BMRB: 6748

NMR assignments of the Ki67FHA/hNIFK(226-269)3P complex

Authors

Byeon, I.L., Gronenborn, A.M., Li, H., Tsai, M.

Assembly

The Ki67FHA/hNIFK(226-269)3P complex

Entity

1. Ki67FHA (polymer, Thiol state: all free), 120 monomers, 13804.58 Da Detail

MWPTRRLVTI KRSGVDGPHF PLSLSTCLFG RGIECDIRIQ LPVVSKQHCK IEIHEQEAIL HNFSSTNPTQ VNGSVIDEPV RLKHGDVITI IDRSFRYENE SLQNGRKSTE FPRKIREQEP

2. hNIFK (polymer, Thiol state: all free), 44 monomers, 4951.453 Da Detail

KTVDSQGPTP VCTPTFLERR KSQVAELNDD DKDDEIVFKQ PISC

Total weight

18756.033 Da

Max. entity weight

13804.58 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.4 %, Completeness: 83.2 %, Completeness (bb): 83.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	83.2 % (1614 of 1940)	87.2 % (892 of 1023)	74.6 % (559 of 749)	97.0 % (163 of 168)
Backbone	83.7 % (804 of 960)	95.4 % (309 of 324)	71.5 % (346 of 484)	98.0 % (149 of 152)
Sidechain	84.8 % (963 of 1136)	83.4 % (583 of 699)	86.9 % (366 of 421)	87.5 % (14 of 16)
Aromatic	85.5 % (94 of 110)	94.5 % (52 of 55)	75.9 % (41 of 54)	100.0 % (1 of 1)
Methyl	97.1 % (165 of 170)	97.6 % (83 of 85)	96.5 % (82 of 85)

1. Ki67FHA

MWPTRRLVTI KRSGVDGPHF PLSLSTCLFG RGIECDIRIQ LPVVSKQHCK IEIHEQEAIL HNFSSTNPTQ VNGSVIDEPV RLKHGDVITI IDRSFRYENE SLQNGRKSTE FPRKIREQEP

2. hNIFK

KTVDSQGPTP VCTPTFLERR KSQVAELNDD DKDDEIVFKQ PISC

Show sample condition

Sample

Temperature 290 (±0) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ki67FHA	[U-13C; U-15N]		1 mM
2	hNIFK			1 mM

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LACS Plot; CA

Referencing offset: 0.11 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.11 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Release date

2007-01-28

Citation

Sequential phosphorylation and multisite interactions characterize specific target recognition by the FHA domain of Ki67
Byeon, I.L., Li, H., Song, H., Gronenborn, A.M., Tsai, M.
Nat. Struct. Mol. Biol. (2005), 12, 987-993, PubMed 16244663 , DOI 10.1038/nsmb1008 , Abstract

Related entities 1. Ki67FHA, : 1 : 2 entities Detail

Related entities 2. hNIFK, : 1 : 2 : 11 entities Detail

Interaction partners 1. Ki67FHA, : 40 : 1 interactors Detail

Interaction partners 2. hNIFK, : 27 : 1 interactors Detail