BMRBj - BMREntryMaster

	Total	¹H	¹³C
All	89.3 % (283 of 317)	97.2 % (176 of 181)	78.7 % (107 of 136)
Backbone	78.6 % (110 of 140)	98.3 % (58 of 59)	64.2 % (52 of 81)
Sidechain	97.5 % (197 of 202)	96.7 % (118 of 122)	98.8 % (79 of 80)
Aromatic	100.0 % (31 of 31)	100.0 % (16 of 16)	100.0 % (15 of 15)
Methyl	100.0 % (42 of 42)	100.0 % (21 of 21)	100.0 % (21 of 21)

1. BVDV NS5A[1-28]

SGNYVLDLIY SLHKQINRGL KKIVLGWA

Show sample condition

Sample

Temperature 303 (±1) K, pH 4.5 (±0.2), Details Sample in TriFluoroEthanol (TFE) 50%

#	Name	Isotope labeling	Type	Concentration
1	BVDV NS5A[1-28]		protein	2 (±0.1) mM
2	2,2,2-TriFluoroEthanol-d2	TFE-d2 (>99%)	solvent	50 (±1.0) % v/v
3	water		solvent	50 (±1.0) % v/v
4	2,2-dimethyl-2-silapentane-5-sulfonic acid		internal reference	0.01 mM

Chem. Shift Complete2

Sequence coverage: 100.0 %, Completeness: 88.5 %, Completeness (bb): 77.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C
All	88.5 % (561 of 634)	98.1 % (355 of 362)	75.7 % (206 of 272)
Backbone	77.1 % (216 of 280)	98.3 % (116 of 118)	61.7 % (100 of 162)
Sidechain	97.3 % (393 of 404)	98.0 % (239 of 244)	96.2 % (154 of 160)
Aromatic	95.2 % (59 of 62)	100.0 % (32 of 32)	90.0 % (27 of 30)
Methyl	100.0 % (84 of 84)	100.0 % (42 of 42)	100.0 % (42 of 42)

1. BVDV NS5A[1-28]

SGNYVLDLIY SLHKQINRGL KKIVLGWA

Show sample condition

Sample

Temperature 303 (±1) K, pH 4.5 (±0.2), Details Sample in micellar Sodium Dodecyl Sulfate (SDS) 100 mM

#	Name	Isotope labeling	Type	Concentration
5	BVDV NS5A[1-28]		protein	2 (±0.1) mM
6	Sodium Dodecyl Sulfate (SDS)-d25	SDS-d25 (>98%)	lipid	100 (±1.0) mM
7	water		solvent	100 (±1.0) % v/v
8	2,2-dimethyl-2-silapentane-5-sulfonic acid		internal reference	0.01 mM

Protein Blocks Logo

Calculated from 31 models in PDB: 2AJJ, Strand ID: A Detail

Release date

2006-04-23

Citation

NMR structure and molecular dynamics of the in-plane membrane anchor of nonstructural protein 5A from bovine viral diarrhea virus
Sapay, N., Montserret, R., Chipot, C., Brass, V., Moradpour, D., Deleage, G., Penin, F.
Biochemistry (2006), 45, 2221-2233, PubMed 16475810 , DOI 10.1021/bi0517685 , Abstract

Related entities 1. membrane anchor domain of BVDV NS5A, : 1 : 4 : 10 entities Detail

Interaction partners 1. membrane anchor domain of BVDV NS5A, : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 6 experiments Detail

1 TOCSY in TFE 50%

Instrument

Model Varian Unity-plus (500 MHz)

Sample #1

pH 4.5 (±0.2), Details Sample in TriFluoroEthanol (TFE) 50%

#	Name	Isotope labeling	Type	Concentration
1	BVDV NS5A[1-28]		protein	2 (±0.1) mM
2	2,2,2-TriFluoroEthanol-d2	TFE-d2 (>99%)	solvent	50 (±1.0) % v/v
3	water		solvent	50 (±1.0) % v/v
4	2,2-dimethyl-2-silapentane-5-sulfonic acid		internal reference	0.01 mM

Sample #2

pH 4.5 (±0.2), Details Sample in micellar Sodium Dodecyl Sulfate (SDS) 100 mM

#	Name	Isotope labeling	Type	Concentration
5	BVDV NS5A[1-28]		protein	2 (±0.1) mM
6	Sodium Dodecyl Sulfate (SDS)-d25	SDS-d25 (>98%)	lipid	100 (±1.0) mM
7	water		solvent	100 (±1.0) % v/v
8	2,2-dimethyl-2-silapentane-5-sulfonic acid		internal reference	0.01 mM

2 1H_13C_HSQC in TFE 50%

Instrument

Model Varian Unity-plus (500 MHz)

Sample #1

pH 4.5 (±0.2), Details Sample in TriFluoroEthanol (TFE) 50%

#	Name	Isotope labeling	Type	Concentration
1	BVDV NS5A[1-28]		protein	2 (±0.1) mM
2	2,2,2-TriFluoroEthanol-d2	TFE-d2 (>99%)	solvent	50 (±1.0) % v/v
3	water		solvent	50 (±1.0) % v/v
4	2,2-dimethyl-2-silapentane-5-sulfonic acid		internal reference	0.01 mM

