BMRB: 6781

Resonance Assignments for the ADA2-like Swirm domain

Authors

Qian, C., Zeng, L., Zhang, Q., Zhou, M.

Assembly

ada2 swirm domain polypeptide

Entity

1. ada2 swirm domain polypeptide (polymer, Thiol state: not present), 90 monomers, 10025.53 Da Detail

GSNSGRRSAP PLNLTGLPGT EKLNEKEKEL CQVVRLVPGA YLEYKSALLN ECHKQGGLRL AQARALIKID VNKTRKIYDF LIREGYITKA

Formula weight

10025.53 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

simulated annealing torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.8 %, Completeness: 79.0 %, Completeness (bb): 82.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	79.0 % (843 of 1067)	82.2 % (463 of 563)	72.0 % (295 of 410)	90.4 % (85 of 94)
Backbone	82.5 % (439 of 532)	95.1 % (175 of 184)	68.7 % (180 of 262)	97.7 % (84 of 86)
Sidechain	78.6 % (485 of 617)	76.0 % (288 of 379)	85.2 % (196 of 230)	12.5 % (1 of 8)
Aromatic	52.2 % (24 of 46)	52.2 % (12 of 23)	52.2 % (12 of 23)
Methyl	96.4 % (106 of 110)	96.4 % (53 of 55)	96.4 % (53 of 55)

1. ADA2 swirm domain

GSNSGRRSAP PLNLTGLPGT EKLNEKEKEL CQVVRLVPGA YLEYKSALLN ECHKQGGLRL AQARALIKID VNKTRKIYDF LIREGYITKA

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Sample

Temperature 278 (±0.1) K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Ada2 swirm domain	[U-13C; U-15N]		0.5 mM
2	NaPi			50 mM
3	NaCl			100 mM
4	DTT			2 mM
5	EDTA			0.02 mM

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LACS Plot; CA

Referencing offset: -0.44 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.44 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 1 models in PDB: 2AQF, Strand ID: A Detail

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Release date

2006-09-10

Citation

Structure and chromosomal DNA binding of the SWIRM domain
Qian, C., Zhang, Q., Li, S., Zeng, L., Walsh, M.J., Zhou, M.
Nat. Struct. Mol. Biol. (2005), 12, 1078-1085, PubMed 16299514 , DOI 10.1038/nsmb1022 , Abstract

Related entities 1. ada2 swirm domain polypeptide, : 1 : 1 : 5 : 52 entities Detail

Interaction partners 1. ada2 swirm domain polypeptide, : 7 interactors Detail

Experiments performed 8 experiments Detail