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The response regulator TorI belongs to a new family of atypical excisionase
Authors
Elantak, L., Ansaldi, M., Guerlesquin, F., Mejean, V., Morelli, X.
Assembly
Tor inhibition protein
Entity
1. Tor inhibition protein (polymer, Thiol state: all free), 66 monomers, 7677.802 Da Detail

MQHELQPDSL VDLKFIMADT GFGKTFIYDR IKSGDLPKAK VIHGRARWLY RDHCEFKNKL LSRANG


Formula weight
7677.802 Da
Source organism
Escherichia coli
Exptl. method
NMR
Refine. method
non-bonded interaction for simulated annealing and refinement in an explicit water box.
Data set
assigned_chemical_shifts
Chem. Shift Complete1
Sequence coverage: 97.0 %, Completeness: 72.0 %, Completeness (bb): 81.6 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All72.0 % (582 of 808)90.1 % (383 of 425)43.6 % (137 of 314)89.9 % (62 of 69)
Backbone81.6 % (320 of 392)97.0 % (131 of 135)65.8 % (127 of 193)96.9 % (62 of 64)
Sidechain66.5 % (317 of 477)86.9 % (252 of 290)35.7 % (65 of 182) 0.0 % (0 of 5)
Aromatic40.0 % (32 of 80)80.0 % (32 of 40) 0.0 % (0 of 39) 0.0 % (0 of 1)
Methyl64.1 % (41 of 64)93.8 % (30 of 32)34.4 % (11 of 32)

1. prophage excisionase

MQHELQPDSL VDLKFIMADT GFGKTFIYDR IKSGDLPKAK VIHGRARWLY RDHCEFKNKL LSRANG

Sample #1

Pressure 1 atm, Temperature 278 (±1) K, pH 5.9 (±0.1)


#NameIsotope labelingTypeConcentration
1prophage excisionase[U-95% 13C; U-95% 15N]1.5 mM
2phosphate buffer50 mM
3H2090 %
4D2010 %
Sample #2

Pressure 1 atm, Temperature 278 (±1) K, pH 5.9 (±0.1)


#NameIsotope labelingTypeConcentration
5prophage excisionase1.5 mM
6phosphate buffer50 mM
7H2090 %
8D2010 %
Sample #3

Pressure 1 atm, Temperature 278 (±1) K, pH 5.9 (±0.1)


#NameIsotope labelingTypeConcentration
9prophage excisionase[U-95% 15N]1.5 mM
10phosphate buffer50 mM
11H2090 %
12D2010 %
Sample #4

Pressure 1 atm, Temperature 278 (±1) K, pH 5.9 (±0.1)


#NameIsotope labelingTypeConcentration
13prophage excisionase[U-95% 13C]1.5 mM
14phosphate buffer50 mM
15H2090 %
16D2010 %

Chem. Shift Complete2
Sequence coverage: 97.0 %, Completeness: 71.7 %, Completeness (bb): 80.7 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All71.7 % (1158 of 1616)90.2 % (767 of 850)42.5 % (267 of 628)89.9 % (124 of 138)
Backbone80.7 % (633 of 784)97.0 % (262 of 270)64.0 % (247 of 386)96.9 % (124 of 128)
Sidechain66.4 % (633 of 954)87.1 % (505 of 580)35.2 % (128 of 364) 0.0 % (0 of 10)
Aromatic40.0 % (64 of 160)80.0 % (64 of 80) 0.0 % (0 of 78) 0.0 % (0 of 2)
Methyl61.7 % (79 of 128)93.8 % (60 of 64)29.7 % (19 of 64)

1. prophage excisionase

MQHELQPDSL VDLKFIMADT GFGKTFIYDR IKSGDLPKAK VIHGRARWLY RDHCEFKNKL LSRANG

Sample

Pressure 1 atm, Temperature 278 (±1) K, pH 5.9 (±0.1)


#NameIsotope labelingTypeConcentration
5prophage excisionase1.5 mM
6phosphate buffer50 mM
7H2090 %
8D2010 %

LACS Plot; CA
Referencing offset: 0.3 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: 0.3 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.2 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 17 models in PDB: 1Z4H, Strand ID: A Detail


Release date
2007-02-05
Citation
Structural and genetic analyses reveal a key role in prophage excision for the TorI response regulator inhibitor
Elantak, L., Ansaldi, M., Guerlesquin, F., Mejean, V., Morelli, X.
J. Biol. Chem. (2005), 280, 36802-36808, PubMed 16079126 , DOI 10.1074/jbc.M507409200 ,
Entries sharing articles Swiss-Prot: 1 entries Detail
  Swiss-Prot: Q2EES9 released on 2006-10-03
    Title TORI_ECOLI Entity Response regulator inhibitor for tor operon
Related entities 1. Tor inhibition protein, : 1 : 2 : 10 entities Detail
Experiments performed 10 experiments Detail
nullKeywords winged helix, reverse turn