BMRB: 6741

NMR Structure of Mini-B, an N-terminal - C-terminal construct from Surfactant Protein B (SP-B), in Sodium Dodecyl Sulfate (SDS) and Hexafluoroisopropanol (HFIP)

Authors

Booth, V., Sarker, M., Keough, K.M.W., Waring, A.J., Walther, F.J.

Assembly

Pulmonary surfactant-associated protein B

Entity

1. Pulmonary surfactant-associated protein B (polymer, Thiol state: all free), 34 monomers, 3926.964 Da Detail

CWLCRALIKR IQAMIPKGGR MLPQLVCRLV LRCS

Formula weight

3926.964 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 100.0 %, Completeness: 98.2 %, Completeness (bb): 100.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	98.2 % (220 of 224)	98.2 % (220 of 224)
Backbone	100.0 % (68 of 68)	100.0 % (68 of 68)
Sidechain	97.4 % (152 of 156)	97.4 % (152 of 156)
Aromatic	100.0 % (6 of 6)	100.0 % (6 of 6)
Methyl	100.0 % (24 of 24)	100.0 % (24 of 24)

1. Pulmonary surfactant-associated protein B

CWLCRALIKR IQAMIPKGGR MLPQLVCRLV LRCS

Show sample condition

Sample

Pressure 1 atm, Temperature 318 K, pH 5

#	Name	Isotope labeling	Type	Concentration
1	Pulmonary surfactant-associated protein B	[15N]-Leu(3,7,22,25,29,31), Ala(6,13), and Gly(18)		1.5 mM
2	SDS			150 mM
3	DSS			0.4 mM
4	NaN3			0.2 mM
5	H2O			90 %
6	D2O			10 %

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Chem. Shift Complete2

Sequence coverage: 100.0 %, Completeness: 98.2 %, Completeness (bb): 100.0 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H
All	98.2 % (440 of 448)	98.2 % (440 of 448)
Backbone	100.0 % (136 of 136)	100.0 % (136 of 136)
Sidechain	97.4 % (304 of 312)	97.4 % (304 of 312)
Aromatic	100.0 % (12 of 12)	100.0 % (12 of 12)
Methyl	100.0 % (48 of 48)	100.0 % (48 of 48)

1. Pulmonary surfactant-associated protein B

CWLCRALIKR IQAMIPKGGR MLPQLVCRLV LRCS

Show sample condition

Sample

Pressure 1 atm, Temperature 288 K, pH 5

#	Name	Isotope labeling	Type	Concentration
7	Pulmonary surfactant-associated protein B	[15N]-Leu(3,7,22,25,29,31), Ala(6,13), and Gly(18)		1.5 mM
8	HFIP			40 mM
9	DSS			0.4 mM
10	NaN3			0.2 mM
11	H2O			90 %
12	D2O			10 %

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Protein Blocks Logo

Calculated from 15 models in PDB: 2JOU, Strand ID: A Detail

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Release date

2007-03-26

Citation

Structure of mini-B, a functional fragment of surfactant protein B, in detergent micelles
Sarker, M., Waring, A.J., Walther, F.J., Keough, K.M.W., Booth, V.
Biochemistry (2007), 46, 11047-11056, PubMed 17845058 , DOI 10.1021/bi7011756 , Abstract

Related entities 1. Pulmonary surfactant-associated protein B, : 1 : 2 : 11 entities Detail

Interaction partners 1. Pulmonary surfactant-associated protein B, : 4 interactors Detail

Experiments performed 4 experiments Detail