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Found 1 Document (1.611 seconds)

BMRB: 6805

2B89

Letter to the Editor: NMR assignments of the free and bound-state components of an anti-idiotypic affibody complex

Authors

Dogan, J., Lendel, C., Hard, T.

Assembly

anti-Ztaq

Entity

1. anti-Ztaq (polymer, Thiol state: not present), 58 monomers, 6419.281 Da Detail

VDNKFNKERV IAIGEIMRLP NLNSLQVVAF INSLRDDPSQ SANLLAEAKK LNDAQAPK

Formula weight

6419.281 Da

Exptl. method

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.9 %, Completeness (bb): 97.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.9 % (663 of 684)	98.1 % (353 of 360)	95.4 % (247 of 259)	96.9 % (63 of 65)
Backbone	97.7 % (334 of 342)	96.5 % (110 of 114)	98.8 % (171 of 173)	96.4 % (53 of 55)
Sidechain	96.7 % (386 of 399)	98.8 % (243 of 246)	93.0 % (133 of 143)	100.0 % (10 of 10)
Aromatic	50.0 % (10 of 20)	100.0 % (10 of 10)	0.0 % (0 of 10)
Methyl	100.0 % (74 of 74)	100.0 % (37 of 37)	100.0 % (37 of 37)

1. anti-Ztaq

VDNKFNKERV IAIGEIMRLP NLNSLQVVAF INSLRDDPSQ SANLLAEAKK LNDAQAPK

Show sample condition

Sample

Pressure 1 (±0) atm, Temperature 298 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	anti-Ztaq	[U-13C; U-15N]	protein	1.5 mM
2	Potassium Phosphate		buffer	20 mM
3	Sodium Chloride		salt	50 mM
4	Sodium Azide			0.01 %
5	D2O			10 % v/v

Protein Blocks Logo

Calculated from 40 models in PDB: 2B89, Strand ID: A Detail

Release date

2006-03-09

Citation

NMR assignments of the free and bound-state protein components of an anti-idiotypic affibody complex
Dogan, J., Lendel, C., Hard, T.
J. Biomol. NMR (2006), 36 Suppl 1, 13-13, PubMed 16456703 , DOI 10.1007/s10858-005-5350-8 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 6804 released on 2006-03-09
    Title Letter to the Editor:NMR assignments of the free and bound-state components of an anti-idiotypic affibody complex
  BMRB: 6806 released on 2006-03-09
    Title Letter to the Editor: NMR assignments of the free and bound-state components of an anti-idiotypic affibody complex

Related entities 1. anti-Ztaq, : 1 : 1 : 158 entities Detail

Experiments performed 12 experiments Detail

1 1H-15N HSQC

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	anti-Ztaq	[U-13C; U-15N]	protein	1.5 mM
2	Potassium Phosphate		buffer	20 mM
3	Sodium Chloride		salt	50 mM
4	Sodium Azide			0.01 %
5	D2O			10 % v/v

2 1H-13C HSQC

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	anti-Ztaq	[U-13C; U-15N]	protein	1.5 mM
2	Potassium Phosphate		buffer	20 mM
3	Sodium Chloride		salt	50 mM
4	Sodium Azide			0.01 %
5	D2O			10 % v/v

3 CBCA(CO)NH

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	anti-Ztaq	[U-13C; U-15N]	protein	1.5 mM
2	Potassium Phosphate		buffer	20 mM
3	Sodium Chloride		salt	50 mM
4	Sodium Azide			0.01 %
5	D2O			10 % v/v

4 CBCANH

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	anti-Ztaq	[U-13C; U-15N]	protein	1.5 mM
2	Potassium Phosphate		buffer	20 mM
3	Sodium Chloride		salt	50 mM
4	Sodium Azide			0.01 %
5	D2O			10 % v/v

5 HNCO

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	anti-Ztaq	[U-13C; U-15N]	protein	1.5 mM
2	Potassium Phosphate		buffer	20 mM
3	Sodium Chloride		salt	50 mM
4	Sodium Azide			0.01 %
5	D2O			10 % v/v

6 HN(CA)CO

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	anti-Ztaq	[U-13C; U-15N]	protein	1.5 mM
2	Potassium Phosphate		buffer	20 mM
3	Sodium Chloride		salt	50 mM
4	Sodium Azide			0.01 %
5	D2O			10 % v/v

7 HCCH-COSY

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	anti-Ztaq	[U-13C; U-15N]	protein	1.5 mM
2	Potassium Phosphate		buffer	20 mM
3	Sodium Chloride		salt	50 mM
4	Sodium Azide			0.01 %
5	D2O			10 % v/v

8 HCCH-TOCSY

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	anti-Ztaq	[U-13C; U-15N]	protein	1.5 mM
2	Potassium Phosphate		buffer	20 mM
3	Sodium Chloride		salt	50 mM
4	Sodium Azide			0.01 %
5	D2O			10 % v/v

9 (H)CC(CO)NH

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	anti-Ztaq	[U-13C; U-15N]	protein	1.5 mM
2	Potassium Phosphate		buffer	20 mM
3	Sodium Chloride		salt	50 mM
4	Sodium Azide			0.01 %
5	D2O			10 % v/v

10 15N-NOESY HSQC

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	anti-Ztaq	[U-13C; U-15N]	protein	1.5 mM
2	Potassium Phosphate		buffer	20 mM
3	Sodium Chloride		salt	50 mM
4	Sodium Azide			0.01 %
5	D2O			10 % v/v

11 15N-TOCSY HSQC

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	anti-Ztaq	[U-13C; U-15N]	protein	1.5 mM
2	Potassium Phosphate		buffer	20 mM
3	Sodium Chloride		salt	50 mM
4	Sodium Azide			0.01 %
5	D2O			10 % v/v

12 13C-NOESY HSQC

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	anti-Ztaq	[U-13C; U-15N]	protein	1.5 mM
2	Potassium Phosphate		buffer	20 mM
3	Sodium Chloride		salt	50 mM
4	Sodium Azide			0.01 %
5	D2O			10 % v/v

nullKeywords affibody