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BMRB: 6810

2AYX

Letter to the Editor: Assignment of 1H, 13C and 15N resonances of the last 250 residues in Escherichia coli RcsC, including the phosphoreceiver domain.

Authors

Rogov, V., Loehr, F., Rogova, N., Klammt, C., Koglin, A., Bernhard, F., Doetsch, V.

Assembly

C'-RcsC

Entity

1. C'-RcsC (polymer, Thiol state: all free), 254 monomers, 27907.62 Da Detail

MGGSGVEGLS GKRCWLAVRN ASLCQFLETS LQRSGIVVTT YEGQEPTPED VLITDEVVSK KWQGRAVVTF CRRHIGIPLE KAPGEWVHSV AAPHELPALL ARIYLIEMES DDPANALPST DKAVSDNDDM MILVVDDHPI NRRLLADQLG SLGYQCKTAN DGVDALNVLS KNHIDIVLSD VNMPNMDGYR LTQRIRQLGL TLPVIGVTAN ALAEEKQRCL ESGMDSCLSK PVTLDVIKQT LTLYAERVRK SRDS

Formula weight

27907.62 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

Energy minimization

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.2 %, Completeness: 94.7 %, Completeness (bb): 96.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.7 % (2715 of 2866)	94.2 % (1398 of 1484)	96.2 % (1075 of 1117)	91.3 % (242 of 265)
Backbone	96.7 % (1450 of 1500)	95.3 % (489 of 513)	98.0 % (730 of 745)	95.5 % (231 of 242)
Sidechain	93.6 % (1500 of 1603)	93.7 % (910 of 971)	95.1 % (579 of 609)	47.8 % (11 of 23)
Aromatic	87.1 % (101 of 116)	87.9 % (51 of 58)	85.5 % (47 of 55)	100.0 % (3 of 3)
Methyl	97.9 % (321 of 328)	98.8 % (162 of 164)	97.0 % (159 of 164)

1. C'-RcsC

Show sample condition

Sample #1

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details U-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
1	C'-RcsC	[U-95% 15N]	protein	1 (±0.1) mM
2	Tris-HCl		buffer	33 (±1.0) mM
3	NaCl		salt	125 (±5.0) mM
4	TCEP		reducing agent	1 mM

Sample #2

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details U-13C, U-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
5	C'-RcsC	[U-95% 13C; U-95% 15N]	protein	1 (±0.1) mM
6	Tris-HCl		buffer	33 (±1.0) mM
7	NaCl		salt	125 (±5.0) mM
8	TCEP		reducing agent	1 mM

Sample #3

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Ala-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
9	C'-RcsC	[95% 15N]-ALA	protein	0.25 (±0.05) mM
10	Tris-HCl		buffer	33 (±1.0) mM
11	NaCl		salt	125 (±5.0) mM
12	TCEP		reducing agent	1 mM

Sample #4

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Asn-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
13	C'-RcsC	[95% 15N]-ASN	protein	0.25 (±0.05) mM
14	Tris-HCl		buffer	33 (±1.0) mM
15	NaCl		salt	125 (±5.0) mM
16	TCEP		reducing agent	1 mM

Sample #5

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Asp-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
17	C'-RcsC	[95% 15N]-ASP	protein	0.25 (±0.05) mM
18	Tris-HCl		buffer	33 (±1.0) mM
19	NaCl		salt	125 (±5.0) mM
20	TCEP		reducing agent	1 mM

Sample #6

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Arg-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
21	C'-RcsC	[95% 15N]-ARG	protein	0.25 (±0.05) mM
22	Tris-HCl		buffer	33 (±1.0) mM
23	NaCl		salt	125 (±5.0) mM
24	TCEP		reducing agent	1 mM

Sample #7

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Cys-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
25	C'-RcsC	[95% 15N]-CYS	protein	0.25 (±0.05) mM
26	Tris-HCl		buffer	33 (±1.0) mM
27	NaCl		salt	125 (±5.0) mM
28	TCEP		reducing agent	1 mM

Sample #8

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Gly-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
29	C'-RcsC	[95% 15N]-GLY	protein	0.25 (±0.05) mM
30	Tris-HCl		buffer	33 (±1.0) mM
31	NaCl		salt	125 (±5.0) mM
32	TCEP		reducing agent	1 mM

Sample #9

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Ser-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
33	C'-RcsC	[95% 15N]-SER	protein	0.25 (±0.05) mM
34	Tris-HCl		buffer	33 (±1.0) mM
35	NaCl		salt	125 (±5.0) mM
36	TCEP		reducing agent	1 mM

