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BMRB: 6749

2CZY

Solution structure of the NRSF/REST-mSin3B PAH1 complex

Authors

Nishimura, Y., Nomura, M.

Assembly

mSin3B PAH1 and NRSF/REST

Entity

1. mSin3B PAH1 (polymer, Thiol state: not present), 80 monomers, 9172.432 Da Detail

EKLPVHVEDA LTYLDQVKIR FGSDPATYNG FLEIMKEFKS QSIDTPGVIR RVSQLFHEHP DLIVGFNAFL PLGYRIDIPK

2. NRSF/REST (polymer, Thiol state: not present), 15 monomers, 1540.821 Da Detail

APQLIMLANV ALTGE

Total weight

10713.253 Da

Max. entity weight

9172.432 Da

Source organism

Mus musculus , Homo sapiens

Exptl. method

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 84.5 %, Completeness (bb): 88.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.5 % (963 of 1139)	88.2 % (521 of 591)	80.4 % (364 of 453)	82.1 % (78 of 95)
Backbone	88.8 % (494 of 556)	98.4 % (186 of 189)	83.9 % (234 of 279)	84.1 % (74 of 88)
Sidechain	81.0 % (544 of 672)	83.3 % (335 of 402)	77.9 % (205 of 263)	57.1 % (4 of 7)
Aromatic	58.3 % (56 of 96)	62.5 % (30 of 48)	54.2 % (26 of 48)
Methyl	88.7 % (110 of 124)	100.0 % (62 of 62)	77.4 % (48 of 62)

1. mSin3B PAH1

EKLPVHVEDA LTYLDQVKIR FGSDPATYNG FLEIMKEFKS QSIDTPGVIR RVSQLFHEHP DLIVGFNAFL PLGYRIDIPK

2. NRSF/REST

APQLIMLANV ALTGE

Show sample condition

Sample

Temperature 293 (±0.2) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	mSin3B PAH1	[U-13C; U-15N]		0.8 mM
2	NRSF/REST			0.8 mM
3	potassium phosphate buffer			20 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 2CZY, Strand ID: A, B Detail

Release date

2005-12-12

Citation

The Neural Repressor NRSF/REST Binds the PAH1 Domain of the Sin3 Corepressor by Using its Distinct Short Hydrophobic Helix
Nomura, M., Uda-Tochio, H., Murai, K., Mori, N., Nishimura, Y.
J. Mol. Biol. (2005), 354, 903-915, PubMed , DOI:

Related entities 1. mSin3B PAH1, : 5 : 152 entities Detail

Related entities 2. NRSF/REST, : 1 : 6 : 2 : 2 entities Detail

Interaction partners 1. mSin3B PAH1, : 9 interactors Detail

More details for 9 interactors identified by 8 citations

Interaction partners 2. NRSF/REST, : 13 interactors Detail

More details for 13 interactors identified by 7 citations

Experiments performed 14 experiments Detail

1 1H-15N HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Temperature 293 (±0.2) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	mSin3B PAH1	[U-13C; U-15N]		0.8 mM
2	NRSF/REST			0.8 mM
3	potassium phosphate buffer			20 mM

2 HNCO

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Temperature 293 (±0.2) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	mSin3B PAH1	[U-13C; U-15N]		0.8 mM
2	NRSF/REST			0.8 mM
3	potassium phosphate buffer			20 mM

3 HN(CA)CO

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Temperature 293 (±0.2) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	mSin3B PAH1	[U-13C; U-15N]		0.8 mM
2	NRSF/REST			0.8 mM
3	potassium phosphate buffer			20 mM

4 HNCA

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Temperature 293 (±0.2) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	mSin3B PAH1	[U-13C; U-15N]		0.8 mM
2	NRSF/REST			0.8 mM
3	potassium phosphate buffer			20 mM

5 HN(CO)CA

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Temperature 293 (±0.2) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	mSin3B PAH1	[U-13C; U-15N]		0.8 mM
2	NRSF/REST			0.8 mM
3	potassium phosphate buffer			20 mM

6 CBCA(CO)NH

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Temperature 293 (±0.2) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	mSin3B PAH1	[U-13C; U-15N]		0.8 mM
2	NRSF/REST			0.8 mM
3	potassium phosphate buffer			20 mM

7 HNCACB

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Temperature 293 (±0.2) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	mSin3B PAH1	[U-13C; U-15N]		0.8 mM
2	NRSF/REST			0.8 mM
3	potassium phosphate buffer			20 mM

8 HBHA(CO)NH

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Temperature 293 (±0.2) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	mSin3B PAH1	[U-13C; U-15N]		0.8 mM
2	NRSF/REST			0.8 mM
3	potassium phosphate buffer			20 mM

9 HN(CA)HA

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Temperature 293 (±0.2) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	mSin3B PAH1	[U-13C; U-15N]		0.8 mM
2	NRSF/REST			0.8 mM
3	potassium phosphate buffer			20 mM

10 1H-13C HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Temperature 293 (±0.2) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	mSin3B PAH1	[U-13C; U-15N]		0.8 mM
2	NRSF/REST			0.8 mM
3	potassium phosphate buffer			20 mM

11 HCCH-TOCSY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Temperature 293 (±0.2) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	mSin3B PAH1	[U-13C; U-15N]		0.8 mM
2	NRSF/REST			0.8 mM
3	potassium phosphate buffer			20 mM

12 CCH-TOCSY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Temperature 293 (±0.2) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	mSin3B PAH1	[U-13C; U-15N]		0.8 mM
2	NRSF/REST			0.8 mM
3	potassium phosphate buffer			20 mM

13 13C/15N-FILTERED-NOESY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Temperature 293 (±0.2) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	mSin3B PAH1	[U-13C; U-15N]		0.8 mM
2	NRSF/REST			0.8 mM
3	potassium phosphate buffer			20 mM

14 13C/15N-FILTERED-TOCSY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Temperature 293 (±0.2) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	mSin3B PAH1	[U-13C; U-15N]		0.8 mM
2	NRSF/REST			0.8 mM
3	potassium phosphate buffer			20 mM

nullKeywords NRSF, PAH1, Sin3, transcriptional repressor