BMRBj - BMREntryMaster

Found 1 Document (4.013 seconds)

BMRB: 6752

1Z87

Backbone and sidechain 1H, 13C, and 15N Chemical Shift Assignments for the PH-PDZ tandem of alpha syntrophin

Authors

Yan, J., Wen, W., Xu, W., Long, J., Adams, M.E., Froehner, S.C., Zhang, M.

Assembly

PH-PDZ tandem of alpha-syntrophin

Entity

1. PH-PDZ tandem of alpha-syntrophin (polymer, Thiol state: all free), 263 monomers, 27681.98 Da Detail

ASGRRAPRTG LLELRCGAGS GAGGERWQRV LLSLAEDALT VSPADGEPGP EPEPAQLNGA AEPGAAPPQL PEALLLQRRR VTVRKADAGG LGISIKGGRE NKMPILISKI FKGLAADQTE ALFVGDAILS VNGEDLSSAT HDEAVQALKK TGKEVVLEVK YMKEVSPYFK NSAGGTSVGW DSPPASPLQR QPSSPGPQPR NLSEAKHVSL KMAYVSRRCT PTDPEPRYLE ICAADGQDAV FLRAKDEASA RSWAGAIQAQ IGT

Formula weight

27681.98 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 83.0 %, Completeness (bb): 83.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.0 % (2398 of 2889)	92.8 % (1402 of 1510)	66.4 % (742 of 1118)	97.3 % (254 of 261)
Backbone	83.1 % (1275 of 1534)	97.4 % (517 of 531)	68.6 % (523 of 762)	97.5 % (235 of 241)
Sidechain	83.6 % (1330 of 1591)	90.4 % (885 of 979)	72.0 % (426 of 592)	95.0 % (19 of 20)
Aromatic	38.8 % (45 of 116)	72.4 % (42 of 58)	0.0 % (0 of 55)	100.0 % (3 of 3)
Methyl	96.5 % (276 of 286)	99.3 % (142 of 143)	93.7 % (134 of 143)

1. PH-PDZ tandem

Show sample condition

Sample

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PH-PDZ tandem	[U-13C; U-15N]		1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.74 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.74 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 1Z87, Strand ID: A Detail

Release date

2007-01-28

Citation

Structure of the split PH domain and distinct lipid-binding properties of the PH-PDZ supramodule of alpha-syntrophin
Yan, J., Wen, W., Xu, W., Long, J., Adams, M.E., Froehner, S.C., Zhang, M.
EMBO J. (2005), 24, 3985-3995, PubMed 16252003 , DOI 10.1038/sj.emboj.7600858 , Abstract

Related entities 1. PH-PDZ tandem of alpha-syntrophin, : 1 : 2 : 19 entities Detail

Interaction partners 1. PH-PDZ tandem of alpha-syntrophin, : 15 interactors Detail

More details for 15 interactors identified by 9 citations

Experiments performed 8 experiments Detail

1 HNCO

Instrument

Model Varian Inova (750 MHz)

Sample #1

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PH-PDZ tandem	[U-13C; U-15N]		1 mM

Sample #2

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
2	PH-PDZ tandem	[U-15N]		1 mM

2 HNCA

Instrument

Model Varian Inova (750 MHz)

Sample #1

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PH-PDZ tandem	[U-13C; U-15N]		1 mM

Sample #2

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
2	PH-PDZ tandem	[U-15N]		1 mM

3 HN(CO)CA

Instrument

Model Varian Inova (750 MHz)

Sample #1

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PH-PDZ tandem	[U-13C; U-15N]		1 mM

Sample #2

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
2	PH-PDZ tandem	[U-15N]		1 mM

4 HNCACB

Instrument

Model Varian Inova (750 MHz)

Sample #1

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PH-PDZ tandem	[U-13C; U-15N]		1 mM

Sample #2

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
2	PH-PDZ tandem	[U-15N]		1 mM

5 CBCA(CO)NH

Instrument

Model Varian Inova (750 MHz)

Sample #1

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PH-PDZ tandem	[U-13C; U-15N]		1 mM

Sample #2

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
2	PH-PDZ tandem	[U-15N]		1 mM

6 HCCH-TOCSY

Instrument

Model Varian Inova (750 MHz)

Sample #1

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PH-PDZ tandem	[U-13C; U-15N]		1 mM

Sample #2

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
2	PH-PDZ tandem	[U-15N]		1 mM

7 3D 15N-separated NOESY

Instrument

Model Varian Inova (750 MHz)

Sample #1

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PH-PDZ tandem	[U-13C; U-15N]		1 mM

Sample #2

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
2	PH-PDZ tandem	[U-15N]		1 mM

8 3D 15C-separated NOESY

Instrument

Model Varian Inova (750 MHz)

Sample #1

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
1	PH-PDZ tandem	[U-13C; U-15N]		1 mM

Sample #2

Temperature 303 K, pH 7 (±0.5)

#	Name	Isotope labeling	Type	Concentration
2	PH-PDZ tandem	[U-15N]		1 mM

nullKeywords PH-PDZ tandem, split PH domain, syntrophin, NMR structure

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Found 1 Document (4.013 seconds)

BMRB: 6752

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. PH-PDZ tandem of alpha-syntrophin, : 1 : 2 : 19 entities Detail

Interaction partners 1. PH-PDZ tandem of alpha-syntrophin, : 15 interactors Detail

Experiments performed 8 experiments Detail

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