Sample #10

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Thr-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
37	C'-RcsC	[95% 15N]-THR	protein	0.25 (±0.05) mM
38	Tris-HCl		buffer	33 (±1.0) mM
39	NaCl		salt	125 (±5.0) mM
40	TCEP		reducing agent	1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.12 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.12 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.21 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2AYX, Strand ID: A Detail

Release date

2006-10-18

Citation

NMR assignment of 1H, 13C and 15N resonances of the truncated Escherichia coli RcsC (700-949), including the phosphoreceiver domain
Rogov, V.V., Loehr, F., Rogova, N., Klammt, C., Koglin, A., Bernhard, F., Doetsch, V.
J. Biomol. NMR (2007), 38, 165-165, PubMed 17036162 , DOI 10.1007/s10858-006-9059-0 , Abstract

Related entities 1. C'-RcsC, : 2 : 7 entities Detail

Interaction partners 1. C'-RcsC, : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 10 experiments Detail

1 1H15N_HSQC

Instrument

#1 Model Bruker Avance (700 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details U-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
1	C'-RcsC	[U-95% 15N]	protein	1 (±0.1) mM
2	Tris-HCl		buffer	33 (±1.0) mM
3	NaCl		salt	125 (±5.0) mM
4	TCEP		reducing agent	1 mM

Sample #2

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details U-13C, U-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
5	C'-RcsC	[U-95% 13C; U-95% 15N]	protein	1 (±0.1) mM
6	Tris-HCl		buffer	33 (±1.0) mM
7	NaCl		salt	125 (±5.0) mM
8	TCEP		reducing agent	1 mM

Sample #3

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Ala-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
9	C'-RcsC	[95% 15N]-ALA	protein	0.25 (±0.05) mM
10	Tris-HCl		buffer	33 (±1.0) mM
11	NaCl		salt	125 (±5.0) mM
12	TCEP		reducing agent	1 mM

Sample #4

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Asn-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
13	C'-RcsC	[95% 15N]-ASN	protein	0.25 (±0.05) mM
14	Tris-HCl		buffer	33 (±1.0) mM
15	NaCl		salt	125 (±5.0) mM
16	TCEP		reducing agent	1 mM

Sample #5

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Asp-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
17	C'-RcsC	[95% 15N]-ASP	protein	0.25 (±0.05) mM
18	Tris-HCl		buffer	33 (±1.0) mM
19	NaCl		salt	125 (±5.0) mM
20	TCEP		reducing agent	1 mM

Sample #6

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Arg-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
21	C'-RcsC	[95% 15N]-ARG	protein	0.25 (±0.05) mM
22	Tris-HCl		buffer	33 (±1.0) mM
23	NaCl		salt	125 (±5.0) mM
24	TCEP		reducing agent	1 mM

Sample #7

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Cys-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
25	C'-RcsC	[95% 15N]-CYS	protein	0.25 (±0.05) mM
26	Tris-HCl		buffer	33 (±1.0) mM
27	NaCl		salt	125 (±5.0) mM
28	TCEP		reducing agent	1 mM

Sample #8

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Gly-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
29	C'-RcsC	[95% 15N]-GLY	protein	0.25 (±0.05) mM
30	Tris-HCl		buffer	33 (±1.0) mM
31	NaCl		salt	125 (±5.0) mM
32	TCEP		reducing agent	1 mM

Sample #9

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Ser-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
33	C'-RcsC	[95% 15N]-SER	protein	0.25 (±0.05) mM
34	Tris-HCl		buffer	33 (±1.0) mM
35	NaCl		salt	125 (±5.0) mM
36	TCEP		reducing agent	1 mM

Sample #10

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Thr-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
37	C'-RcsC	[95% 15N]-THR	protein	0.25 (±0.05) mM
38	Tris-HCl		buffer	33 (±1.0) mM
39	NaCl		salt	125 (±5.0) mM
40	TCEP		reducing agent	1 mM

2 1H15N_TOCSY

Instrument

#1 Model Bruker Avance (700 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details U-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
1	C'-RcsC	[U-95% 15N]	protein	1 (±0.1) mM
2	Tris-HCl		buffer	33 (±1.0) mM
3	NaCl		salt	125 (±5.0) mM
4	TCEP		reducing agent	1 mM

