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Found 1 Document (1.679 seconds)

BMRB: 6804

2B88

Letter to the Editor:NMR assignments of the free and bound-state components of an anti-idiotypic affibody complex

Authors

Dogan, J., Lendel, C., Hard, T.

Assembly

Ztaq

Entity

1. Ztaq (polymer, Thiol state: not present), 58 monomers, 6484.197 Da Detail

VDNKFNKELG WATWEIFNLP NLNGVQVKAF IDSLRDDPSQ SANLLAEAKK LNDAQAPK

Formula weight

6484.197 Da

Exptl. method

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.1 %, Completeness (bb): 98.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.1 % (651 of 692)	96.4 % (349 of 362)	90.1 % (237 of 263)	97.0 % (65 of 67)
Backbone	98.0 % (335 of 342)	96.5 % (111 of 115)	99.4 % (171 of 172)	96.4 % (53 of 55)
Sidechain	91.6 % (372 of 406)	96.4 % (238 of 247)	83.0 % (122 of 147)	100.0 % (12 of 12)
Aromatic	44.4 % (24 of 54)	81.5 % (22 of 27)	0.0 % (0 of 25)	100.0 % (2 of 2)
Methyl	100.0 % (64 of 64)	100.0 % (32 of 32)	100.0 % (32 of 32)

1. Ztaq

VDNKFNKELG WATWEIFNLP NLNGVQVKAF IDSLRDDPSQ SANLLAEAKK LNDAQAPK

Show sample condition

Sample

Pressure 1 (±0) atm, Temperature 298 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	Ztaq	[U-13C; U-15N]	protein	1.2 mM
2	Potassium Phosphate		buffer	20 mM
3	Sodium Chloride		salt	50 mM
4	Sodium Azide			0.01 %
5	D2O			10 %

Protein Blocks Logo

Calculated from 40 models in PDB: 2B88, Strand ID: A Detail

Release date

2006-03-09

Citation

NMR assignments of the free and bound-state protein components of an anti-idiotypic affibody complex
Dogan, J., Lendel, C., Hard, T.
J. Biomol. NMR (2006), 36 Suppl 1, 13-13, PubMed 16456703 , DOI 10.1007/s10858-005-5350-8 , Abstract

Related entities 1. Ztaq, : 1 : 1 : 2 : 162 entities Detail

Experiments performed 12 experiments Detail

1 1H-15N HSQC

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	Ztaq	[U-13C; U-15N]	protein	1.2 mM
2	Potassium Phosphate		buffer	20 mM
3	Sodium Chloride		salt	50 mM
4	Sodium Azide			0.01 %
5	D2O			10 %

2 1H-13C HSQC

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	Ztaq	[U-13C; U-15N]	protein	1.2 mM
2	Potassium Phosphate		buffer	20 mM
3	Sodium Chloride		salt	50 mM
4	Sodium Azide			0.01 %
5	D2O			10 %

3 CBCA(CO)NH

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	Ztaq	[U-13C; U-15N]	protein	1.2 mM
2	Potassium Phosphate		buffer	20 mM
3	Sodium Chloride		salt	50 mM
4	Sodium Azide			0.01 %
5	D2O			10 %

4 CBCANH

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	Ztaq	[U-13C; U-15N]	protein	1.2 mM
2	Potassium Phosphate		buffer	20 mM
3	Sodium Chloride		salt	50 mM
4	Sodium Azide			0.01 %
5	D2O			10 %

5 HNCO

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	Ztaq	[U-13C; U-15N]	protein	1.2 mM
2	Potassium Phosphate		buffer	20 mM
3	Sodium Chloride		salt	50 mM
4	Sodium Azide			0.01 %
5	D2O			10 %

6 HN(CA)CO

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	Ztaq	[U-13C; U-15N]	protein	1.2 mM
2	Potassium Phosphate		buffer	20 mM
3	Sodium Chloride		salt	50 mM
4	Sodium Azide			0.01 %
5	D2O			10 %

7 HCCH-COSY

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	Ztaq	[U-13C; U-15N]	protein	1.2 mM
2	Potassium Phosphate		buffer	20 mM
3	Sodium Chloride		salt	50 mM
4	Sodium Azide			0.01 %
5	D2O			10 %

8 HCCH-TOCSY

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	Ztaq	[U-13C; U-15N]	protein	1.2 mM
2	Potassium Phosphate		buffer	20 mM
3	Sodium Chloride		salt	50 mM
4	Sodium Azide			0.01 %
5	D2O			10 %

9 (H)CC(CO)NH

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	Ztaq	[U-13C; U-15N]	protein	1.2 mM
2	Potassium Phosphate		buffer	20 mM
3	Sodium Chloride		salt	50 mM
4	Sodium Azide			0.01 %
5	D2O			10 %

10 15N-NOESY HSQC

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	Ztaq	[U-13C; U-15N]	protein	1.2 mM
2	Potassium Phosphate		buffer	20 mM
3	Sodium Chloride		salt	50 mM
4	Sodium Azide			0.01 %
5	D2O			10 %

11 15N-TOCSY HSQC

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	Ztaq	[U-13C; U-15N]	protein	1.2 mM
2	Potassium Phosphate		buffer	20 mM
3	Sodium Chloride		salt	50 mM
4	Sodium Azide			0.01 %
5	D2O			10 %

12 13C-NOESY HSQC

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	Ztaq	[U-13C; U-15N]	protein	1.2 mM
2	Potassium Phosphate		buffer	20 mM
3	Sodium Chloride		salt	50 mM
4	Sodium Azide			0.01 %
5	D2O			10 %

nullKeywords affibody