Sample #2

pH 4.5 (±0.2), Details Sample in micellar Sodium Dodecyl Sulfate (SDS) 100 mM

#	Name	Isotope labeling	Type	Concentration
5	BVDV NS5A[1-28]		protein	2 (±0.1) mM
6	Sodium Dodecyl Sulfate (SDS)-d25	SDS-d25 (>98%)	lipid	100 (±1.0) mM
7	water		solvent	100 (±1.0) % v/v
8	2,2-dimethyl-2-silapentane-5-sulfonic acid		internal reference	0.01 mM

3 NOESY in TFE 50%

Instrument

Model Varian Unity-plus (500 MHz)

Sample #1

pH 4.5 (±0.2), Details Sample in TriFluoroEthanol (TFE) 50%

#	Name	Isotope labeling	Type	Concentration
1	BVDV NS5A[1-28]		protein	2 (±0.1) mM
2	2,2,2-TriFluoroEthanol-d2	TFE-d2 (>99%)	solvent	50 (±1.0) % v/v
3	water		solvent	50 (±1.0) % v/v
4	2,2-dimethyl-2-silapentane-5-sulfonic acid		internal reference	0.01 mM

Sample #2

pH 4.5 (±0.2), Details Sample in micellar Sodium Dodecyl Sulfate (SDS) 100 mM

#	Name	Isotope labeling	Type	Concentration
5	BVDV NS5A[1-28]		protein	2 (±0.1) mM
6	Sodium Dodecyl Sulfate (SDS)-d25	SDS-d25 (>98%)	lipid	100 (±1.0) mM
7	water		solvent	100 (±1.0) % v/v
8	2,2-dimethyl-2-silapentane-5-sulfonic acid		internal reference	0.01 mM

4 TOCSY in SDS 100 mM

Instrument

Model Varian Unity-plus (500 MHz)

Sample #1

pH 4.5 (±0.2), Details Sample in TriFluoroEthanol (TFE) 50%

#	Name	Isotope labeling	Type	Concentration
1	BVDV NS5A[1-28]		protein	2 (±0.1) mM
2	2,2,2-TriFluoroEthanol-d2	TFE-d2 (>99%)	solvent	50 (±1.0) % v/v
3	water		solvent	50 (±1.0) % v/v
4	2,2-dimethyl-2-silapentane-5-sulfonic acid		internal reference	0.01 mM

Sample #2

pH 4.5 (±0.2), Details Sample in micellar Sodium Dodecyl Sulfate (SDS) 100 mM

#	Name	Isotope labeling	Type	Concentration
5	BVDV NS5A[1-28]		protein	2 (±0.1) mM
6	Sodium Dodecyl Sulfate (SDS)-d25	SDS-d25 (>98%)	lipid	100 (±1.0) mM
7	water		solvent	100 (±1.0) % v/v
8	2,2-dimethyl-2-silapentane-5-sulfonic acid		internal reference	0.01 mM

5 1H_13C_HSQC in SDS 100 mM

Instrument

Model Varian Unity-plus (500 MHz)

Sample #1

pH 4.5 (±0.2), Details Sample in TriFluoroEthanol (TFE) 50%

#	Name	Isotope labeling	Type	Concentration
1	BVDV NS5A[1-28]		protein	2 (±0.1) mM
2	2,2,2-TriFluoroEthanol-d2	TFE-d2 (>99%)	solvent	50 (±1.0) % v/v
3	water		solvent	50 (±1.0) % v/v
4	2,2-dimethyl-2-silapentane-5-sulfonic acid		internal reference	0.01 mM

Sample #2

pH 4.5 (±0.2), Details Sample in micellar Sodium Dodecyl Sulfate (SDS) 100 mM

#	Name	Isotope labeling	Type	Concentration
5	BVDV NS5A[1-28]		protein	2 (±0.1) mM
6	Sodium Dodecyl Sulfate (SDS)-d25	SDS-d25 (>98%)	lipid	100 (±1.0) mM
7	water		solvent	100 (±1.0) % v/v
8	2,2-dimethyl-2-silapentane-5-sulfonic acid		internal reference	0.01 mM

6 NOESY in SDS 100 mM

Instrument

Model Varian Unity-plus (500 MHz)

Sample #1

pH 4.5 (±0.2), Details Sample in TriFluoroEthanol (TFE) 50%

#	Name	Isotope labeling	Type	Concentration
1	BVDV NS5A[1-28]		protein	2 (±0.1) mM
2	2,2,2-TriFluoroEthanol-d2	TFE-d2 (>99%)	solvent	50 (±1.0) % v/v
3	water		solvent	50 (±1.0) % v/v
4	2,2-dimethyl-2-silapentane-5-sulfonic acid		internal reference	0.01 mM

Sample #2

pH 4.5 (±0.2), Details Sample in micellar Sodium Dodecyl Sulfate (SDS) 100 mM

#	Name	Isotope labeling	Type	Concentration
5	BVDV NS5A[1-28]		protein	2 (±0.1) mM
6	Sodium Dodecyl Sulfate (SDS)-d25	SDS-d25 (>98%)	lipid	100 (±1.0) mM
7	water		solvent	100 (±1.0) % v/v
8	2,2-dimethyl-2-silapentane-5-sulfonic acid		internal reference	0.01 mM

nullKeywords amphipathic helix, in-plane membrane anchor domain, amphipathic alpha-helix, liquid NMR, micellar SDS, water:TFE mixture

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BMRB: 6757

Sample

Sample

Related entities 1. membrane anchor domain of BVDV NS5A, : 1 : 4 : 10 entities Detail

Interaction partners 1. membrane anchor domain of BVDV NS5A, : 2 interactors Detail

Experiments performed 6 experiments Detail

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