Sample #2

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details U-13C, U-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
5	C'-RcsC	[U-95% 13C; U-95% 15N]	protein	1 (±0.1) mM
6	Tris-HCl		buffer	33 (±1.0) mM
7	NaCl		salt	125 (±5.0) mM
8	TCEP		reducing agent	1 mM

Sample #3

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Ala-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
9	C'-RcsC	[95% 15N]-ALA	protein	0.25 (±0.05) mM
10	Tris-HCl		buffer	33 (±1.0) mM
11	NaCl		salt	125 (±5.0) mM
12	TCEP		reducing agent	1 mM

Sample #4

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Asn-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
13	C'-RcsC	[95% 15N]-ASN	protein	0.25 (±0.05) mM
14	Tris-HCl		buffer	33 (±1.0) mM
15	NaCl		salt	125 (±5.0) mM
16	TCEP		reducing agent	1 mM

Sample #5

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Asp-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
17	C'-RcsC	[95% 15N]-ASP	protein	0.25 (±0.05) mM
18	Tris-HCl		buffer	33 (±1.0) mM
19	NaCl		salt	125 (±5.0) mM
20	TCEP		reducing agent	1 mM

Sample #6

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Arg-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
21	C'-RcsC	[95% 15N]-ARG	protein	0.25 (±0.05) mM
22	Tris-HCl		buffer	33 (±1.0) mM
23	NaCl		salt	125 (±5.0) mM
24	TCEP		reducing agent	1 mM

Sample #7

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Cys-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
25	C'-RcsC	[95% 15N]-CYS	protein	0.25 (±0.05) mM
26	Tris-HCl		buffer	33 (±1.0) mM
27	NaCl		salt	125 (±5.0) mM
28	TCEP		reducing agent	1 mM

Sample #8

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Gly-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
29	C'-RcsC	[95% 15N]-GLY	protein	0.25 (±0.05) mM
30	Tris-HCl		buffer	33 (±1.0) mM
31	NaCl		salt	125 (±5.0) mM
32	TCEP		reducing agent	1 mM

Sample #9

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Ser-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
33	C'-RcsC	[95% 15N]-SER	protein	0.25 (±0.05) mM
34	Tris-HCl		buffer	33 (±1.0) mM
35	NaCl		salt	125 (±5.0) mM
36	TCEP		reducing agent	1 mM

Sample #10

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Thr-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
37	C'-RcsC	[95% 15N]-THR	protein	0.25 (±0.05) mM
38	Tris-HCl		buffer	33 (±1.0) mM
39	NaCl		salt	125 (±5.0) mM
40	TCEP		reducing agent	1 mM

3 1H13C15N_(15N_1H)-TROSY-HNCO

Instrument

#1 Model Bruker Avance (700 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details U-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
1	C'-RcsC	[U-95% 15N]	protein	1 (±0.1) mM
2	Tris-HCl		buffer	33 (±1.0) mM
3	NaCl		salt	125 (±5.0) mM
4	TCEP		reducing agent	1 mM

Sample #2

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details U-13C, U-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
5	C'-RcsC	[U-95% 13C; U-95% 15N]	protein	1 (±0.1) mM
6	Tris-HCl		buffer	33 (±1.0) mM
7	NaCl		salt	125 (±5.0) mM
8	TCEP		reducing agent	1 mM

Sample #3

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Ala-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
9	C'-RcsC	[95% 15N]-ALA	protein	0.25 (±0.05) mM
10	Tris-HCl		buffer	33 (±1.0) mM
11	NaCl		salt	125 (±5.0) mM
12	TCEP		reducing agent	1 mM

Sample #4

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Asn-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
13	C'-RcsC	[95% 15N]-ASN	protein	0.25 (±0.05) mM
14	Tris-HCl		buffer	33 (±1.0) mM
15	NaCl		salt	125 (±5.0) mM
16	TCEP		reducing agent	1 mM

Sample #5

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Asp-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
17	C'-RcsC	[95% 15N]-ASP	protein	0.25 (±0.05) mM
18	Tris-HCl		buffer	33 (±1.0) mM
19	NaCl		salt	125 (±5.0) mM
20	TCEP		reducing agent	1 mM

Sample #6

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Arg-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
21	C'-RcsC	[95% 15N]-ARG	protein	0.25 (±0.05) mM
22	Tris-HCl		buffer	33 (±1.0) mM
23	NaCl		salt	125 (±5.0) mM
24	TCEP		reducing agent	1 mM

Sample #7

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Cys-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
25	C'-RcsC	[95% 15N]-CYS	protein	0.25 (±0.05) mM
26	Tris-HCl		buffer	33 (±1.0) mM
27	NaCl		salt	125 (±5.0) mM
28	TCEP		reducing agent	1 mM

