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BMRB: 6761

2AL3

SOLUTION STRUCTURE AND BACKBONE DYNAMICS OF AN N-TERMINAL UBIQUITIN-LIKE DOMAIN IN THE GLUT4-TETHERING PROTEIN, TUG

Authors

Tettamanzi, C.M., Yu, C., Bogan, J., Hodsdon, M.E.

Assembly

UBL1 domain of TUG

Entity

1. UBL1 domain of TUG (polymer, Thiol state: all free), 90 monomers, 9907.119 Da Detail

MAAPAGGGGS AVSVLAPNGR RHTVKVTPST VLLQVLEDTC RRQDFNPSEY DLKFQRTVLD LSLQWRFANL PNNAKLEMVP VSRSREGPEN

Formula weight

9907.119 Da

Exptl. method

NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.5 %, Completeness (bb): 100.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.5 % (969 of 1025)	94.4 % (506 of 536)	95.9 % (379 of 395)	89.4 % (84 of 94)
Backbone	100.0 % (526 of 526)	100.0 % (179 of 179)	100.0 % (264 of 264)	100.0 % (83 of 83)
Sidechain	90.4 % (527 of 583)	91.6 % (327 of 357)	92.6 % (199 of 215)	9.1 % (1 of 11)
Aromatic	70.4 % (38 of 54)	85.2 % (23 of 27)	57.7 % (15 of 26)	0.0 % (0 of 1)
Methyl	95.0 % (95 of 100)	98.0 % (49 of 50)	92.0 % (46 of 50)

1. TUG-UBL1

MAAPAGGGGS AVSVLAPNGR RHTVKVTPST VLLQVLEDTC RRQDFNPSEY DLKFQRTVLD LSLQWRFANL PNNAKLEMVP VSRSREGPEN

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 7.4 (±0.02)

#	Name	Isotope labeling	Concentration
1	TUG-UBL1	[U-98% 13C; U-98% 15N]	0.75 mM
2	phosphate buffer		20 mM
3	NaCl		20 mM
4	sodium azide		0.05 %
5	leupeptin		10 uM
6	pepstatin		10 uM
7	D20		5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.22 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2AL3, Strand ID: A Detail

Release date

2006-04-26

Citation

Solution structure and backbone dynamics of an N-terminal ubiquitin-like domain in the GLUT4-regulating protein, TUG
Tettamanzi, C.M., Yu, C., Bogan, J., Hodsdon, M.E.
Protein Sci. (2006), 15, 498-508, PubMed 16501224 , DOI 10.1110/ps.051901806 , Abstract

Related entities 1. UBL1 domain of TUG, : 1 : 2 : 1 : 7 entities Detail

Interaction partners 1. UBL1 domain of TUG, : 14 interactors Detail

More details for 14 interactors identified by 6 citations

Experiments performed 12 experiments Detail

1 HNCO