BMRB: 6755

Backbone 1H, 13C, and 15N Chemical Shift Assignments for TA0743

Authors

Kim, B.

Assembly

TA0743

Entity

1. TA0743 (polymer, Thiol state: not present), 146 monomers, 17368.36 Da Detail

MGTSHHHHHH SSGRENLYFQ SHMQKGLEIA FQTINGLDES LVQALAGVTA SDFPDLDIKY NIFLVDLYGQ KYFRILFQSK KLSELHPEER KKVREKFDEN SRMQYSELMT KYHDLKKQGK IKDRPVKEVH EEYDLWEDPI WQYIGS

Formula weight

17368.36 Da

Source organism

Thermoplasma acidophilum

Exptl. method

NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 84.9 %, Completeness: 68.3 %, Completeness (bb): 69.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	68.3 % (1235 of 1809)	77.0 % (732 of 951)	55.2 % (387 of 701)	73.9 % (116 of 157)
Backbone	69.5 % (603 of 868)	81.4 % (241 of 296)	57.9 % (249 of 430)	79.6 % (113 of 142)
Sidechain	69.3 % (748 of 1079)	75.1 % (492 of 655)	61.9 % (253 of 409)	20.0 % (3 of 15)
Aromatic	29.8 % (59 of 198)	56.6 % (56 of 99)	1.0 % (1 of 97)	100.0 % (2 of 2)
Methyl	85.9 % (110 of 128)	89.1 % (57 of 64)	82.8 % (53 of 64)

1. TA0743

MGTSHHHHHH SSGRENLYFQ SHMQKGLEIA FQTINGLDES LVQALAGVTA SDFPDLDIKY NIFLVDLYGQ KYFRILFQSK KLSELHPEER KKVREKFDEN SRMQYSELMT KYHDLKKQGK IKDRPVKEVH EEYDLWEDPI WQYIGS

Show sample condition

Sample

Pressure 1 (±0) atm, Temperature 298 (±0) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	TA0743	[U-99% 13C; U-99% 15N]	protein	1.6 mM

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LACS Plot; CA

Referencing offset: -0.06 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.06 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.04 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2ABY, Strand ID: A Detail

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Release date

2006-04-23

Citation

NMR structure of the conserved novel-fold protein TA0743 from Thermoplasma acidophilum
Kim, B., Jung, J., Hong, E., Yee, A., Arrowsmith, C.H., Lee, W.
Proteins (2006), 62, 819-821, PubMed 16374782 , DOI 10.1002/prot.20832 , Abstract

Related entities 1. TA0743, : 1 entities Detail

Experiments performed 7 experiments Detail