Sample #8

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Gly-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
29	C'-RcsC	[95% 15N]-GLY	protein	0.25 (±0.05) mM
30	Tris-HCl		buffer	33 (±1.0) mM
31	NaCl		salt	125 (±5.0) mM
32	TCEP		reducing agent	1 mM

Sample #9

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Ser-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
33	C'-RcsC	[95% 15N]-SER	protein	0.25 (±0.05) mM
34	Tris-HCl		buffer	33 (±1.0) mM
35	NaCl		salt	125 (±5.0) mM
36	TCEP		reducing agent	1 mM

Sample #10

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Thr-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
37	C'-RcsC	[95% 15N]-THR	protein	0.25 (±0.05) mM
38	Tris-HCl		buffer	33 (±1.0) mM
39	NaCl		salt	125 (±5.0) mM
40	TCEP		reducing agent	1 mM

4 1H13C15N_(15N_1H)-TROSY-HNCACB

Instrument

#1 Model Bruker Avance (700 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details U-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
1	C'-RcsC	[U-95% 15N]	protein	1 (±0.1) mM
2	Tris-HCl		buffer	33 (±1.0) mM
3	NaCl		salt	125 (±5.0) mM
4	TCEP		reducing agent	1 mM

Sample #2

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details U-13C, U-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
5	C'-RcsC	[U-95% 13C; U-95% 15N]	protein	1 (±0.1) mM
6	Tris-HCl		buffer	33 (±1.0) mM
7	NaCl		salt	125 (±5.0) mM
8	TCEP		reducing agent	1 mM

Sample #3

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Ala-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
9	C'-RcsC	[95% 15N]-ALA	protein	0.25 (±0.05) mM
10	Tris-HCl		buffer	33 (±1.0) mM
11	NaCl		salt	125 (±5.0) mM
12	TCEP		reducing agent	1 mM

Sample #4

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Asn-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
13	C'-RcsC	[95% 15N]-ASN	protein	0.25 (±0.05) mM
14	Tris-HCl		buffer	33 (±1.0) mM
15	NaCl		salt	125 (±5.0) mM
16	TCEP		reducing agent	1 mM

Sample #5

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Asp-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
17	C'-RcsC	[95% 15N]-ASP	protein	0.25 (±0.05) mM
18	Tris-HCl		buffer	33 (±1.0) mM
19	NaCl		salt	125 (±5.0) mM
20	TCEP		reducing agent	1 mM

Sample #6

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Arg-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
21	C'-RcsC	[95% 15N]-ARG	protein	0.25 (±0.05) mM
22	Tris-HCl		buffer	33 (±1.0) mM
23	NaCl		salt	125 (±5.0) mM
24	TCEP		reducing agent	1 mM

Sample #7

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Cys-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
25	C'-RcsC	[95% 15N]-CYS	protein	0.25 (±0.05) mM
26	Tris-HCl		buffer	33 (±1.0) mM
27	NaCl		salt	125 (±5.0) mM
28	TCEP		reducing agent	1 mM

Sample #8

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Gly-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
29	C'-RcsC	[95% 15N]-GLY	protein	0.25 (±0.05) mM
30	Tris-HCl		buffer	33 (±1.0) mM
31	NaCl		salt	125 (±5.0) mM
32	TCEP		reducing agent	1 mM

Sample #9

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Ser-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
33	C'-RcsC	[95% 15N]-SER	protein	0.25 (±0.05) mM
34	Tris-HCl		buffer	33 (±1.0) mM
35	NaCl		salt	125 (±5.0) mM
36	TCEP		reducing agent	1 mM

Sample #10

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Thr-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
37	C'-RcsC	[95% 15N]-THR	protein	0.25 (±0.05) mM
38	Tris-HCl		buffer	33 (±1.0) mM
39	NaCl		salt	125 (±5.0) mM
40	TCEP		reducing agent	1 mM

5 1H13C15N_(HCA)CO(CA)NH

Instrument

#1 Model Bruker Avance (700 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details U-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
1	C'-RcsC	[U-95% 15N]	protein	1 (±0.1) mM
2	Tris-HCl		buffer	33 (±1.0) mM
3	NaCl		salt	125 (±5.0) mM
4	TCEP		reducing agent	1 mM

