BMRBj - BMREntryMaster

Found 1 Document (1.623 seconds)

BMRB: 6814

2AU4

Class I GTP aptamer

Authors

Carothers, J.M., Davis, J.H., Chou, J.J., Szostak, J.W.

Assembly

Class I RNA aptamer to GTP

Entity

1. Class I RNA aptamer to GTP (polymer, Thiol state: not present), 41 monomers, 13387.90 Da Detail

GGGACGAAGU GGUUGGGCGC UUCGGCGUGU GAAAACGUCU C

2. GTP (non-polymer), 523.180 Da

Total weight

13911.08 Da

Max. entity weight

13387.9 Da

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 90.2 %, Completeness: 75.1 %, Completeness (bb): 70.3 % Detail

Polymer type: polyribonucleotide

	Total	¹H
All	75.1 % (265 of 353)	75.1 % (265 of 353)
Suger, PO4	70.3 % (173 of 246)	70.3 % (173 of 246)
Nucleobase	86.0 % (92 of 107)	86.0 % (92 of 107)
Aromatic	85.7 % (78 of 91)	85.7 % (78 of 91)

1. Class I RNA aptamer to GTP

GGGACGAAGU GGUUGGGCGC UUCGGCGUGU GAAAACGUCU C

Show sample condition

Sample

Pressure 1 atm, Temperature 288 K, pH 6.1

#	Name	Isotope labeling	Concentration
1	Class I RNA aptamer to GTP	[U-15N; U-13C]	3.3 mM
2	GUANOSINE-5'-TRIPHOSPHATE		3.63 mM
3	phosphate buffer		10 %
4	D2O		99 %

Chem. Shift Complete2

Sequence coverage: 0.0 %, Completeness: 37.5 %, Completeness (bb): 35.2 % Detail

Polymer type: polyribonucleotide

	Total	¹H
All	37.5 % (265 of 706)	37.5 % (265 of 706)
Suger, PO4	35.2 % (173 of 492)	35.2 % (173 of 492)
Nucleobase	43.0 % (92 of 214)	43.0 % (92 of 214)
Aromatic	42.9 % (78 of 182)	42.9 % (78 of 182)

1. Class I RNA aptamer to GTP

GGGACGAAGU GGUUGGGCGC UUCGGCGUGU GAAAACGUCU C

Show sample condition

Sample

Pressure 1 atm, Temperature 288 K, pH 6.1

#	Name	Isotope labeling	Concentration
1	Class I RNA aptamer to GTP	[U-15N; U-13C]	3.3 mM
2	GUANOSINE-5'-TRIPHOSPHATE		3.63 mM
3	phosphate buffer		10 %
4	D2O		99 %

Release date

2007-07-15

Citation

Solution structure of an informationally complex high-affinity RNA aptamer to GTP
Carothers, J.M., Davis, J.H., Chou, J.J., Szostak, J.W.
RNA (2006), 12, 567-579, PubMed 16510427 , DOI 10.1261/rna.2251306 , Abstract

Related entities 1. Class I RNA aptamer to GTP, : 1 entities Detail

Experiments performed 6 experiments Detail

1 3D 13C-separated NOESY

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm

#	Name	Isotope labeling	Concentration
1	Class I RNA aptamer to GTP	[U-15N; U-13C]	3.3 mM
2	GUANOSINE-5'-TRIPHOSPHATE		3.63 mM
3	phosphate buffer		10 %
4	D2O		99 %

Sample #2

Pressure 1 atm

#	Name	Isotope labeling	Concentration
5	Class I RNA aptamer to GTP	[U-15N; U-13C]	0.9 mM
6	GUANOSINE-5'-TRIPHOSPHATE		0.99 mM
7	phosphate buffer		10 %
8	D2O		99 %

Sample #3

Pressure 1 atm

#	Name	Isotope labeling	Concentration
9	Class I RNA aptamer to GTP	[U-15N; U-13C]	3.3 mM
10	GUANOSINE-5'-TRIPHOSPHATE		3.63 mM
11	phosphate buffer		10 %
12	D2O		5 %
13	H2O		95 %

Sample #4

Pressure 1 atm

#	Name	Isotope labeling	Concentration
14	Class I RNA aptamer to GTP	[U-15N; U-13C]	0.9 mM
15	GUANOSINE-5'-TRIPHOSPHATE		0.99 mM
16	phosphate buffer		10 %
17	D2O		5 %
18	H2O		95 %

2 3D 15N-separated NOESY

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm

#	Name	Isotope labeling	Concentration
1	Class I RNA aptamer to GTP	[U-15N; U-13C]	3.3 mM
2	GUANOSINE-5'-TRIPHOSPHATE		3.63 mM
3	phosphate buffer		10 %
4	D2O		99 %

Sample #2

Pressure 1 atm

#	Name	Isotope labeling	Concentration
5	Class I RNA aptamer to GTP	[U-15N; U-13C]	0.9 mM
6	GUANOSINE-5'-TRIPHOSPHATE		0.99 mM
7	phosphate buffer		10 %
8	D2O		99 %

Sample #3

Pressure 1 atm

#	Name	Isotope labeling	Concentration
9	Class I RNA aptamer to GTP	[U-15N; U-13C]	3.3 mM
10	GUANOSINE-5'-TRIPHOSPHATE		3.63 mM
11	phosphate buffer		10 %
12	D2O		5 %
13	H2O		95 %

Sample #4

Pressure 1 atm

#	Name	Isotope labeling	Concentration
14	Class I RNA aptamer to GTP	[U-15N; U-13C]	0.9 mM
15	GUANOSINE-5'-TRIPHOSPHATE		0.99 mM
16	phosphate buffer		10 %
17	D2O		5 %
18	H2O		95 %

