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Found 1 Document (1.733 seconds)

BMRB: 6740

2A05

NMR solution structure of the Crisp domain of Tpx-1

Authors

Gibbs, G.M., Scanlon, M.J., Swarbrick, J.D., Curtis, S., Dulhunty, A., O'Bryan, M.K.

Assembly

Cysteine-rich secretory protein-2

Entity

1. Cysteine-rich secretory protein-2 (polymer, Thiol state: all disulfide bound), 57 monomers, 6114.873 Da Detail

GSCASCPNNC ENGLCTNSCD FEDLLSNCES LKTSAGCKHE LLKTKCQATC LCEDKIH

Formula weight

6114.873 Da

Entity Connection

disulfide 5 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS3:SG	1:CYS10:SG
2	disulfide	sing	1:CYS6:SG	1:CYS15:SG
3	disulfide	sing	1:CYS19:SG	1:CYS52:SG
4	disulfide	sing	1:CYS28:SG	1:CYS46:SG
5	disulfide	sing	1:CYS37:SG	1:CYS50:SG

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

Automated Resonance assignment using Torsion Angle Dynamics (CANDID), Further refinement with CA and CB chem shifts and database potentials

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.2 %, Completeness: 66.6 %, Completeness (bb): 71.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	66.6 % (401 of 602)	83.7 % (261 of 312)	36.0 % (82 of 228)	93.5 % (58 of 62)
Backbone	71.8 % (244 of 340)	94.8 % (110 of 116)	48.8 % (82 of 168)	92.9 % (52 of 56)
Sidechain	62.7 % (198 of 316)	77.0 % (151 of 196)	36.0 % (41 of 114)	100.0 % (6 of 6)
Aromatic	0.0 % (0 of 18)	0.0 % (0 of 9)	0.0 % (0 of 9)
Methyl	47.8 % (22 of 46)	82.6 % (19 of 23)	13.0 % (3 of 23)

1. Cysteine-rich secretory protein-2

GSCASCPNNC ENGLCTNSCD FEDLLSNCES LKTSAGCKHE LLKTKCQATC LCEDKIH

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 5.8

#	Name	Isotope labeling	Concentration
1	Cysteine-rich secretory protein-2	[U-13C; U-15]	1 mM
2	H2O		90 %
3	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	null	0.0
¹H	DSS	methyl protons	null	null	1.0
¹⁵N	DSS	methyl protons	null	null	0.1013291

Referencing offset: 0.19 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	null	0.0
¹H	DSS	methyl protons	null	null	1.0
¹⁵N	DSS	methyl protons	null	null	0.1013291

Referencing offset: 0.19 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	null	0.0
¹H	DSS	methyl protons	null	null	1.0
¹⁵N	DSS	methyl protons	null	null	0.1013291

Referencing offset: 0.05 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 23 models in PDB: 2A05, Strand ID: A Detail

Release date

2005-08-10

Citation

The cysteine-rich secretory protein domain of Tpx-1 is related to ion channel toxins and regulates ryanodine receptor Ca2+ signaling
Gibbs, G.M., Scanlon, M.J., Swarbrick, J.D., Curtis, S., Gallant, E., Dulhunty, A.F., O'Bryan, M.K.
J. Biol. Chem. (2006), 281, 4156-4163, PubMed 16339766 , DOI 10.1074/jbc.M506849200 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P16563 released on 1990-08-01
Title CRIS2_MOUSE Entity Cysteine-rich secretory protein 2

Related entities 1. Cysteine-rich secretory protein-2, : 1 : 2 : 72 entities Detail

Experiments performed 5 experiments Detail

1 3D_15N-separated_NOESY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 5.8

#	Name	Isotope labeling	Concentration
1	Cysteine-rich secretory protein-2	[U-13C; U-15]	1 mM
2	H2O		90 %
3	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 5.8

#	Name	Concentration
4	Cysteine-rich secretory protein-2	1 mM
5	H2O	90 %
6	D2O	10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 5.8

#	Name	Isotope labeling	Type	Concentration
7	Cysteine-rich secretory protein-2	[U-13C; U-15]		1 mM
8	D2O			100 %

2 3D_15N-separated TOCSY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 5.8

#	Name	Isotope labeling	Concentration
1	Cysteine-rich secretory protein-2	[U-13C; U-15]	1 mM
2	H2O		90 %
3	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 5.8

#	Name	Concentration
4	Cysteine-rich secretory protein-2	1 mM
5	H2O	90 %
6	D2O	10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 5.8

#	Name	Isotope labeling	Type	Concentration
7	Cysteine-rich secretory protein-2	[U-13C; U-15]		1 mM
8	D2O			100 %

3 2D NOESY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 5.8

#	Name	Isotope labeling	Concentration
1	Cysteine-rich secretory protein-2	[U-13C; U-15]	1 mM
2	H2O		90 %
3	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 5.8

#	Name	Concentration
4	Cysteine-rich secretory protein-2	1 mM
5	H2O	90 %
6	D2O	10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 5.8

#	Name	Isotope labeling	Type	Concentration
7	Cysteine-rich secretory protein-2	[U-13C; U-15]		1 mM
8	D2O			100 %

4 2D TOCSY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 5.8

#	Name	Isotope labeling	Concentration
1	Cysteine-rich secretory protein-2	[U-13C; U-15]	1 mM
2	H2O		90 %
3	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 5.8

#	Name	Concentration
4	Cysteine-rich secretory protein-2	1 mM
5	H2O	90 %
6	D2O	10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 5.8

#	Name	Isotope labeling	Type	Concentration
7	Cysteine-rich secretory protein-2	[U-13C; U-15]		1 mM
8	D2O			100 %

5 DQF-COSY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 5.8

#	Name	Isotope labeling	Concentration
1	Cysteine-rich secretory protein-2	[U-13C; U-15]	1 mM
2	H2O		90 %
3	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 5.8

#	Name	Concentration
4	Cysteine-rich secretory protein-2	1 mM
5	H2O	90 %
6	D2O	10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 5.8

#	Name	Isotope labeling	Type	Concentration
7	Cysteine-rich secretory protein-2	[U-13C; U-15]		1 mM
8	D2O			100 %

nullKeywords 2 domains, 3 Alpha Helices, 5 Disulphide bonds

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Found 1 Document (1.733 seconds)

BMRB: 6740

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Cysteine-rich secretory protein-2, : 1 : 2 : 72 entities Detail

Experiments performed 5 experiments Detail

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