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BMRB: 6801

2AWT

1H, 13C, and 15N Chemical Shift Assignments for Human Small Ubiquitin-like Modifier Protein Isoform 2 (SUMO-2)

Authors

Chang, C., Wang, Y., Chung, T., Chang, C., Li, S.S.-L., Huang, T.

Assembly

Human SUMO-2 monomer

Entity

1. Human SUMO-2 monomer (polymer, Thiol state: all free), 96 monomers, 11034.27 Da Detail

MADEKPKEGV KTENNDHINL KVAGQDGSVV QFKIKRHTPL SKLMKAYCER QGLSMRQIRF RFDGQPINET DTPAQLEMED EDTIDVFQQQ TGGVYY

Formula weight

11034.27 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.9 %, Completeness: 86.5 %, Completeness (bb): 94.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.5 % (985 of 1139)	86.4 % (520 of 602)	85.6 % (370 of 432)	90.5 % (95 of 105)
Backbone	94.0 % (534 of 568)	94.9 % (185 of 195)	92.9 % (261 of 281)	95.7 % (88 of 92)
Sidechain	81.2 % (536 of 660)	82.3 % (335 of 407)	80.8 % (194 of 240)	53.8 % (7 of 13)
Aromatic	37.5 % (27 of 72)	66.7 % (24 of 36)	8.3 % (3 of 36)
Methyl	97.6 % (82 of 84)	97.6 % (41 of 42)	97.6 % (41 of 42)

1. human SUMO-2

MADEKPKEGV KTENNDHINL KVAGQDGSVV QFKIKRHTPL SKLMKAYCER QGLSMRQIRF RFDGQPINET DTPAQLEMED EDTIDVFQQQ TGGVYY

Show sample condition

Sample

Temperature 303 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	human SUMO-2	[U-95% 13C; U-95% 15N]	protein	0.8 (±0.1) mM
2	sodium phoshate		buffer	50 (±1.0) mM
3	sodium chloride		salt	150 (±1.0) mM
4	EDTA		salt	1 (±0.01) mM
5	DSS		salt	1 (±0.01) mM
6	deuterium oxide		solvent	10 (±1.0) %
7	water		solvent	90 (±1.0) %
8	sodium azide		additive	0.01 (±1.0E-4) %
9	Complete(R) protease inhibitor cocktail		additive

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.58 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.58 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 4 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -2.82 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2AWT, Strand ID: A Detail

Release date

2007-04-10

Citation

Solution structure and dynamics of human SUMO-2
Chang, C., Wang, Y., Chang, C., Chung, T., Tien, S., Li, S.S.-L., Huang, T.
J. Mol. Biol.

Related entities 1. Human SUMO-2 monomer, : 1 : 35 : 148 entities Detail

Interaction partners 1. Human SUMO-2 monomer, : 34 interactors Detail

More details for 34 interactors identified by 18 citations

Experiments performed 12 experiments Detail

1 1H15N_HSQC