 1 entities Detail

Experiments performed 4 experiments Detail