BMRB: 6811

Solution structure of apoCadA

Authors

Banci, L., Bertini, I., Ciofi-Baffoni, S., Su, X., Miras, R., Bal, N., Mintz, E., Catty, P., Shokes, J.E., Scott, R.A.

Assembly

cadmium-transporting ATPase (E.C.3.6.3.3)

Entity

1. cadmium-transporting ATPase (E.C.3.6.3.3) (polymer, Thiol state: all free), 71 monomers, 7699.653 Da Detail

MAEKTVYRVD GLSCTNCAAK FERNVKEIEG VTEAIVNFGA SKITVTGEAS IQQVEQAGAF EHLKIIPEKE A

Formula weight

7699.653 Da

Source organism

Listeria monocytogenes

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.6 %, Completeness: 93.0 %, Completeness (bb): 97.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	93.0 % (454 of 488)	92.2 % (380 of 412)	97.4 % (74 of 76)
Backbone	97.7 % (211 of 216)	97.9 % (143 of 146)	97.1 % (68 of 70)
Sidechain	89.3 % (243 of 272)	89.1 % (237 of 266)	100.0 % (6 of 6)
Aromatic	90.5 % (19 of 21)	90.5 % (19 of 21)
Methyl	97.7 % (43 of 44)	97.7 % (43 of 44)

1. P-type ATPase

MAEKTVYRVD GLSCTNCAAK FERNVKEIEG VTEAIVNFGA SKITVTGEAS IQQVEQAGAF EHLKIIPEKE A

Show sample condition

Sample

Pressure 1 atm, Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	P-type ATPase	[U-15N]		2 mM
2	sodium phosphate buffer			350 mM
3	TCEP			5 mM
4	H2O			90 %
5	D2O			10 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2AJ0, Strand ID: A Detail

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Release date

2007-01-25

Citation

Structural basis for metal binding specificity: the N-terminal cadmium binding domain of the P1-type ATPase CadA
Banci, L., Bertini, I., Ciofi-Baffoni, S., Su, X., Miras, R., Bal, N., Mintz, E., Catty, P., Shokes, J.E., Scott, R.A.
J. Mol. Biol. (2006), 356, 638-650, PubMed 16388822 , DOI 10.1016/j.jmb.2005.11.055 , Abstract

Related entities 1. cadmium-transporting ATPase (E.C.3.6.3.3), : 1 : 1 : 1 : 50 entities Detail

Experiments performed 5 experiments Detail