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Found 1 Document (2.229 seconds)

BMRB: 6751

2AFD

Solution Structure of Asl1650, an Acyl Carrier Protein from Anabaena sp. PCC 7120 with a Variant Phosphopantetheinylation-Site Sequence

Authors

Johnson, M.A., Peti, W., Herrmann, T., Wilson, I.A., Wuthrich, K.

Assembly

Asl1650

Entity

1. Asl1650 (polymer, Thiol state: not present), 88 monomers, 10080.33 Da Detail

GSHMKTIQPA SVEDIQSWLI DQFAQQLDVD PDDIDMEESF DNYDLNSSKA LILLGRLEKW LGKELNPVLI FNYPTIAQLA KRLGELYL

Formula weight

10080.33 Da

Source organism

Nostoc sp. PCC 7120 = FACHB-418

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.6 %, Completeness (bb): 97.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.6 % (1004 of 1061)	98.7 % (545 of 552)	88.4 % (365 of 413)	97.9 % (94 of 96)
Backbone	97.9 % (509 of 520)	98.9 % (174 of 176)	97.3 % (253 of 260)	97.6 % (82 of 84)
Sidechain	92.6 % (579 of 625)	98.7 % (371 of 376)	82.7 % (196 of 237)	100.0 % (12 of 12)
Aromatic	51.2 % (42 of 82)	97.6 % (40 of 41)	0.0 % (0 of 39)	100.0 % (2 of 2)
Methyl	100.0 % (110 of 110)	100.0 % (55 of 55)	100.0 % (55 of 55)

1. Asl1650

GSHMKTIQPA SVEDIQSWLI DQFAQQLDVD PDDIDMEESF DNYDLNSSKA LILLGRLEKW LGKELNPVLI FNYPTIAQLA KRLGELYL

Show sample condition

Sample

Temperature 303 (±0.02) K, pH 6.0 (±0.2), Details 2 mM NaN3 added to prevent microbial growth

#	Name	Isotope labeling	Concentration
1	Asl1650	[U-15N; U-13C]	2 mM
2	phosphate buffer		20 mM
3	NaCl		250 mM
4	NaN3		2 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.39 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 1 models in PDB: 2AFE, Strand ID: A Detail

Release date

2005-08-21

Citation

Solution structure of Asl1650, an acyl carrier protein from Anabaena sp. PCC 7120 with a variant phosphopantetheinylation-site sequence
Johnson, M.A., Peti, W., Herrmann, T., Wilson, I.A., Wuthrich, K.
Protein Sci. (2006), 15, 1030-1041, PubMed 16597827 , DOI 10.1110/ps.051964606 , Abstract

Related entities 1. Asl1650, : 1 : 1 : 41 entities Detail

Experiments performed 12 experiments Detail

1 3D HNCACB

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (900 MHz)

Sample #1

Temperature 303 (±0.02) K, pH 6.0 (±0.2), Details 2 mM NaN3 added to prevent microbial growth

#	Name	Isotope labeling	Concentration
1	Asl1650	[U-15N; U-13C]	2 mM
2	phosphate buffer		20 mM
3	NaCl		250 mM
4	NaN3		2 mM

Sample #2

Temperature 303 (±0.02) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	Asl1650	[U-15N]	2 mM
6	phosphate buffer		20 mM
7	NaCl		250 mM
8	NaN3		2 mM

Sample #3

Temperature 303 (±0.02) K, pH 6.0 (±0.2)

#	Name	Concentration
9	Asl1650	2 mM
10	phosphate buffer	20 mM
11	NaCl	250 mM
12	NaN3	2 mM

2 CBCA(CO)NH

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (900 MHz)

Sample #1

Temperature 303 (±0.02) K, pH 6.0 (±0.2), Details 2 mM NaN3 added to prevent microbial growth

#	Name	Isotope labeling	Concentration
1	Asl1650	[U-15N; U-13C]	2 mM
2	phosphate buffer		20 mM
3	NaCl		250 mM
4	NaN3		2 mM

Sample #2

Temperature 303 (±0.02) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	Asl1650	[U-15N]	2 mM
6	phosphate buffer		20 mM
7	NaCl		250 mM
8	NaN3		2 mM

Sample #3

Temperature 303 (±0.02) K, pH 6.0 (±0.2)

#	Name	Concentration
9	Asl1650	2 mM
10	phosphate buffer	20 mM
11	NaCl	250 mM
12	NaN3	2 mM

3 HNCO

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (900 MHz)

