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Found 1 Document (2.179 seconds)

BMRB: 6806

2B87

Letter to the Editor: NMR assignments of the free and bound-state components of an anti-idiotypic affibody complex

Authors

Dogan, J., Lendel, C., Hard, T.

Assembly

Ztaq:anti-Ztaq

Entity

1. Ztaq (polymer, Thiol state: not present), 58 monomers, 6484.197 Da Detail

VDNKFNKELG WATWEIFNLP NLNGVQVKAF IDSLRDDPSQ SANLLAEAKK LNDAQAPK

2. anti-Ztaq (polymer, Thiol state: not present), 58 monomers, 6419.281 Da Detail

VDNKFNKERV IAIGEIMRLP NLNSLQVVAF INSLRDDPSQ SANLLAEAKK LNDAQAPK

Total weight

12903.478 Da

Max. entity weight

6484.197 Da

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 94.8 %, Completeness (bb): 97.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.8 % (1304 of 1376)	96.8 % (699 of 722)	91.2 % (476 of 522)	97.7 % (129 of 132)
Backbone	97.5 % (667 of 684)	97.4 % (223 of 229)	97.7 % (337 of 345)	97.3 % (107 of 110)
Sidechain	92.9 % (748 of 805)	96.6 % (476 of 493)	86.2 % (250 of 290)	100.0 % (22 of 22)
Aromatic	44.6 % (33 of 74)	83.8 % (31 of 37)	0.0 % (0 of 35)	100.0 % (2 of 2)
Methyl	98.6 % (136 of 138)	97.1 % (67 of 69)	100.0 % (69 of 69)

1. Ztaq

VDNKFNKELG WATWEIFNLP NLNGVQVKAF IDSLRDDPSQ SANLLAEAKK LNDAQAPK

2. anti-Ztaq

VDNKFNKERV IAIGEIMRLP NLNSLQVVAF INSLRDDPSQ SANLLAEAKK LNDAQAPK

Show sample condition

Sample #1

Pressure 1 (±0) atm, Temperature 298 (±0.1) K, Details Labelled anti-Ztaq in complex with unlabelled Ztaq

#	Name	Isotope labeling	Type	Concentration
1	Ztaq		protein	2.0 mM
2	anti-Ztaq	[U-13C; U-15N]	protein	1.5 mM
3	Potassium Phosphate		buffer	20 mM
4	Sodium Chloride		salt	50 mM
5	Sodium Azide			0.01 %
6	D2O			10 % v/v

Sample #2

Pressure 1 (±0) atm, Temperature 298 (±0.1) K, Details Labelled Ztaq in complex with unlabelled anti-Ztaq

#	Name	Isotope labeling	Type	Concentration
7	Ztaq	[U-13C; U-15N]	protein	1.3 mM
8	anti-Ztaq		protein	1.6 mM
9	Potassium Phosphate		buffer	20 mM
10	Sodium Chloride		salt	50 mM
11	Sodium Azide			0.01 %
12	D2O			10 % v/v

Protein Blocks Logo

Calculated from 40 models in PDB: 2B87, Strand ID: A, B Detail

Release date

2006-03-09

Citation

NMR assignments of the free and bound-state protein components of an anti-idiotypic affibody complex
Dogan, J., Lendel, C., Hard, T.
J. Biomol. NMR (2006), 36 Suppl 1, 13-13, PubMed 16456703 , DOI 10.1007/s10858-005-5350-8 , Abstract

Related entities 1. Ztaq, : 2 : 1 : 2 : 156 entities Detail

Related entities 2. anti-Ztaq, : 2 : 1 : 157 entities Detail

Experiments performed 12 experiments Detail

1 1H-15N HSQC

Instrument

#1 Model Bruker AVANCE (600 MHz)

#2 Model Bruker AVANCE (500 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Pressure 1 (±0) atm, Temperature 298 (±0.1) K, Details Labelled anti-Ztaq in complex with unlabelled Ztaq

#	Name	Isotope labeling	Type	Concentration
1	Ztaq		protein	2.0 mM
2	anti-Ztaq	[U-13C; U-15N]	protein	1.5 mM
3	Potassium Phosphate		buffer	20 mM
4	Sodium Chloride		salt	50 mM
5	Sodium Azide			0.01 %
6	D2O			10 % v/v

Sample #2

Pressure 1 (±0) atm, Temperature 298 (±0.1) K, Details Labelled Ztaq in complex with unlabelled anti-Ztaq

