BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	90.7 % (708 of 781)	97.8 % (400 of 409)	79.0 % (237 of 300)	98.6 % (71 of 72)
Backbone	84.2 % (330 of 392)	96.3 % (129 of 134)	71.1 % (138 of 194)	98.4 % (63 of 64)
Sidechain	97.6 % (440 of 451)	98.5 % (271 of 275)	95.8 % (161 of 168)	100.0 % (8 of 8)
Aromatic	86.5 % (45 of 52)	100.0 % (26 of 26)	72.0 % (18 of 25)	100.0 % (1 of 1)
Methyl	98.6 % (69 of 70)	97.1 % (34 of 35)	100.0 % (35 of 35)

1. Regulatory protein cro

MEQRITLKDY AMRFGQTKTA KDLGVQQSAI NKWIHAGRKI FLTINADGSV YAEEVKPDPS NKKTTA

Show sample condition

Sample

Pressure 1 atm, Temperature 293 K, pH 5.3

#	Name	Isotope labeling	Concentration
1	Regulatory protein cro	[U-13C]	2.5 mM
2	Na-phosphate		50 mM
3	sodium azide		0.01 %
4	TSP		1 mM
5	H2O		90 %
6	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: -0.24 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: -0.24 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: -0.14 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2A63, Strand ID: A Detail

Release date

2006-06-25

Citation

A trade between similar but nonequivalent intrasubunit and intersubunit contacts in Cro dimer evolution
Newlove, T., Atkinson, K.R., Van Dorn, L.O., Cordes, M.H.
Biochemistry (2006), 45, 6379-6391, PubMed 16700549 , DOI 10.1021/bi052541c , Abstract

Related entities 1. Regulatory protein cro, : 1 : 6 : 8 entities Detail

Interaction partners 1. Regulatory protein cro, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 6 experiments Detail

1 3D 15N-separated NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm

#	Name	Isotope labeling	Concentration
1	Regulatory protein cro	[U-13C]	2.5 mM
2	Na-phosphate		50 mM
3	sodium azide		0.01 %
4	TSP		1 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm

#	Name	Isotope labeling	Concentration
7	Regulatory protein cro	[U-15N]	5 mM
8	Na-phosphate		50 mM
9	sodium azide		0.01 %
10	TSP		1 mM
11	H2O		90 %
12	D2O		10 %

Sample #3

Pressure 1 atm

#	Name	Concentration
13	Regulatory protein cro	5 mM
14	Na-phosphate	50 mM
15	sodium azide	0.01 %
16	TSP	1 mM
17	H2O	90 %
18	D2O	10 %

Sample #4

Pressure 1 atm

#	Name	Isotope labeling	Concentration
19	Regulatory protein cro	[U-15N]	5 mM
20	Na-phosphate		50 mM
21	sodium azide		0.01 %
22	TSP		1 mM
23	D2O		100 %

2 HNHA

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm

#	Name	Isotope labeling	Concentration
1	Regulatory protein cro	[U-13C]	2.5 mM
2	Na-phosphate		50 mM
3	sodium azide		0.01 %
4	TSP		1 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm

#	Name	Isotope labeling	Concentration
7	Regulatory protein cro	[U-15N]	5 mM
8	Na-phosphate		50 mM
9	sodium azide		0.01 %
10	TSP		1 mM
11	H2O		90 %
12	D2O		10 %

Sample #3

Pressure 1 atm

#	Name	Concentration
13	Regulatory protein cro	5 mM
14	Na-phosphate	50 mM
15	sodium azide	0.01 %
16	TSP	1 mM
17	H2O	90 %
18	D2O	10 %

Sample #4

Pressure 1 atm

#	Name	Isotope labeling	Concentration
19	Regulatory protein cro	[U-15N]	5 mM
20	Na-phosphate		50 mM
21	sodium azide		0.01 %
22	TSP		1 mM
23	D2O		100 %

3 HNHB

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm

#	Name	Isotope labeling	Concentration
1	Regulatory protein cro	[U-13C]	2.5 mM
2	Na-phosphate		50 mM
3	sodium azide		0.01 %
4	TSP		1 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm

