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BMRB: 6797

2PXG

NMR assignment of the outer membrane lipoprotein (OmlA) from Xanthomonas axonopodis pv citri

Authors

Vanini, M.M. T., Benedetti, C.E., Spisni, A., Pertinhez, T.A.

Assembly

OmlA

Entity

1. OmlA (polymer, Thiol state: not present), 118 monomers, 13583.13 Da Detail

MAGSGIIYKQ PIYQGNLIKQ NAVEQLQVGQ SKQQVSALLG TPSIPDPFHA QRWDYTSTQR VDRLARTEIK NFTVFFENEQ VVRWEGDYFP SQDEQLAKAA PKQFGRNLAR DKKKQRGR

Formula weight

13583.13 Da

Source organism

Xanthomonas citri

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.5 %, Completeness (bb): 99.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.5 % (1389 of 1439)	96.5 % (736 of 763)	95.9 % (520 of 542)	99.3 % (133 of 134)
Backbone	99.1 % (690 of 696)	99.6 % (237 of 238)	98.8 % (342 of 346)	99.1 % (111 of 112)
Sidechain	94.5 % (806 of 853)	95.0 % (499 of 525)	93.1 % (285 of 306)	100.0 % (22 of 22)
Aromatic	86.7 % (104 of 120)	88.3 % (53 of 60)	84.5 % (49 of 58)	100.0 % (2 of 2)
Methyl	96.3 % (104 of 108)	96.3 % (52 of 54)	96.3 % (52 of 54)

1. OmlA

MAGSGIIYKQ PIYQGNLIKQ NAVEQLQVGQ SKQQVSALLG TPSIPDPFHA QRWDYTSTQR VDRLARTEIK NFTVFFENEQ VVRWEGDYFP SQDEQLAKAA PKQFGRNLAR DKKKQRGR

Show sample condition

Sample

Temperature 298 K, pH 5.8 (±0.1), Details 0.3 mM OmlA, 100 mM NaH2PO4 pH 5.8 , 0.05% nAn3, 5% D2O

#	Name	Isotope labeling	Concentration
1	OmlA	[U-15N; U-13C]	0.3 mM
2	NaH2PO4		100 mM
3	nAn3		0.05 %
4	D2O		5 %
5	H2O		95 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.58 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.58 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 6 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2PXG, Strand ID: A Detail

Release date

2006-04-10

Citation

NMR assignment of the outer membrane lipoprotein (OmlA) from Xanthomonas axonopodis pv citri
Vanini, M.M. T., Benedetti, C.E., Spisni, A., Pertinhez, T.A.
J. Biomol. NMR (2006), 36 Suppl 1, 34-34, PubMed 16596342 , DOI 10.1007/s10858-006-0022-x , Abstract

Related entities 1. OmlA, : 1 : 3 entities Detail

Experiments performed 10 experiments Detail

1 1H15N13CA