BMRB: 6809

Backbone 1H,13C, and 15N chemical shift assignments for rabbitpox encoded viral chemokine inhibitor (vCCI)

Authors

DeRider, M.L., Zhang, L., LiWang, P.J.

Assembly

rabbitpox vCCI

Entity

1. rabbitpox vCCI (polymer, Thiol state: all disulfide bound), 242 monomers, 26128.57 Da Detail

MPASLQQSSS SSSSCTEEEN KHHMGIDVII KVTKQDQTPT NDKICQSVTE ITESESDPDP EVESEDDSTS VEDVDPPTTY YSIIGGGLRM NFGFTKCPQI KSISESADGN TVNARLSSVS PGQGKDSPAI THEEALAMIK DCEVSIDIRC SEEEKDSDIK THPVLGSNIS HKKVSYEDII GSTIVDTKCV KNLEFSVRIG DMCKESSELE VKDGFKYVDG SASKGATDDT SLIDSTKLKA CV

Formula weight

26128.57 Da

Entity Connection

disulfide 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS15:SG	1:CYS203:SG
2	disulfide	sing	1:CYS45:SG	1:CYS241:SG
3	disulfide	sing	1:CYS97:SG	1:CYS142:SG
4	disulfide	sing	1:CYS150:SG	1:CYS189:SG

Source organism

Rabbitpox virus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.9 %, Completeness: 44.3 %, Completeness (bb): 72.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	44.3 % (1169 of 2636)	24.1 % (329 of 1366)	62.0 % (635 of 1024)	83.3 % (205 of 246)
Backbone	72.6 % (1039 of 1432)	47.7 % (233 of 488)	84.8 % (604 of 712)	87.1 % (202 of 232)
Sidechain	23.3 % (334 of 1432)	10.9 % (96 of 878)	43.5 % (235 of 540)	21.4 % (3 of 14)
Aromatic	0.0 % (0 of 92)	0.0 % (0 of 46)	0.0 % (0 of 46)
Methyl	23.1 % (55 of 238)	19.3 % (23 of 119)	26.9 % (32 of 119)

1. vCCI

MPASLQQSSS SSSSCTEEEN KHHMGIDVII KVTKQDQTPT NDKICQSVTE ITESESDPDP EVESEDDSTS VEDVDPPTTY YSIIGGGLRM NFGFTKCPQI KSISESADGN TVNARLSSVS PGQGKDSPAI THEEALAMIK DCEVSIDIRC SEEEKDSDIK THPVLGSNIS HKKVSYEDII GSTIVDTKCV KNLEFSVRIG DMCKESSELE VKDGFKYVDG SASKGATDDT SLIDSTKLKA CV

Show sample condition

Sample

Temperature 310 (±0.1) K, pH 7.0 (±0.1), Details unbound vCCI

#	Name	Isotope labeling	Type	Concentration
1	vCCI	[U-2H; U-15N; U-13C]	protein	2 mM
2	NaCl		salt	100 (±10.0) mM
3	Phosphate Buffer		buffer	20 mM

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LACS Plot; CA

Referencing offset: 0.59 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.59 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.65 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 15 models in PDB: 2FIN, Strand ID: A Detail

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Release date

2006-04-26

Citation 1

Resonance assignments and secondary structure of vCCI, a 26 kDa CC chemokine inhibitor from rabbitpox virus
DeRider, M.L., Zhang, L., LiWang, P.J.
J. Biomol. NMR (2006), 36 Suppl 1, 22-22, PubMed 16534540 , DOI 10.1007/s10858-005-6276-x , Abstract

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Citation 2

The T1/35kDa family of poxvirus-secreted proteins bind chemokines and modulate leukocyte influx into virus-infected tissues
Graham, K.A., Lalani, A.S., Macen, J.L., Ness, T.L., Barry, M., Liu, L.Y., Lucas, A., Clark-Lewis, I., Moyer, R.W., Mcfadden, G.
Virology (1997), 229, 12-24, PubMed 9123853 , DOI 10.1006/viro.1996.8423 , Abstract

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Related entities 1. rabbitpox vCCI, : 1 : 1 : 1 : 9 entities Detail

Experiments performed 6 experiments Detail