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Found 1 Document (1.222 seconds)

BMRB: 6803

2D1U

1H, 13C, and 15N Chemical Shift Assignments for the periplasmic signaling domain of FecA from Escherichia coli

Authors

Garcia-Herrero, A., Vogel, H.J.

Assembly

FecA N-terminal periplasmic signaling domain

Entity

1. FecA N-terminal periplasmic signaling domain (polymer, Thiol state: not present), 104 monomers, 11137.98 Da Detail

AQVNIAPGSL DKALNQYAAH SGFTLSVDAS LTRGKQSNGL HGDYDVESGL QQLLDGSGLQ VKPLGNNSWT LEPAPAPKED ALTVVGDWLG DARENDLEHH HHHH

Formula weight

11137.98 Da

Source organism

Escherichia coli

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.1 %, Completeness: 91.3 %, Completeness (bb): 93.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.3 % (1034 of 1133)	93.6 % (545 of 582)	87.7 % (384 of 438)	92.9 % (105 of 113)
Backbone	93.2 % (572 of 614)	92.1 % (197 of 214)	94.4 % (284 of 301)	91.9 % (91 of 99)
Sidechain	89.2 % (546 of 612)	93.8 % (345 of 368)	81.3 % (187 of 230)	100.0 % (14 of 14)
Aromatic	37.8 % (31 of 82)	70.7 % (29 of 41)	0.0 % (0 of 39)	100.0 % (2 of 2)
Methyl	100.0 % (112 of 112)	100.0 % (56 of 56)	100.0 % (56 of 56)

1. NTFecA

AQVNIAPGSL DKALNQYAAH SGFTLSVDAS LTRGKQSNGL HGDYDVESGL QQLLDGSGLQ VKPLGNNSWT LEPAPAPKED ALTVVGDWLG DARENDLEHH HHHH

Show sample condition

Sample #1

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
1	NTFecA	[U-15N]	protein	1 mM
2	Sodium phosphate		buffer	50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
3	NTFecA	[U-15N; U-13C]	protein	1 mM
4	Sodium Phosphate		buffer	50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2D1U, Strand ID: A Detail

Release date

2005-12-21

Citation

Nuclear magnetic resonance solution structure of the periplasmic signalling domain of the TonB-dependent outer membrane transporter FecA from Escherichia coli
Garcia-Herrero, A., Vogel, H.J.
Mol. Microbiol. (2005), 58, 1226-1237, PubMed 16313612 , DOI 10.1111/j.1365-2958.2005.04889.x , Abstract

Related entities 1. FecA N-terminal periplasmic signaling domain, : 1 : 15 entities Detail

Interaction partners 1. FecA N-terminal periplasmic signaling domain, : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 16 experiments Detail

1 1D Proton

Sample #1

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
1	NTFecA	[U-15N]	protein	1 mM
2	Sodium phosphate		buffer	50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
3	NTFecA	[U-15N; U-13C]	protein	1 mM
4	Sodium Phosphate		buffer	50 mM

2 2D Proton-Proton NOESY (D2O)

Sample #1

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
1	NTFecA	[U-15N]	protein	1 mM
2	Sodium phosphate		buffer	50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
3	NTFecA	[U-15N; U-13C]	protein	1 mM
4	Sodium Phosphate		buffer	50 mM

3 2D 1H-15N HSQC

Sample #1

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
1	NTFecA	[U-15N]	protein	1 mM
2	Sodium phosphate		buffer	50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
3	NTFecA	[U-15N; U-13C]	protein	1 mM
4	Sodium Phosphate		buffer	50 mM

4 HNCO

Sample #1

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
1	NTFecA	[U-15N]	protein	1 mM
2	Sodium phosphate		buffer	50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
3	NTFecA	[U-15N; U-13C]	protein	1 mM
4	Sodium Phosphate		buffer	50 mM

5 HN(CA)CO

Sample #1

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
1	NTFecA	[U-15N]	protein	1 mM
2	Sodium phosphate		buffer	50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
3	NTFecA	[U-15N; U-13C]	protein	1 mM
4	Sodium Phosphate		buffer	50 mM

6 CBCA(CO)NH

Sample #1

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
1	NTFecA	[U-15N]	protein	1 mM
2	Sodium phosphate		buffer	50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
3	NTFecA	[U-15N; U-13C]	protein	1 mM
4	Sodium Phosphate		buffer	50 mM

7 CBCANH

Sample #1

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
1	NTFecA	[U-15N]	protein	1 mM
2	Sodium phosphate		buffer	50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
3	NTFecA	[U-15N; U-13C]	protein	1 mM
4	Sodium Phosphate		buffer	50 mM

8 HCCH-TOCSY

Sample #1

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
1	NTFecA	[U-15N]	protein	1 mM
2	Sodium phosphate		buffer	50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
3	NTFecA	[U-15N; U-13C]	protein	1 mM
4	Sodium Phosphate		buffer	50 mM

9 CCCONH-TOCSY

Sample #1

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
1	NTFecA	[U-15N]	protein	1 mM
2	Sodium phosphate		buffer	50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
3	NTFecA	[U-15N; U-13C]	protein	1 mM
4	Sodium Phosphate		buffer	50 mM

10 HBHA(CO)NH

Sample #1

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
1	NTFecA	[U-15N]	protein	1 mM
2	Sodium phosphate		buffer	50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
3	NTFecA	[U-15N; U-13C]	protein	1 mM
4	Sodium Phosphate		buffer	50 mM

11 HCCCONH-TOCSY

Sample #1

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
1	NTFecA	[U-15N]	protein	1 mM
2	Sodium phosphate		buffer	50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
3	NTFecA	[U-15N; U-13C]	protein	1 mM
4	Sodium Phosphate		buffer	50 mM

12 3D 13C separated NOESY

Sample #1

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
1	NTFecA	[U-15N]	protein	1 mM
2	Sodium phosphate		buffer	50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
3	NTFecA	[U-15N; U-13C]	protein	1 mM
4	Sodium Phosphate		buffer	50 mM

13 3D 15N separated NOESY

Sample #1

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
1	NTFecA	[U-15N]	protein	1 mM
2	Sodium phosphate		buffer	50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
3	NTFecA	[U-15N; U-13C]	protein	1 mM
4	Sodium Phosphate		buffer	50 mM

14 Steady State 15N Heteronuclear noe

Sample #1

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
1	NTFecA	[U-15N]	protein	1 mM
2	Sodium phosphate		buffer	50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
3	NTFecA	[U-15N; U-13C]	protein	1 mM
4	Sodium Phosphate		buffer	50 mM

15 T2 15N

Sample #1

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
1	NTFecA	[U-15N]	protein	1 mM
2	Sodium phosphate		buffer	50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
3	NTFecA	[U-15N; U-13C]	protein	1 mM
4	Sodium Phosphate		buffer	50 mM

16 T2 15N

Sample #1

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
1	NTFecA	[U-15N]	protein	1 mM
2	Sodium phosphate		buffer	50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6 (±0.01)

#	Name	Isotope labeling	Type	Concentration
3	NTFecA	[U-15N; U-13C]	protein	1 mM
4	Sodium Phosphate		buffer	50 mM

nullKeywords FecA, iron-uptake, surface signaling, iron uptake, Surface signaling, Iron-uptake

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Found 1 Document (1.222 seconds)

BMRB: 6803

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. FecA N-terminal periplasmic signaling domain, : 1 : 15 entities Detail

Interaction partners 1. FecA N-terminal periplasmic signaling domain, : 2 interactors Detail

Experiments performed 16 experiments Detail

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