3 2D NOESY

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm

#	Name	Isotope labeling	Concentration
1	Class I RNA aptamer to GTP	[U-15N; U-13C]	3.3 mM
2	GUANOSINE-5'-TRIPHOSPHATE		3.63 mM
3	phosphate buffer		10 %
4	D2O		99 %

Sample #2

Pressure 1 atm

#	Name	Isotope labeling	Concentration
5	Class I RNA aptamer to GTP	[U-15N; U-13C]	0.9 mM
6	GUANOSINE-5'-TRIPHOSPHATE		0.99 mM
7	phosphate buffer		10 %
8	D2O		99 %

Sample #3

Pressure 1 atm

#	Name	Isotope labeling	Concentration
9	Class I RNA aptamer to GTP	[U-15N; U-13C]	3.3 mM
10	GUANOSINE-5'-TRIPHOSPHATE		3.63 mM
11	phosphate buffer		10 %
12	D2O		5 %
13	H2O		95 %

Sample #4

Pressure 1 atm

#	Name	Isotope labeling	Concentration
14	Class I RNA aptamer to GTP	[U-15N; U-13C]	0.9 mM
15	GUANOSINE-5'-TRIPHOSPHATE		0.99 mM
16	phosphate buffer		10 %
17	D2O		5 %
18	H2O		95 %

4 DQF-COSY

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm

#	Name	Isotope labeling	Concentration
1	Class I RNA aptamer to GTP	[U-15N; U-13C]	3.3 mM
2	GUANOSINE-5'-TRIPHOSPHATE		3.63 mM
3	phosphate buffer		10 %
4	D2O		99 %

Sample #2

Pressure 1 atm

#	Name	Isotope labeling	Concentration
5	Class I RNA aptamer to GTP	[U-15N; U-13C]	0.9 mM
6	GUANOSINE-5'-TRIPHOSPHATE		0.99 mM
7	phosphate buffer		10 %
8	D2O		99 %

Sample #3

Pressure 1 atm

#	Name	Isotope labeling	Concentration
9	Class I RNA aptamer to GTP	[U-15N; U-13C]	3.3 mM
10	GUANOSINE-5'-TRIPHOSPHATE		3.63 mM
11	phosphate buffer		10 %
12	D2O		5 %
13	H2O		95 %

Sample #4

Pressure 1 atm

#	Name	Isotope labeling	Concentration
14	Class I RNA aptamer to GTP	[U-15N; U-13C]	0.9 mM
15	GUANOSINE-5'-TRIPHOSPHATE		0.99 mM
16	phosphate buffer		10 %
17	D2O		5 %
18	H2O		95 %

5 2D 1H decoupled 13C-1H CT HSQC

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm

#	Name	Isotope labeling	Concentration
1	Class I RNA aptamer to GTP	[U-15N; U-13C]	3.3 mM
2	GUANOSINE-5'-TRIPHOSPHATE		3.63 mM
3	phosphate buffer		10 %
4	D2O		99 %

Sample #2

Pressure 1 atm

#	Name	Isotope labeling	Concentration
5	Class I RNA aptamer to GTP	[U-15N; U-13C]	0.9 mM
6	GUANOSINE-5'-TRIPHOSPHATE		0.99 mM
7	phosphate buffer		10 %
8	D2O		99 %

Sample #3

Pressure 1 atm

#	Name	Isotope labeling	Concentration
9	Class I RNA aptamer to GTP	[U-15N; U-13C]	3.3 mM
10	GUANOSINE-5'-TRIPHOSPHATE		3.63 mM
11	phosphate buffer		10 %
12	D2O		5 %
13	H2O		95 %

Sample #4

Pressure 1 atm

#	Name	Isotope labeling	Concentration
14	Class I RNA aptamer to GTP	[U-15N; U-13C]	0.9 mM
15	GUANOSINE-5'-TRIPHOSPHATE		0.99 mM
16	phosphate buffer		10 %
17	D2O		5 %
18	H2O		95 %

6 2D 13C-1H CT-TROSY

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm

#	Name	Isotope labeling	Concentration
1	Class I RNA aptamer to GTP	[U-15N; U-13C]	3.3 mM
2	GUANOSINE-5'-TRIPHOSPHATE		3.63 mM
3	phosphate buffer		10 %
4	D2O		99 %

Sample #2

Pressure 1 atm

#	Name	Isotope labeling	Concentration
5	Class I RNA aptamer to GTP	[U-15N; U-13C]	0.9 mM
6	GUANOSINE-5'-TRIPHOSPHATE		0.99 mM
7	phosphate buffer		10 %
8	D2O		99 %

Sample #3

Pressure 1 atm

#	Name	Isotope labeling	Concentration
9	Class I RNA aptamer to GTP	[U-15N; U-13C]	3.3 mM
10	GUANOSINE-5'-TRIPHOSPHATE		3.63 mM
11	phosphate buffer		10 %
12	D2O		5 %
13	H2O		95 %

Sample #4

Pressure 1 atm

#	Name	Isotope labeling	Concentration
14	Class I RNA aptamer to GTP	[U-15N; U-13C]	0.9 mM
15	GUANOSINE-5'-TRIPHOSPHATE		0.99 mM
16	phosphate buffer		10 %
17	D2O		5 %
18	H2O		95 %

nullKeywords aptamer, RNA

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Found 1 Document (1.623 seconds)

BMRB: 6814

Sample

Sample

Related entities 1. Class I RNA aptamer to GTP, : 1 entities Detail

Experiments performed 6 experiments Detail

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