Sample #2

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details U-13C, U-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
5	C'-RcsC	[U-95% 13C; U-95% 15N]	protein	1 (±0.1) mM
6	Tris-HCl		buffer	33 (±1.0) mM
7	NaCl		salt	125 (±5.0) mM
8	TCEP		reducing agent	1 mM

Sample #3

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Ala-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
9	C'-RcsC	[95% 15N]-ALA	protein	0.25 (±0.05) mM
10	Tris-HCl		buffer	33 (±1.0) mM
11	NaCl		salt	125 (±5.0) mM
12	TCEP		reducing agent	1 mM

Sample #4

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Asn-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
13	C'-RcsC	[95% 15N]-ASN	protein	0.25 (±0.05) mM
14	Tris-HCl		buffer	33 (±1.0) mM
15	NaCl		salt	125 (±5.0) mM
16	TCEP		reducing agent	1 mM

Sample #5

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Asp-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
17	C'-RcsC	[95% 15N]-ASP	protein	0.25 (±0.05) mM
18	Tris-HCl		buffer	33 (±1.0) mM
19	NaCl		salt	125 (±5.0) mM
20	TCEP		reducing agent	1 mM

Sample #6

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Arg-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
21	C'-RcsC	[95% 15N]-ARG	protein	0.25 (±0.05) mM
22	Tris-HCl		buffer	33 (±1.0) mM
23	NaCl		salt	125 (±5.0) mM
24	TCEP		reducing agent	1 mM

Sample #7

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Cys-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
25	C'-RcsC	[95% 15N]-CYS	protein	0.25 (±0.05) mM
26	Tris-HCl		buffer	33 (±1.0) mM
27	NaCl		salt	125 (±5.0) mM
28	TCEP		reducing agent	1 mM

Sample #8

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Gly-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
29	C'-RcsC	[95% 15N]-GLY	protein	0.25 (±0.05) mM
30	Tris-HCl		buffer	33 (±1.0) mM
31	NaCl		salt	125 (±5.0) mM
32	TCEP		reducing agent	1 mM

Sample #9

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Ser-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
33	C'-RcsC	[95% 15N]-SER	protein	0.25 (±0.05) mM
34	Tris-HCl		buffer	33 (±1.0) mM
35	NaCl		salt	125 (±5.0) mM
36	TCEP		reducing agent	1 mM

Sample #10

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Thr-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
37	C'-RcsC	[95% 15N]-THR	protein	0.25 (±0.05) mM
38	Tris-HCl		buffer	33 (±1.0) mM
39	NaCl		salt	125 (±5.0) mM
40	TCEP		reducing agent	1 mM

6 1H13C15N_H(CC)(CO)NH-TOCSY

Instrument

#1 Model Bruker Avance (700 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details U-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
1	C'-RcsC	[U-95% 15N]	protein	1 (±0.1) mM
2	Tris-HCl		buffer	33 (±1.0) mM
3	NaCl		salt	125 (±5.0) mM
4	TCEP		reducing agent	1 mM

Sample #2

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details U-13C, U-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
5	C'-RcsC	[U-95% 13C; U-95% 15N]	protein	1 (±0.1) mM
6	Tris-HCl		buffer	33 (±1.0) mM
7	NaCl		salt	125 (±5.0) mM
8	TCEP		reducing agent	1 mM

Sample #3

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Ala-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
9	C'-RcsC	[95% 15N]-ALA	protein	0.25 (±0.05) mM
10	Tris-HCl		buffer	33 (±1.0) mM
11	NaCl		salt	125 (±5.0) mM
12	TCEP		reducing agent	1 mM

Sample #4

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Asn-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
13	C'-RcsC	[95% 15N]-ASN	protein	0.25 (±0.05) mM
14	Tris-HCl		buffer	33 (±1.0) mM
15	NaCl		salt	125 (±5.0) mM
16	TCEP		reducing agent	1 mM

Sample #5

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Asp-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
17	C'-RcsC	[95% 15N]-ASP	protein	0.25 (±0.05) mM
18	Tris-HCl		buffer	33 (±1.0) mM
19	NaCl		salt	125 (±5.0) mM
20	TCEP		reducing agent	1 mM

Sample #6

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Arg-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
21	C'-RcsC	[95% 15N]-ARG	protein	0.25 (±0.05) mM
22	Tris-HCl		buffer	33 (±1.0) mM
23	NaCl		salt	125 (±5.0) mM
24	TCEP		reducing agent	1 mM

Sample #7

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Cys-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
25	C'-RcsC	[95% 15N]-CYS	protein	0.25 (±0.05) mM
26	Tris-HCl		buffer	33 (±1.0) mM
27	NaCl		salt	125 (±5.0) mM
28	TCEP		reducing agent	1 mM