Sample #1

Temperature 303 (±0.02) K, pH 6.0 (±0.2), Details 2 mM NaN3 added to prevent microbial growth

#	Name	Isotope labeling	Concentration
1	Asl1650	[U-15N; U-13C]	2 mM
2	phosphate buffer		20 mM
3	NaCl		250 mM
4	NaN3		2 mM

Sample #2

Temperature 303 (±0.02) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	Asl1650	[U-15N]	2 mM
6	phosphate buffer		20 mM
7	NaCl		250 mM
8	NaN3		2 mM

Sample #3

Temperature 303 (±0.02) K, pH 6.0 (±0.2)

#	Name	Concentration
9	Asl1650	2 mM
10	phosphate buffer	20 mM
11	NaCl	250 mM
12	NaN3	2 mM

4 HBHA(CO)NH

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (900 MHz)

Sample #1

Temperature 303 (±0.02) K, pH 6.0 (±0.2), Details 2 mM NaN3 added to prevent microbial growth

#	Name	Isotope labeling	Concentration
1	Asl1650	[U-15N; U-13C]	2 mM
2	phosphate buffer		20 mM
3	NaCl		250 mM
4	NaN3		2 mM

Sample #2

Temperature 303 (±0.02) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	Asl1650	[U-15N]	2 mM
6	phosphate buffer		20 mM
7	NaCl		250 mM
8	NaN3		2 mM

Sample #3

Temperature 303 (±0.02) K, pH 6.0 (±0.2)

#	Name	Concentration
9	Asl1650	2 mM
10	phosphate buffer	20 mM
11	NaCl	250 mM
12	NaN3	2 mM

5 15N-resolved [1H,1H]-TOCSY

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (900 MHz)

Sample #1

Temperature 303 (±0.02) K, pH 6.0 (±0.2), Details 2 mM NaN3 added to prevent microbial growth

#	Name	Isotope labeling	Concentration
1	Asl1650	[U-15N; U-13C]	2 mM
2	phosphate buffer		20 mM
3	NaCl		250 mM
4	NaN3		2 mM

Sample #2

Temperature 303 (±0.02) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	Asl1650	[U-15N]	2 mM
6	phosphate buffer		20 mM
7	NaCl		250 mM
8	NaN3		2 mM

Sample #3

Temperature 303 (±0.02) K, pH 6.0 (±0.2)

#	Name	Concentration
9	Asl1650	2 mM
10	phosphate buffer	20 mM
11	NaCl	250 mM
12	NaN3	2 mM

6 HC(CCO)NH

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (900 MHz)

Sample #1

Temperature 303 (±0.02) K, pH 6.0 (±0.2), Details 2 mM NaN3 added to prevent microbial growth

#	Name	Isotope labeling	Concentration
1	Asl1650	[U-15N; U-13C]	2 mM
2	phosphate buffer		20 mM
3	NaCl		250 mM
4	NaN3		2 mM

Sample #2

Temperature 303 (±0.02) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	Asl1650	[U-15N]	2 mM
6	phosphate buffer		20 mM
7	NaCl		250 mM
8	NaN3		2 mM

Sample #3

Temperature 303 (±0.02) K, pH 6.0 (±0.2)

#	Name	Concentration
9	Asl1650	2 mM
10	phosphate buffer	20 mM
11	NaCl	250 mM
12	NaN3	2 mM

7 HC(C)H-TOCSY

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (900 MHz)

Sample #1

Temperature 303 (±0.02) K, pH 6.0 (±0.2), Details 2 mM NaN3 added to prevent microbial growth

#	Name	Isotope labeling	Concentration
1	Asl1650	[U-15N; U-13C]	2 mM
2	phosphate buffer		20 mM
3	NaCl		250 mM
4	NaN3		2 mM

Sample #2

Temperature 303 (±0.02) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	Asl1650	[U-15N]	2 mM
6	phosphate buffer		20 mM
7	NaCl		250 mM
8	NaN3		2 mM

Sample #3

Temperature 303 (±0.02) K, pH 6.0 (±0.2)

#	Name	Concentration
9	Asl1650	2 mM
10	phosphate buffer	20 mM
11	NaCl	250 mM
12	NaN3	2 mM

8 2D [1H,1H]-COSY

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (900 MHz)

Sample #1

Temperature 303 (±0.02) K, pH 6.0 (±0.2), Details 2 mM NaN3 added to prevent microbial growth

