BMRBj - BMREntryMaster

Found 1 Document (1.508 seconds)

BMRB: 6746

2AKK

Assignment of phnA-like protein rp4479

Authors

Wu, B., Yee, A., Ramelot, T., Semesi, A., Lemak, A., Kennedy, M., Arrowsmith, C.H.

Assembly

phnA-like protein rp4479

Entity

1. phnA-like protein rp4479 (polymer, Thiol state: not present), 74 monomers, 8077.278 Da Detail

MSIEVRDCNG ALLADGDNVS LIKDLKLKGS STVLKRGTMI RGIRLTDSED EIEGRTDKIK GLVLRTEFLK KAGS

Formula weight

8077.278 Da

Source organism

Thermoplasma acidophilum

Exptl. method

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.6 %, Completeness: 96.3 %, Completeness (bb): 97.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.3 % (810 of 841)	95.9 % (422 of 440)	96.6 % (314 of 325)	97.4 % (74 of 76)
Backbone	97.1 % (431 of 444)	96.8 % (151 of 156)	97.2 % (208 of 214)	97.3 % (72 of 74)
Sidechain	95.9 % (444 of 463)	95.4 % (271 of 284)	96.6 % (171 of 177)	100.0 % (2 of 2)
Aromatic	30.0 % (3 of 10)	40.0 % (2 of 5)	20.0 % (1 of 5)
Methyl	100.0 % (96 of 96)	100.0 % (48 of 48)	100.0 % (48 of 48)

1. phnA-like protein rp4479

MSIEVRDCNG ALLADGDNVS LIKDLKLKGS STVLKRGTMI RGIRLTDSED EIEGRTDKIK GLVLRTEFLK KAGS

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	phnA-like protein rp4479	[U-13C; U-15N]		1 mM
2	NaCl			450 mM
3	Phosphate			25 mM

LACS Plot; CA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: -0.11 ppm, Outliers: 2 Detail

LACS Plot; CO

Referencing offset: 0.51 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2AKK, Strand ID: A Detail

Release date

2006-02-22

Citation

Solution structure of phnA-like protein rp4479 from Rhodopseudomonas palustris
Wu, B., Yee, A., Ramelot, T., Semesi, A., Lemak, A., Kennedy, M., Arrowsmith, C.H.

Related entities 1. phnA-like protein rp4479, : 1 : 13 entities Detail

Experiments performed 11 experiments Detail

1 15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	phnA-like protein rp4479	[U-13C; U-15N]		1 mM
2	NaCl			450 mM
3	Phosphate			25 mM

2 HNCO

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	phnA-like protein rp4479	[U-13C; U-15N]		1 mM
2	NaCl			450 mM
3	Phosphate			25 mM

3 CBCAcoNH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	phnA-like protein rp4479	[U-13C; U-15N]		1 mM
2	NaCl			450 mM
3	Phosphate			25 mM

4 HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	phnA-like protein rp4479	[U-13C; U-15N]		1 mM
2	NaCl			450 mM
3	Phosphate			25 mM

5 CCTOCSY_NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	phnA-like protein rp4479	[U-13C; U-15N]		1 mM
2	NaCl			450 mM
3	Phosphate			25 mM

6 HCCTOCSY_NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	phnA-like protein rp4479	[U-13C; U-15N]		1 mM
2	NaCl			450 mM
3	Phosphate			25 mM

7 13C HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	phnA-like protein rp4479	[U-13C; U-15N]		1 mM
2	NaCl			450 mM
3	Phosphate			25 mM

8 HCCHTOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	phnA-like protein rp4479	[U-13C; U-15N]		1 mM
2	NaCl			450 mM
3	Phosphate			25 mM

9 15N NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	phnA-like protein rp4479	[U-13C; U-15N]		1 mM
2	NaCl			450 mM
3	Phosphate			25 mM

10 13C NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	phnA-like protein rp4479	[U-13C; U-15N]		1 mM
2	NaCl			450 mM
3	Phosphate			25 mM

11 13,13C 4d CCNOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	phnA-like protein rp4479	[U-13C; U-15N]		1 mM
2	NaCl			450 mM
3	Phosphate			25 mM

nullKeywords phnA-like protein rp4479