Sample #8

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Gly-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
29	C'-RcsC	[95% 15N]-GLY	protein	0.25 (±0.05) mM
30	Tris-HCl		buffer	33 (±1.0) mM
31	NaCl		salt	125 (±5.0) mM
32	TCEP		reducing agent	1 mM

Sample #9

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Ser-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
33	C'-RcsC	[95% 15N]-SER	protein	0.25 (±0.05) mM
34	Tris-HCl		buffer	33 (±1.0) mM
35	NaCl		salt	125 (±5.0) mM
36	TCEP		reducing agent	1 mM

Sample #10

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Thr-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
37	C'-RcsC	[95% 15N]-THR	protein	0.25 (±0.05) mM
38	Tris-HCl		buffer	33 (±1.0) mM
39	NaCl		salt	125 (±5.0) mM
40	TCEP		reducing agent	1 mM

7 1H13C15N_(H)CC(CO)NH-TOCSY

Instrument

#1 Model Bruker Avance (700 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details U-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
1	C'-RcsC	[U-95% 15N]	protein	1 (±0.1) mM
2	Tris-HCl		buffer	33 (±1.0) mM
3	NaCl		salt	125 (±5.0) mM
4	TCEP		reducing agent	1 mM

Sample #2

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details U-13C, U-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
5	C'-RcsC	[U-95% 13C; U-95% 15N]	protein	1 (±0.1) mM
6	Tris-HCl		buffer	33 (±1.0) mM
7	NaCl		salt	125 (±5.0) mM
8	TCEP		reducing agent	1 mM

Sample #3

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Ala-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
9	C'-RcsC	[95% 15N]-ALA	protein	0.25 (±0.05) mM
10	Tris-HCl		buffer	33 (±1.0) mM
11	NaCl		salt	125 (±5.0) mM
12	TCEP		reducing agent	1 mM

Sample #4

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Asn-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
13	C'-RcsC	[95% 15N]-ASN	protein	0.25 (±0.05) mM
14	Tris-HCl		buffer	33 (±1.0) mM
15	NaCl		salt	125 (±5.0) mM
16	TCEP		reducing agent	1 mM

Sample #5

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Asp-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
17	C'-RcsC	[95% 15N]-ASP	protein	0.25 (±0.05) mM
18	Tris-HCl		buffer	33 (±1.0) mM
19	NaCl		salt	125 (±5.0) mM
20	TCEP		reducing agent	1 mM

Sample #6

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Arg-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
21	C'-RcsC	[95% 15N]-ARG	protein	0.25 (±0.05) mM
22	Tris-HCl		buffer	33 (±1.0) mM
23	NaCl		salt	125 (±5.0) mM
24	TCEP		reducing agent	1 mM

Sample #7

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Cys-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
25	C'-RcsC	[95% 15N]-CYS	protein	0.25 (±0.05) mM
26	Tris-HCl		buffer	33 (±1.0) mM
27	NaCl		salt	125 (±5.0) mM
28	TCEP		reducing agent	1 mM

Sample #8

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Gly-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
29	C'-RcsC	[95% 15N]-GLY	protein	0.25 (±0.05) mM
30	Tris-HCl		buffer	33 (±1.0) mM
31	NaCl		salt	125 (±5.0) mM
32	TCEP		reducing agent	1 mM

Sample #9

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Ser-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
33	C'-RcsC	[95% 15N]-SER	protein	0.25 (±0.05) mM
34	Tris-HCl		buffer	33 (±1.0) mM
35	NaCl		salt	125 (±5.0) mM
36	TCEP		reducing agent	1 mM

Sample #10

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Thr-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
37	C'-RcsC	[95% 15N]-THR	protein	0.25 (±0.05) mM
38	Tris-HCl		buffer	33 (±1.0) mM
39	NaCl		salt	125 (±5.0) mM
40	TCEP		reducing agent	1 mM

8 1H13C15N_H(CDCGCBCACO)NH

Instrument

#1 Model Bruker Avance (700 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details U-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
1	C'-RcsC	[U-95% 15N]	protein	1 (±0.1) mM
2	Tris-HCl		buffer	33 (±1.0) mM
3	NaCl		salt	125 (±5.0) mM
4	TCEP		reducing agent	1 mM

