BMRB: 6760

NMR assignment of the mTOR domain responsible for rapamycin binding

Authors

Veverka, V., Lennie, G., Crabbe, T., Bird, I., Taylor, R.J., Carr, M.D.

Assembly

FRB domain

Entity

1. FRB domain (polymer, Thiol state: all free), 126 monomers, 15337.21 Da Detail

MSYYHHHHHH DYDIPTTENL YFQGAMELIR VPILWHEMWH EGLEEASRLY FGERNVKGMF EVLEPLHAMM ERGPQTLKET SFNQAYGRDL MEAQEWCRKY MKSGNVKDLT QAWDLYYHVF RRISKQ

Formula weight

15337.21 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.8 %, Completeness: 75.1 %, Completeness (bb): 80.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	75.1 % (1172 of 1561)	84.4 % (689 of 816)	60.1 % (366 of 609)	86.0 % (117 of 136)
Backbone	80.6 % (603 of 748)	94.9 % (242 of 255)	66.8 % (248 of 371)	92.6 % (113 of 122)
Sidechain	72.9 % (679 of 932)	79.7 % (447 of 561)	63.9 % (228 of 357)	28.6 % (4 of 14)
Aromatic	37.1 % (78 of 210)	68.6 % (72 of 105)	2.0 % (2 of 101)	100.0 % (4 of 4)
Methyl	100.0 % (102 of 102)	100.0 % (51 of 51)	100.0 % (51 of 51)

1. FRB domain

MSYYHHHHHH DYDIPTTENL YFQGAMELIR VPILWHEMWH EGLEEASRLY FGERNVKGMF EVLEPLHAMM ERGPQTLKET SFNQAYGRDL MEAQEWCRKY MKSGNVKDLT QAWDLYYHVF RRISKQ

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	FRB domain	[U-15N]		0.1 mM

Sample #2

Temperature 298 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	FRB domain	[U-15N; U-13C]		0.1 mM

Sample #3

Temperature 298 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	FRB domain			0.1 mM

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LACS Plot; CA

Referencing offset: -0.06 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.06 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.12 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 32 models in PDB: 2NPU, Strand ID: A Detail

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Release date

2006-03-09

Citation

NMR assignment of the mTOR domain responsible for rapamycin binding
Veverka, V., Lennie, G., Crabbe, T., Bird, I., Taylor, R.J., Carr, M.D.
J. Biomol. NMR (2006), 36 Suppl 1, 3-3, PubMed 16456707 , DOI 10.1007/s10858-005-4324-1 , Abstract

Related entities 1. FRB domain, : 1 : 41 entities Detail

Interaction partners 1. FRB domain, : 18 interactors Detail

Experiments performed 5 experiments Detail