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BMRB: 6759

2H3K

NEAT NMR Resonance Assignments

Authors

Pilpa, R.M., Clubb, R.T.

Assembly

NEAT domain

Entity

1. NEAT domain (polymer, Thiol state: not present), 145 monomers, 16844.47 Da Detail

MADESLKDAI KDPALENKEH DIGPREQVNF QLLDKNNETQ YYHFFSIKDP ADVYYTKKKA EVELDINTAS TWKKFEVYEN NQKLPVRLVS YSPVPEDHAY IRFPVSDGTQ ELKIVSSTQI DDGEETNYDY TKLVFAKPIY NDPSL

Formula weight

16844.47 Da

Source organism

Staphylococcus aureus

Exptl. method

NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.6 %, Completeness: 79.2 %, Completeness (bb): 90.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	79.2 % (1383 of 1746)	75.8 % (691 of 912)	83.1 % (567 of 682)	82.2 % (125 of 152)
Backbone	90.8 % (774 of 852)	91.5 % (260 of 284)	90.5 % (391 of 432)	90.4 % (123 of 136)
Sidechain	71.9 % (745 of 1036)	68.6 % (431 of 628)	79.6 % (312 of 392)	12.5 % (2 of 16)
Aromatic	35.4 % (58 of 164)	36.6 % (30 of 82)	33.3 % (27 of 81)	100.0 % (1 of 1)
Methyl	97.9 % (141 of 144)	97.2 % (70 of 72)	98.6 % (71 of 72)

1. NEAT domain

MADESLKDAI KDPALENKEH DIGPREQVNF QLLDKNNETQ YYHFFSIKDP ADVYYTKKKA EVELDINTAS TWKKFEVYEN NQKLPVRLVS YSPVPEDHAY IRFPVSDGTQ ELKIVSSTQI DDGEETNYDY TKLVFAKPIY NDPSL

Show sample condition

Sample

Temperature 295 (±1) K, pH 6 (±0.2), Details The NEAT protein was at 1mM concentration in 50mM sodium phospate buffer pH 6, 100mM NaCl, and 0.01% sodium azide

#	Name	Isotope labeling	Type	Concentration
1	NEAT domain	[U-13C; U-15N]	protein	1 mM
2	sodium phospate		buffer	50 mM
3	NaCl		salt	100 mM
4	sodium azide		antimicrobial agent	0.01 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.26 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.26 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 5 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.78 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2H3K, Strand ID: A Detail

Release date

2005-08-02

Citation

NMR resonance assignments of the NEAT (NEAr Transporter) domain from the Staphylococcus aureus IsdH protein
Pilpa, R.M., Clubb, R.T.
J. Biomol. NMR (2005), 33, 137-137, PubMed 16258836 , DOI 10.1007/s10858-005-2898-2 , Abstract

Related entities 1. NEAT domain, : 1 : 3 : 7 : 25 entities Detail

Experiments performed 18 experiments Detail

1 1H15N_HSQC