Sample #2

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details U-13C, U-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
5	C'-RcsC	[U-95% 13C; U-95% 15N]	protein	1 (±0.1) mM
6	Tris-HCl		buffer	33 (±1.0) mM
7	NaCl		salt	125 (±5.0) mM
8	TCEP		reducing agent	1 mM

Sample #3

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Ala-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
9	C'-RcsC	[95% 15N]-ALA	protein	0.25 (±0.05) mM
10	Tris-HCl		buffer	33 (±1.0) mM
11	NaCl		salt	125 (±5.0) mM
12	TCEP		reducing agent	1 mM

Sample #4

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Asn-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
13	C'-RcsC	[95% 15N]-ASN	protein	0.25 (±0.05) mM
14	Tris-HCl		buffer	33 (±1.0) mM
15	NaCl		salt	125 (±5.0) mM
16	TCEP		reducing agent	1 mM

Sample #5

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Asp-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
17	C'-RcsC	[95% 15N]-ASP	protein	0.25 (±0.05) mM
18	Tris-HCl		buffer	33 (±1.0) mM
19	NaCl		salt	125 (±5.0) mM
20	TCEP		reducing agent	1 mM

Sample #6

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Arg-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
21	C'-RcsC	[95% 15N]-ARG	protein	0.25 (±0.05) mM
22	Tris-HCl		buffer	33 (±1.0) mM
23	NaCl		salt	125 (±5.0) mM
24	TCEP		reducing agent	1 mM

Sample #7

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Cys-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
25	C'-RcsC	[95% 15N]-CYS	protein	0.25 (±0.05) mM
26	Tris-HCl		buffer	33 (±1.0) mM
27	NaCl		salt	125 (±5.0) mM
28	TCEP		reducing agent	1 mM

Sample #8

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Gly-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
29	C'-RcsC	[95% 15N]-GLY	protein	0.25 (±0.05) mM
30	Tris-HCl		buffer	33 (±1.0) mM
31	NaCl		salt	125 (±5.0) mM
32	TCEP		reducing agent	1 mM

Sample #9

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Ser-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
33	C'-RcsC	[95% 15N]-SER	protein	0.25 (±0.05) mM
34	Tris-HCl		buffer	33 (±1.0) mM
35	NaCl		salt	125 (±5.0) mM
36	TCEP		reducing agent	1 mM

Sample #10

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Thr-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
37	C'-RcsC	[95% 15N]-THR	protein	0.25 (±0.05) mM
38	Tris-HCl		buffer	33 (±1.0) mM
39	NaCl		salt	125 (±5.0) mM
40	TCEP		reducing agent	1 mM

9 1H13C15N_(H)CD(CGCBCACO)NH

Instrument

#1 Model Bruker Avance (700 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details U-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
1	C'-RcsC	[U-95% 15N]	protein	1 (±0.1) mM
2	Tris-HCl		buffer	33 (±1.0) mM
3	NaCl		salt	125 (±5.0) mM
4	TCEP		reducing agent	1 mM

Sample #2

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details U-13C, U-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
5	C'-RcsC	[U-95% 13C; U-95% 15N]	protein	1 (±0.1) mM
6	Tris-HCl		buffer	33 (±1.0) mM
7	NaCl		salt	125 (±5.0) mM
8	TCEP		reducing agent	1 mM

Sample #3

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Ala-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
9	C'-RcsC	[95% 15N]-ALA	protein	0.25 (±0.05) mM
10	Tris-HCl		buffer	33 (±1.0) mM
11	NaCl		salt	125 (±5.0) mM
12	TCEP		reducing agent	1 mM

Sample #4

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Asn-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
13	C'-RcsC	[95% 15N]-ASN	protein	0.25 (±0.05) mM
14	Tris-HCl		buffer	33 (±1.0) mM
15	NaCl		salt	125 (±5.0) mM
16	TCEP		reducing agent	1 mM

Sample #5

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Asp-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
17	C'-RcsC	[95% 15N]-ASP	protein	0.25 (±0.05) mM
18	Tris-HCl		buffer	33 (±1.0) mM
19	NaCl		salt	125 (±5.0) mM
20	TCEP		reducing agent	1 mM

Sample #6

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Arg-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
21	C'-RcsC	[95% 15N]-ARG	protein	0.25 (±0.05) mM
22	Tris-HCl		buffer	33 (±1.0) mM
23	NaCl		salt	125 (±5.0) mM
24	TCEP		reducing agent	1 mM

Sample #7

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Cys-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
25	C'-RcsC	[95% 15N]-CYS	protein	0.25 (±0.05) mM
26	Tris-HCl		buffer	33 (±1.0) mM
27	NaCl		salt	125 (±5.0) mM
28	TCEP		reducing agent	1 mM