#	Name	Isotope labeling	Concentration
1	Asl1650	[U-15N; U-13C]	2 mM
2	phosphate buffer		20 mM
3	NaCl		250 mM
4	NaN3		2 mM

Sample #2

Temperature 303 (±0.02) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	Asl1650	[U-15N]	2 mM
6	phosphate buffer		20 mM
7	NaCl		250 mM
8	NaN3		2 mM

Sample #3

Temperature 303 (±0.02) K, pH 6.0 (±0.2)

#	Name	Concentration
9	Asl1650	2 mM
10	phosphate buffer	20 mM
11	NaCl	250 mM
12	NaN3	2 mM

9 2D [1H,1H]-TOCSY

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (900 MHz)

Sample #1

Temperature 303 (±0.02) K, pH 6.0 (±0.2), Details 2 mM NaN3 added to prevent microbial growth

#	Name	Isotope labeling	Concentration
1	Asl1650	[U-15N; U-13C]	2 mM
2	phosphate buffer		20 mM
3	NaCl		250 mM
4	NaN3		2 mM

Sample #2

Temperature 303 (±0.02) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	Asl1650	[U-15N]	2 mM
6	phosphate buffer		20 mM
7	NaCl		250 mM
8	NaN3		2 mM

Sample #3

Temperature 303 (±0.02) K, pH 6.0 (±0.2)

#	Name	Concentration
9	Asl1650	2 mM
10	phosphate buffer	20 mM
11	NaCl	250 mM
12	NaN3	2 mM

10 2D [1H,1H]-NOESY

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (900 MHz)

Sample #1

Temperature 303 (±0.02) K, pH 6.0 (±0.2), Details 2 mM NaN3 added to prevent microbial growth

#	Name	Isotope labeling	Concentration
1	Asl1650	[U-15N; U-13C]	2 mM
2	phosphate buffer		20 mM
3	NaCl		250 mM
4	NaN3		2 mM

Sample #2

Temperature 303 (±0.02) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	Asl1650	[U-15N]	2 mM
6	phosphate buffer		20 mM
7	NaCl		250 mM
8	NaN3		2 mM

Sample #3

Temperature 303 (±0.02) K, pH 6.0 (±0.2)

#	Name	Concentration
9	Asl1650	2 mM
10	phosphate buffer	20 mM
11	NaCl	250 mM
12	NaN3	2 mM

11 3D 15N-resolved [1H,1H]-NOESY

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (900 MHz)

Sample #1

Temperature 303 (±0.02) K, pH 6.0 (±0.2), Details 2 mM NaN3 added to prevent microbial growth

#	Name	Isotope labeling	Concentration
1	Asl1650	[U-15N; U-13C]	2 mM
2	phosphate buffer		20 mM
3	NaCl		250 mM
4	NaN3		2 mM

Sample #2

Temperature 303 (±0.02) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	Asl1650	[U-15N]	2 mM
6	phosphate buffer		20 mM
7	NaCl		250 mM
8	NaN3		2 mM

Sample #3

Temperature 303 (±0.02) K, pH 6.0 (±0.2)

#	Name	Concentration
9	Asl1650	2 mM
10	phosphate buffer	20 mM
11	NaCl	250 mM
12	NaN3	2 mM

12 3D 13C-resolved [1H,1H]-NOESY

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (900 MHz)

Sample #1

Temperature 303 (±0.02) K, pH 6.0 (±0.2), Details 2 mM NaN3 added to prevent microbial growth

#	Name	Isotope labeling	Concentration
1	Asl1650	[U-15N; U-13C]	2 mM
2	phosphate buffer		20 mM
3	NaCl		250 mM
4	NaN3		2 mM

Sample #2

Temperature 303 (±0.02) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	Asl1650	[U-15N]	2 mM
6	phosphate buffer		20 mM
7	NaCl		250 mM
8	NaN3		2 mM

Sample #3

Temperature 303 (±0.02) K, pH 6.0 (±0.2)

#	Name	Concentration
9	Asl1650	2 mM
10	phosphate buffer	20 mM
11	NaCl	250 mM
12	NaN3	2 mM

nullKeywords acyl carrier protein family, hypothetical protein

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Found 1 Document (2.229 seconds)

BMRB: 6751

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Asl1650, : 1 : 1 : 41 entities Detail

Experiments performed 12 experiments Detail

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