#	Name	Isotope labeling	Type	Concentration
7	Ztaq	[U-13C; U-15N]	protein	1.3 mM
8	anti-Ztaq		protein	1.6 mM
9	Potassium Phosphate		buffer	20 mM
10	Sodium Chloride		salt	50 mM
11	Sodium Azide			0.01 %
12	D2O			10 % v/v

2 1H-13C HSQC

Instrument

#1 Model Bruker AVANCE (600 MHz)

#2 Model Bruker AVANCE (500 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Pressure 1 (±0) atm, Temperature 298 (±0.1) K, Details Labelled anti-Ztaq in complex with unlabelled Ztaq

#	Name	Isotope labeling	Type	Concentration
1	Ztaq		protein	2.0 mM
2	anti-Ztaq	[U-13C; U-15N]	protein	1.5 mM
3	Potassium Phosphate		buffer	20 mM
4	Sodium Chloride		salt	50 mM
5	Sodium Azide			0.01 %
6	D2O			10 % v/v

Sample #2

Pressure 1 (±0) atm, Temperature 298 (±0.1) K, Details Labelled Ztaq in complex with unlabelled anti-Ztaq

#	Name	Isotope labeling	Type	Concentration
7	Ztaq	[U-13C; U-15N]	protein	1.3 mM
8	anti-Ztaq		protein	1.6 mM
9	Potassium Phosphate		buffer	20 mM
10	Sodium Chloride		salt	50 mM
11	Sodium Azide			0.01 %
12	D2O			10 % v/v

3 CBCA(CO)NH

Instrument

#1 Model Bruker AVANCE (600 MHz)

#2 Model Bruker AVANCE (500 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Pressure 1 (±0) atm, Temperature 298 (±0.1) K, Details Labelled anti-Ztaq in complex with unlabelled Ztaq

#	Name	Isotope labeling	Type	Concentration
1	Ztaq		protein	2.0 mM
2	anti-Ztaq	[U-13C; U-15N]	protein	1.5 mM
3	Potassium Phosphate		buffer	20 mM
4	Sodium Chloride		salt	50 mM
5	Sodium Azide			0.01 %
6	D2O			10 % v/v

Sample #2

Pressure 1 (±0) atm, Temperature 298 (±0.1) K, Details Labelled Ztaq in complex with unlabelled anti-Ztaq

#	Name	Isotope labeling	Type	Concentration
7	Ztaq	[U-13C; U-15N]	protein	1.3 mM
8	anti-Ztaq		protein	1.6 mM
9	Potassium Phosphate		buffer	20 mM
10	Sodium Chloride		salt	50 mM
11	Sodium Azide			0.01 %
12	D2O			10 % v/v

4 CBCANH

Instrument

#1 Model Bruker AVANCE (600 MHz)

#2 Model Bruker AVANCE (500 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Pressure 1 (±0) atm, Temperature 298 (±0.1) K, Details Labelled anti-Ztaq in complex with unlabelled Ztaq

#	Name	Isotope labeling	Type	Concentration
1	Ztaq		protein	2.0 mM
2	anti-Ztaq	[U-13C; U-15N]	protein	1.5 mM
3	Potassium Phosphate		buffer	20 mM
4	Sodium Chloride		salt	50 mM
5	Sodium Azide			0.01 %
6	D2O			10 % v/v

Sample #2

Pressure 1 (±0) atm, Temperature 298 (±0.1) K, Details Labelled Ztaq in complex with unlabelled anti-Ztaq

#	Name	Isotope labeling	Type	Concentration
7	Ztaq	[U-13C; U-15N]	protein	1.3 mM
8	anti-Ztaq		protein	1.6 mM
9	Potassium Phosphate		buffer	20 mM
10	Sodium Chloride		salt	50 mM
11	Sodium Azide			0.01 %
12	D2O			10 % v/v

5 HNCO

Instrument

#1 Model Bruker AVANCE (600 MHz)

#2 Model Bruker AVANCE (500 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Pressure 1 (±0) atm, Temperature 298 (±0.1) K, Details Labelled anti-Ztaq in complex with unlabelled Ztaq

#	Name	Isotope labeling	Type	Concentration
1	Ztaq		protein	2.0 mM
2	anti-Ztaq	[U-13C; U-15N]	protein	1.5 mM
3	Potassium Phosphate		buffer	20 mM
4	Sodium Chloride		salt	50 mM
5	Sodium Azide			0.01 %
6	D2O			10 % v/v

