BMRB: 6780

NMR Assignment of HI1506, a novel two-domain protein from Haemophilus influenzae

Authors

Sari, N., Doseeva, V., Subarian, K., Herzberg, O., Orban, J.

Assembly

hi1506

Entity

1. hi1506 (polymer, Thiol state: all free), 131 monomers, 14122.54 Da Detail

GSHMDKTFCV VVQNRIKEGY RRAGFSFHLG DNSLAAVSES QLAQLKADPR LVVQITETGS QEGGEGLSKE PAGSDEQKQL RADPPSTDLN TFTVEQLKAQ LTERGITFKQ SATKAELIAL FAPADGEKSE A

Formula weight

14122.54 Da

Source organism

Haemophilus influenzae

Exptl. method

NMR

Refine. method

structures were calculated using standard simulated annealing and torsion angle dynamics protocols in CNS version 1.1

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.5 %, Completeness: 89.8 %, Completeness (bb): 93.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	89.8 % (1318 of 1467)	87.2 % (666 of 764)	92.7 % (523 of 564)	92.8 % (129 of 139)
Backbone	93.8 % (728 of 776)	93.7 % (251 of 268)	93.5 % (357 of 382)	95.2 % (120 of 126)
Sidechain	87.2 % (707 of 811)	83.7 % (415 of 496)	93.7 % (283 of 302)	69.2 % (9 of 13)
Aromatic	80.3 % (61 of 76)	78.9 % (30 of 38)	81.6 % (31 of 38)
Methyl	97.1 % (134 of 138)	94.2 % (65 of 69)	100.0 % (69 of 69)

1. hi1506

GSHMDKTFCV VVQNRIKEGY RRAGFSFHLG DNSLAAVSES QLAQLKADPR LVVQITETGS QEGGEGLSKE PAGSDEQKQL RADPPSTDLN TFTVEQLKAQ LTERGITFKQ SATKAELIAL FAPADGEKSE A

Show sample condition

Sample

Pressure 760 (±0) mmHg, Temperature 298 (±0.1) K, pH 7 (±0)

#	Name	Isotope labeling	Type	Concentration
1	hi1506	[U-13C; U-15N]		1.0 (±0.1) mM
2	potassium phosphate			50 mM
3	NaCl			100 mM
4	DTT			1 mM
5	D2O			90 %
6	H2O			10 %

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LACS Plot; CA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.04 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2OUT, Strand ID: A Detail

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Release date

2005-12-19

Citation

NMR assignment of HI1506, a novel two-domain protein from Haemophilus influenzae
Sari, N., Yeh, D.C., Doseeva, V., Surabian, K., Herzberg, O., Orban, J.
J. Biomol. NMR (2005), 33, 281-281, PubMed 16341755 , DOI 10.1007/s10858-005-3869-3 , Abstract

Related entities 1. hi1506, : 1 : 1 entities Detail

Experiments performed 12 experiments Detail