#	Name	Isotope labeling	Concentration
7	Regulatory protein cro	[U-15N]	5 mM
8	Na-phosphate		50 mM
9	sodium azide		0.01 %
10	TSP		1 mM
11	H2O		90 %
12	D2O		10 %

Sample #3

Pressure 1 atm

#	Name	Concentration
13	Regulatory protein cro	5 mM
14	Na-phosphate	50 mM
15	sodium azide	0.01 %
16	TSP	1 mM
17	H2O	90 %
18	D2O	10 %

Sample #4

Pressure 1 atm

#	Name	Isotope labeling	Concentration
19	Regulatory protein cro	[U-15N]	5 mM
20	Na-phosphate		50 mM
21	sodium azide		0.01 %
22	TSP		1 mM
23	D2O		100 %

4 HSQC (amide hydrogen exchange)

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm

#	Name	Isotope labeling	Concentration
1	Regulatory protein cro	[U-13C]	2.5 mM
2	Na-phosphate		50 mM
3	sodium azide		0.01 %
4	TSP		1 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm

#	Name	Isotope labeling	Concentration
7	Regulatory protein cro	[U-15N]	5 mM
8	Na-phosphate		50 mM
9	sodium azide		0.01 %
10	TSP		1 mM
11	H2O		90 %
12	D2O		10 %

Sample #3

Pressure 1 atm

#	Name	Concentration
13	Regulatory protein cro	5 mM
14	Na-phosphate	50 mM
15	sodium azide	0.01 %
16	TSP	1 mM
17	H2O	90 %
18	D2O	10 %

Sample #4

Pressure 1 atm

#	Name	Isotope labeling	Concentration
19	Regulatory protein cro	[U-15N]	5 mM
20	Na-phosphate		50 mM
21	sodium azide		0.01 %
22	TSP		1 mM
23	D2O		100 %

5 3D 13C-separated NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm

#	Name	Isotope labeling	Concentration
1	Regulatory protein cro	[U-13C]	2.5 mM
2	Na-phosphate		50 mM
3	sodium azide		0.01 %
4	TSP		1 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm

#	Name	Isotope labeling	Concentration
7	Regulatory protein cro	[U-15N]	5 mM
8	Na-phosphate		50 mM
9	sodium azide		0.01 %
10	TSP		1 mM
11	H2O		90 %
12	D2O		10 %

Sample #3

Pressure 1 atm

#	Name	Concentration
13	Regulatory protein cro	5 mM
14	Na-phosphate	50 mM
15	sodium azide	0.01 %
16	TSP	1 mM
17	H2O	90 %
18	D2O	10 %

Sample #4

Pressure 1 atm

#	Name	Isotope labeling	Concentration
19	Regulatory protein cro	[U-15N]	5 mM
20	Na-phosphate		50 mM
21	sodium azide		0.01 %
22	TSP		1 mM
23	D2O		100 %

6 2D NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm

#	Name	Isotope labeling	Concentration
1	Regulatory protein cro	[U-13C]	2.5 mM
2	Na-phosphate		50 mM
3	sodium azide		0.01 %
4	TSP		1 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm

#	Name	Isotope labeling	Concentration
7	Regulatory protein cro	[U-15N]	5 mM
8	Na-phosphate		50 mM
9	sodium azide		0.01 %
10	TSP		1 mM
11	H2O		90 %
12	D2O		10 %

Sample #3

Pressure 1 atm

#	Name	Concentration
13	Regulatory protein cro	5 mM
14	Na-phosphate	50 mM
15	sodium azide	0.01 %
16	TSP	1 mM
17	H2O	90 %
18	D2O	10 %

Sample #4

Pressure 1 atm

#	Name	Isotope labeling	Concentration
19	Regulatory protein cro	[U-15N]	5 mM
20	Na-phosphate		50 mM
21	sodium azide		0.01 %
22	TSP		1 mM
23	D2O		100 %

nullKeywords ball-and-socket, helix-turn-helix, monomer

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Found 1 Document (1.517 seconds)

BMRB: 6771

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Regulatory protein cro, : 1 : 6 : 8 entities Detail

Interaction partners 1. Regulatory protein cro, : 1 interactors Detail

Experiments performed 6 experiments Detail

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