Sample #2

Pressure 1 (±0) atm, Temperature 298 (±0.1) K, Details Labelled Ztaq in complex with unlabelled anti-Ztaq

#	Name	Isotope labeling	Type	Concentration
7	Ztaq	[U-13C; U-15N]	protein	1.3 mM
8	anti-Ztaq		protein	1.6 mM
9	Potassium Phosphate		buffer	20 mM
10	Sodium Chloride		salt	50 mM
11	Sodium Azide			0.01 %
12	D2O			10 % v/v

6 HN(CA)CO

Instrument

#1 Model Bruker AVANCE (600 MHz)

#2 Model Bruker AVANCE (500 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Pressure 1 (±0) atm, Temperature 298 (±0.1) K, Details Labelled anti-Ztaq in complex with unlabelled Ztaq

#	Name	Isotope labeling	Type	Concentration
1	Ztaq		protein	2.0 mM
2	anti-Ztaq	[U-13C; U-15N]	protein	1.5 mM
3	Potassium Phosphate		buffer	20 mM
4	Sodium Chloride		salt	50 mM
5	Sodium Azide			0.01 %
6	D2O			10 % v/v

Sample #2

Pressure 1 (±0) atm, Temperature 298 (±0.1) K, Details Labelled Ztaq in complex with unlabelled anti-Ztaq

#	Name	Isotope labeling	Type	Concentration
7	Ztaq	[U-13C; U-15N]	protein	1.3 mM
8	anti-Ztaq		protein	1.6 mM
9	Potassium Phosphate		buffer	20 mM
10	Sodium Chloride		salt	50 mM
11	Sodium Azide			0.01 %
12	D2O			10 % v/v

7 HCCH-COSY

Instrument

#1 Model Bruker AVANCE (600 MHz)

#2 Model Bruker AVANCE (500 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Pressure 1 (±0) atm, Temperature 298 (±0.1) K, Details Labelled anti-Ztaq in complex with unlabelled Ztaq

#	Name	Isotope labeling	Type	Concentration
1	Ztaq		protein	2.0 mM
2	anti-Ztaq	[U-13C; U-15N]	protein	1.5 mM
3	Potassium Phosphate		buffer	20 mM
4	Sodium Chloride		salt	50 mM
5	Sodium Azide			0.01 %
6	D2O			10 % v/v

Sample #2

Pressure 1 (±0) atm, Temperature 298 (±0.1) K, Details Labelled Ztaq in complex with unlabelled anti-Ztaq

#	Name	Isotope labeling	Type	Concentration
7	Ztaq	[U-13C; U-15N]	protein	1.3 mM
8	anti-Ztaq		protein	1.6 mM
9	Potassium Phosphate		buffer	20 mM
10	Sodium Chloride		salt	50 mM
11	Sodium Azide			0.01 %
12	D2O			10 % v/v

8 HCCH-TOCSY

Instrument

#1 Model Bruker AVANCE (600 MHz)

#2 Model Bruker AVANCE (500 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Pressure 1 (±0) atm, Temperature 298 (±0.1) K, Details Labelled anti-Ztaq in complex with unlabelled Ztaq

#	Name	Isotope labeling	Type	Concentration
1	Ztaq		protein	2.0 mM
2	anti-Ztaq	[U-13C; U-15N]	protein	1.5 mM
3	Potassium Phosphate		buffer	20 mM
4	Sodium Chloride		salt	50 mM
5	Sodium Azide			0.01 %
6	D2O			10 % v/v

Sample #2

Pressure 1 (±0) atm, Temperature 298 (±0.1) K, Details Labelled Ztaq in complex with unlabelled anti-Ztaq

#	Name	Isotope labeling	Type	Concentration
7	Ztaq	[U-13C; U-15N]	protein	1.3 mM
8	anti-Ztaq		protein	1.6 mM
9	Potassium Phosphate		buffer	20 mM
10	Sodium Chloride		salt	50 mM
11	Sodium Azide			0.01 %
12	D2O			10 % v/v

9 (H)CC(CO)NH

Instrument

#1 Model Bruker AVANCE (600 MHz)

#2 Model Bruker AVANCE (500 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Pressure 1 (±0) atm, Temperature 298 (±0.1) K, Details Labelled anti-Ztaq in complex with unlabelled Ztaq

#	Name	Isotope labeling	Type	Concentration
1	Ztaq		protein	2.0 mM
2	anti-Ztaq	[U-13C; U-15N]	protein	1.5 mM
3	Potassium Phosphate		buffer	20 mM
4	Sodium Chloride		salt	50 mM
5	Sodium Azide			0.01 %
6	D2O			10 % v/v

