BMRB: 6815

NMR structure of Retrocyclin-2 in SDS

Authors

Daly, N.L., Chen, Y., Rosengren, K.J., Marx, U.C., Phillips, M.L., Waring, A.J., Wang, W., Lehrer, R.I., Craik, D.J.

Assembly

Retrocyclin-2

Entity

1. Retrocyclin-2 (polymer, Thiol state: all disulfide bound), 18 monomers, 2041.587 Da Detail

RRICRCICGR GICRCICG

Formula weight

2041.587 Da

Entity Connection

disulfide 3 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS4:SG	1:CYS17:SG
2	disulfide	sing	1:CYS6:SG	1:CYS15:SG
3	disulfide	sing	1:CYS8:SG	1:CYS13:SG

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 76.4 %, Completeness (bb): 100.0 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H
All	76.4 % (81 of 106)	76.4 % (81 of 106)
Backbone	100.0 % (39 of 39)	100.0 % (39 of 39)
Sidechain	62.7 % (42 of 67)	62.7 % (42 of 67)
Methyl	50.0 % (4 of 8)	50.0 % (4 of 8)

1. Retrocyclin-2

RRICRCICGR GICRCICG

Show sample condition

Sample

Pressure 1 atm, Temperature 310 K, pH 3.5

#	Name	Isotope labeling	Type	Concentration
1	Retrocyclin-2			0.6 mM
2	SDS			100 mM
3	H2O			90 %
4	D2O			10 %

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 2ATG, Strand ID: A Detail

---

Release date

2005-09-28

Citation

Retrocyclin-2: structural analysis of a potent anti-HIV theta-defensin
Daly, N.L., Chen, Y., Rosengren, K.J., Marx, U.C., Phillips, M.L., Waring, A.J., Wang, W., Lehrer, R.I., Craik, D.J.
Biochemistry (2007), 46, 9920-9928, PubMed 17685559 , DOI 10.1021/bi700720e , Abstract

Related entities 1. Retrocyclin-2, : 1 : 1 : 5 entities Detail

Experiments performed 4 experiments Detail