BMRB: 6739

Solution structure of SelM from Mus musculus

Authors

Ferguson, A.D., Labunskyy, V.M., Fomenko, D.E., Arac, D., Chelliah, Y., Amezcua, C.A., Rizo, J., Gladyshev, V.N., Deisenhofer, J.

Assembly

Selenoprotein M

Entity

1. Selenoprotein M (polymer), 129 monomers, 15038.79 Da Detail

MTNYRPDWNR LRGLARGRVE TCGGCQLNRL KEVKAFVTED IQLYHNLVMK HLPGADPELV LLSRNYQELE RIPLSQMTRD EINALVQELG FYRKSAPEAQ VPPEYLWAPA KPPEEASEHD DLEHHHHHH

Formula weight

15038.79 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS22:SG	1:CYS25:SG

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

Distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.1 %, Completeness: 90.8 %, Completeness (bb): 89.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.8 % (1394 of 1536)	91.0 % (735 of 808)	90.1 % (536 of 595)	92.5 % (123 of 133)
Backbone	89.9 % (678 of 754)	90.2 % (229 of 254)	89.2 % (340 of 381)	91.6 % (109 of 119)
Sidechain	91.7 % (830 of 905)	91.3 % (506 of 554)	92.0 % (310 of 337)	100.0 % (14 of 14)
Aromatic	76.7 % (92 of 120)	76.7 % (46 of 60)	75.9 % (44 of 58)	100.0 % (2 of 2)
Methyl	97.7 % (127 of 130)	95.4 % (62 of 65)	100.0 % (65 of 65)

1. Selenoprotein M

MTNYRPDWNR LRGLARGRVE TCGGCQLNRL KEVKAFVTED IQLYHNLVMK HLPGADPELV LLSRNYQELE RIPLSQMTRD EINALVQELG FYRKSAPEAQ VPPEYLWAPA KPPEEASEHD DLEHHHHHH

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	Selenoprotein M	[U-15N; U-13C]		1 mM
2	phosphate buffer			50 mM
3	H2O			90 %
4	D2O			10 %

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LACS Plot; CA

Referencing offset: -0.12 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	null	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.12 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	null	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	null	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.23 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	null	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2A2P, Strand ID: A Detail

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Release date

2005-12-27

Citation

NMR structures of the selenoproteins Sep15 and SelM reveal redox activity of a new thioredoxin-like family
Ferguson, A.D., Labunskyy, V.M., Fomenko, D.E., Arac, D., Chelliah, Y., Amezcua, C.A., Rizo, J., Gladyshev, V.N., Deisenhofer, J.
J. Biol. Chem. (2006), 281, 3536-3543, PubMed 16319061 , DOI 10.1074/jbc.M511386200 , Abstract

Related entities 1. Selenoprotein M, : 1 : 1 : 6 entities Detail

Experiments performed 4 experiments Detail