The response regulator TorI belongs to a new family of atypical excisionase
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 72.0 % (582 of 808) | 90.1 % (383 of 425) | 43.6 % (137 of 314) | 89.9 % (62 of 69) |
Backbone | 81.6 % (320 of 392) | 97.0 % (131 of 135) | 65.8 % (127 of 193) | 96.9 % (62 of 64) |
Sidechain | 66.5 % (317 of 477) | 86.9 % (252 of 290) | 35.7 % (65 of 182) | 0.0 % (0 of 5) |
Aromatic | 40.0 % (32 of 80) | 80.0 % (32 of 40) | 0.0 % (0 of 39) | 0.0 % (0 of 1) |
Methyl | 64.1 % (41 of 64) | 93.8 % (30 of 32) | 34.4 % (11 of 32) |
1. prophage excisionase
MQHELQPDSL VDLKFIMADT GFGKTFIYDR IKSGDLPKAK VIHGRARWLY RDHCEFKNKL LSRANGPressure 1 atm, Temperature 278 (±1) K, pH 5.9 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | prophage excisionase | [U-95% 13C; U-95% 15N] | 1.5 mM | |
2 | phosphate buffer | 50 mM | ||
3 | H20 | 90 % | ||
4 | D20 | 10 % |
Pressure 1 atm, Temperature 278 (±1) K, pH 5.9 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | prophage excisionase | 1.5 mM | ||
6 | phosphate buffer | 50 mM | ||
7 | H20 | 90 % | ||
8 | D20 | 10 % |
Pressure 1 atm, Temperature 278 (±1) K, pH 5.9 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | prophage excisionase | [U-95% 15N] | 1.5 mM | |
10 | phosphate buffer | 50 mM | ||
11 | H20 | 90 % | ||
12 | D20 | 10 % |
Pressure 1 atm, Temperature 278 (±1) K, pH 5.9 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | prophage excisionase | [U-95% 13C] | 1.5 mM | |
14 | phosphate buffer | 50 mM | ||
15 | H20 | 90 % | ||
16 | D20 | 10 % |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 71.7 % (1158 of 1616) | 90.2 % (767 of 850) | 42.5 % (267 of 628) | 89.9 % (124 of 138) |
Backbone | 80.7 % (633 of 784) | 97.0 % (262 of 270) | 64.0 % (247 of 386) | 96.9 % (124 of 128) |
Sidechain | 66.4 % (633 of 954) | 87.1 % (505 of 580) | 35.2 % (128 of 364) | 0.0 % (0 of 10) |
Aromatic | 40.0 % (64 of 160) | 80.0 % (64 of 80) | 0.0 % (0 of 78) | 0.0 % (0 of 2) |
Methyl | 61.7 % (79 of 128) | 93.8 % (60 of 64) | 29.7 % (19 of 64) |
1. prophage excisionase
MQHELQPDSL VDLKFIMADT GFGKTFIYDR IKSGDLPKAK VIHGRARWLY RDHCEFKNKL LSRANGPressure 1 atm, Temperature 278 (±1) K, pH 5.9 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | prophage excisionase | 1.5 mM | ||
6 | phosphate buffer | 50 mM | ||
7 | H20 | 90 % | ||
8 | D20 | 10 % |