Sample #8

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Gly-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
29	C'-RcsC	[95% 15N]-GLY	protein	0.25 (±0.05) mM
30	Tris-HCl		buffer	33 (±1.0) mM
31	NaCl		salt	125 (±5.0) mM
32	TCEP		reducing agent	1 mM

Sample #9

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Ser-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
33	C'-RcsC	[95% 15N]-SER	protein	0.25 (±0.05) mM
34	Tris-HCl		buffer	33 (±1.0) mM
35	NaCl		salt	125 (±5.0) mM
36	TCEP		reducing agent	1 mM

Sample #10

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Thr-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
37	C'-RcsC	[95% 15N]-THR	protein	0.25 (±0.05) mM
38	Tris-HCl		buffer	33 (±1.0) mM
39	NaCl		salt	125 (±5.0) mM
40	TCEP		reducing agent	1 mM

10 1H13C15N_HC(CN)H-TOCSY_TRP

Instrument

#1 Model Bruker Avance (700 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details U-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
1	C'-RcsC	[U-95% 15N]	protein	1 (±0.1) mM
2	Tris-HCl		buffer	33 (±1.0) mM
3	NaCl		salt	125 (±5.0) mM
4	TCEP		reducing agent	1 mM

Sample #2

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details U-13C, U-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
5	C'-RcsC	[U-95% 13C; U-95% 15N]	protein	1 (±0.1) mM
6	Tris-HCl		buffer	33 (±1.0) mM
7	NaCl		salt	125 (±5.0) mM
8	TCEP		reducing agent	1 mM

Sample #3

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Ala-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
9	C'-RcsC	[95% 15N]-ALA	protein	0.25 (±0.05) mM
10	Tris-HCl		buffer	33 (±1.0) mM
11	NaCl		salt	125 (±5.0) mM
12	TCEP		reducing agent	1 mM

Sample #4

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Asn-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
13	C'-RcsC	[95% 15N]-ASN	protein	0.25 (±0.05) mM
14	Tris-HCl		buffer	33 (±1.0) mM
15	NaCl		salt	125 (±5.0) mM
16	TCEP		reducing agent	1 mM

Sample #5

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Asp-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
17	C'-RcsC	[95% 15N]-ASP	protein	0.25 (±0.05) mM
18	Tris-HCl		buffer	33 (±1.0) mM
19	NaCl		salt	125 (±5.0) mM
20	TCEP		reducing agent	1 mM

Sample #6

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Arg-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
21	C'-RcsC	[95% 15N]-ARG	protein	0.25 (±0.05) mM
22	Tris-HCl		buffer	33 (±1.0) mM
23	NaCl		salt	125 (±5.0) mM
24	TCEP		reducing agent	1 mM

Sample #7

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Cys-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
25	C'-RcsC	[95% 15N]-CYS	protein	0.25 (±0.05) mM
26	Tris-HCl		buffer	33 (±1.0) mM
27	NaCl		salt	125 (±5.0) mM
28	TCEP		reducing agent	1 mM

Sample #8

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Gly-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
29	C'-RcsC	[95% 15N]-GLY	protein	0.25 (±0.05) mM
30	Tris-HCl		buffer	33 (±1.0) mM
31	NaCl		salt	125 (±5.0) mM
32	TCEP		reducing agent	1 mM

Sample #9

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Ser-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
33	C'-RcsC	[95% 15N]-SER	protein	0.25 (±0.05) mM
34	Tris-HCl		buffer	33 (±1.0) mM
35	NaCl		salt	125 (±5.0) mM
36	TCEP		reducing agent	1 mM

Sample #10

Pressure 1 (±0.001) atm, Temperature 296 (±1) K, pH 7.5 (±0.1), Details Thr-15N labeled C'-RcsC

#	Name	Isotope labeling	Type	Concentration
37	C'-RcsC	[95% 15N]-THR	protein	0.25 (±0.05) mM
38	Tris-HCl		buffer	33 (±1.0) mM
39	NaCl		salt	125 (±5.0) mM
40	TCEP		reducing agent	1 mM

nullKeywords cell-free protein biosynthesis, sensor kinase

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BMRB: 6810

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Chemical shift reference

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Chemical shift reference

Related entities 1. C'-RcsC, : 2 : 7 entities Detail

Interaction partners 1. C'-RcsC, : 2 interactors Detail

Experiments performed 10 experiments Detail

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