Sample #2

Pressure 1 (±0) atm, Temperature 298 (±0.1) K, Details Labelled Ztaq in complex with unlabelled anti-Ztaq

#	Name	Isotope labeling	Type	Concentration
7	Ztaq	[U-13C; U-15N]	protein	1.3 mM
8	anti-Ztaq		protein	1.6 mM
9	Potassium Phosphate		buffer	20 mM
10	Sodium Chloride		salt	50 mM
11	Sodium Azide			0.01 %
12	D2O			10 % v/v

10 15N-NOESY HSQC

Instrument

#1 Model Bruker AVANCE (600 MHz)

#2 Model Bruker AVANCE (500 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Pressure 1 (±0) atm, Temperature 298 (±0.1) K, Details Labelled anti-Ztaq in complex with unlabelled Ztaq

#	Name	Isotope labeling	Type	Concentration
1	Ztaq		protein	2.0 mM
2	anti-Ztaq	[U-13C; U-15N]	protein	1.5 mM
3	Potassium Phosphate		buffer	20 mM
4	Sodium Chloride		salt	50 mM
5	Sodium Azide			0.01 %
6	D2O			10 % v/v

Sample #2

Pressure 1 (±0) atm, Temperature 298 (±0.1) K, Details Labelled Ztaq in complex with unlabelled anti-Ztaq

#	Name	Isotope labeling	Type	Concentration
7	Ztaq	[U-13C; U-15N]	protein	1.3 mM
8	anti-Ztaq		protein	1.6 mM
9	Potassium Phosphate		buffer	20 mM
10	Sodium Chloride		salt	50 mM
11	Sodium Azide			0.01 %
12	D2O			10 % v/v

11 15N-TOCSY HSQC

Instrument

#1 Model Bruker AVANCE (600 MHz)

#2 Model Bruker AVANCE (500 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Pressure 1 (±0) atm, Temperature 298 (±0.1) K, Details Labelled anti-Ztaq in complex with unlabelled Ztaq

#	Name	Isotope labeling	Type	Concentration
1	Ztaq		protein	2.0 mM
2	anti-Ztaq	[U-13C; U-15N]	protein	1.5 mM
3	Potassium Phosphate		buffer	20 mM
4	Sodium Chloride		salt	50 mM
5	Sodium Azide			0.01 %
6	D2O			10 % v/v

Sample #2

Pressure 1 (±0) atm, Temperature 298 (±0.1) K, Details Labelled Ztaq in complex with unlabelled anti-Ztaq

#	Name	Isotope labeling	Type	Concentration
7	Ztaq	[U-13C; U-15N]	protein	1.3 mM
8	anti-Ztaq		protein	1.6 mM
9	Potassium Phosphate		buffer	20 mM
10	Sodium Chloride		salt	50 mM
11	Sodium Azide			0.01 %
12	D2O			10 % v/v

12 13C-NOESY HSQC

Instrument

#1 Model Bruker AVANCE (600 MHz)

#2 Model Bruker AVANCE (500 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Pressure 1 (±0) atm, Temperature 298 (±0.1) K, Details Labelled anti-Ztaq in complex with unlabelled Ztaq

#	Name	Isotope labeling	Type	Concentration
1	Ztaq		protein	2.0 mM
2	anti-Ztaq	[U-13C; U-15N]	protein	1.5 mM
3	Potassium Phosphate		buffer	20 mM
4	Sodium Chloride		salt	50 mM
5	Sodium Azide			0.01 %
6	D2O			10 % v/v

Sample #2

Pressure 1 (±0) atm, Temperature 298 (±0.1) K, Details Labelled Ztaq in complex with unlabelled anti-Ztaq

#	Name	Isotope labeling	Type	Concentration
7	Ztaq	[U-13C; U-15N]	protein	1.3 mM
8	anti-Ztaq		protein	1.6 mM
9	Potassium Phosphate		buffer	20 mM
10	Sodium Chloride		salt	50 mM
11	Sodium Azide			0.01 %
12	D2O			10 % v/v

nullKeywords anti-idiotypic affibody complex

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Found 1 Document (2.179 seconds)

BMRB: 6806

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Related entities 1. Ztaq, : 2 : 1 : 2 : 156 entities Detail

Related entities 2. anti-Ztaq, : 2 : 1 : 157 entities Detail

Experiments performed 12 experiments Detail

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