BMRBj - BMREntryMaster

Found 1 Document (1.817 seconds)

BMRB: 6816

2AHQ

Solution structure of the C-terminal RpoN domain of aquifex aeolicus SIGMA-54 shows similarity to SIGMA-70

Authors

Doucleff, M., Malak, L.T., Pelton, J.G., Wemmer, D.E.

Assembly

RNA polymerase sigma factor RpoN

Entity

1. RNA polymerase sigma factor RpoN (polymer, Thiol state: not present), 76 monomers, 8949.237 Da Detail

TYSLRTFFVR ESAEGLTQGE LMKLIKEIVE NEDKRKPYSD QEIANILKEK GFKVARRTVA KYREMLGIPS SRERRI

Formula weight

8949.237 Da

Source organism

Aquifex aeolicus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.8 %, Completeness: 72.1 %, Completeness (bb): 73.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	72.1 % (677 of 939)	74.8 % (374 of 500)	64.5 % (233 of 361)	89.7 % (70 of 78)
Backbone	73.2 % (331 of 452)	84.4 % (130 of 154)	61.2 % (137 of 224)	86.5 % (64 of 74)
Sidechain	68.9 % (385 of 559)	66.8 % (231 of 346)	71.8 % (150 of 209)	100.0 % (4 of 4)
Aromatic	40.7 % (22 of 54)	81.5 % (22 of 27)	0.0 % (0 of 27)
Methyl	87.5 % (70 of 80)	87.5 % (35 of 40)	87.5 % (35 of 40)

1. RNA polymerase sigma factor RpoN

TYSLRTFFVR ESAEGLTQGE LMKLIKEIVE NEDKRKPYSD QEIANILKEK GFKVARRTVA KYREMLGIPS SRERRI

Show sample condition

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	RNA polymerase sigma factor RpoN	[U-15N]	0.8 mM
2	Hepes		50 mM
3	NaCl		250 mM
4	EDTA		1 mM
5	H20		90 %
6	D20		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
7	RNA polymerase sigma factor RpoN	[U-13C; U-15N]	0.8 mM
8	Hepes		50 mM
9	NaCl		250 mM
10	EDTA		1 mM
11	H20		90 %
12	D20		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
13	RNA polymerase sigma factor RpoN	[U-15N]	0.8 mM
14	Hepes		50 mM
15	NaCl		250 mM
16	EDTA		1 mM
17	H20		5 %
18	D20		95 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
19	RNA polymerase sigma factor RpoN	[U-13C; U-15N]	0.8 mM
20	Hepes		50 mM
21	NaCl		250 mM
22	EDTA		1 mM
23	H20		90 %
24	D20		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	H2O	protons	4.77 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: -0.34 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	H2O	protons	4.77 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: -0.34 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	H2O	protons	4.77 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2AHQ, Strand ID: A Detail

Release date

2007-01-28

Citation

The C-terminal RpoN domain of sigma54 forms an unpredicted helix-turn-helix motif similar to domains of sigma70
Doucleff, M., Malak, L.T., Pelton, J.G., Wemmer, D.E.
J. Biol. Chem. (2005), 280, 41530-41536, PubMed 16210314 , DOI 10.1074/jbc.M509010200 , Abstract

Related entities 1. RNA polymerase sigma factor RpoN, : 1 : 1 : 2 : 26 entities Detail

Experiments performed 6 experiments Detail

1 3D 15N-separated NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	RNA polymerase sigma factor RpoN	[U-15N]	0.8 mM
2	Hepes		50 mM
3	NaCl		250 mM
4	EDTA		1 mM
5	H20		90 %
6	D20		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
7	RNA polymerase sigma factor RpoN	[U-13C; U-15N]	0.8 mM
8	Hepes		50 mM
9	NaCl		250 mM
10	EDTA		1 mM
11	H20		90 %
12	D20		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
13	RNA polymerase sigma factor RpoN	[U-15N]	0.8 mM
14	Hepes		50 mM
15	NaCl		250 mM
16	EDTA		1 mM
17	H20		5 %
18	D20		95 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
19	RNA polymerase sigma factor RpoN	[U-13C; U-15N]	0.8 mM
20	Hepes		50 mM
21	NaCl		250 mM
22	EDTA		1 mM
23	H20		90 %
24	D20		10 %

2 3D N15-seperated TOCSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	RNA polymerase sigma factor RpoN	[U-15N]	0.8 mM
2	Hepes		50 mM
3	NaCl		250 mM
4	EDTA		1 mM
5	H20		90 %
6	D20		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
7	RNA polymerase sigma factor RpoN	[U-13C; U-15N]	0.8 mM
8	Hepes		50 mM
9	NaCl		250 mM
10	EDTA		1 mM
11	H20		90 %
12	D20		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
13	RNA polymerase sigma factor RpoN	[U-15N]	0.8 mM
14	Hepes		50 mM
15	NaCl		250 mM
16	EDTA		1 mM
17	H20		5 %
18	D20		95 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
19	RNA polymerase sigma factor RpoN	[U-13C; U-15N]	0.8 mM
20	Hepes		50 mM
21	NaCl		250 mM
22	EDTA		1 mM
23	H20		90 %
24	D20		10 %

3 HNCA-J

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	RNA polymerase sigma factor RpoN	[U-15N]	0.8 mM
2	Hepes		50 mM
3	NaCl		250 mM
4	EDTA		1 mM
5	H20		90 %
6	D20		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
7	RNA polymerase sigma factor RpoN	[U-13C; U-15N]	0.8 mM
8	Hepes		50 mM
9	NaCl		250 mM
10	EDTA		1 mM
11	H20		90 %
12	D20		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
13	RNA polymerase sigma factor RpoN	[U-15N]	0.8 mM
14	Hepes		50 mM
15	NaCl		250 mM
16	EDTA		1 mM
17	H20		5 %
18	D20		95 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
19	RNA polymerase sigma factor RpoN	[U-13C; U-15N]	0.8 mM
20	Hepes		50 mM
21	NaCl		250 mM
22	EDTA		1 mM
23	H20		90 %
24	D20		10 %

4 HCCH-TOCSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	RNA polymerase sigma factor RpoN	[U-15N]	0.8 mM
2	Hepes		50 mM
3	NaCl		250 mM
4	EDTA		1 mM
5	H20		90 %
6	D20		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
7	RNA polymerase sigma factor RpoN	[U-13C; U-15N]	0.8 mM
8	Hepes		50 mM
9	NaCl		250 mM
10	EDTA		1 mM
11	H20		90 %
12	D20		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
13	RNA polymerase sigma factor RpoN	[U-15N]	0.8 mM
14	Hepes		50 mM
15	NaCl		250 mM
16	EDTA		1 mM
17	H20		5 %
18	D20		95 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
19	RNA polymerase sigma factor RpoN	[U-13C; U-15N]	0.8 mM
20	Hepes		50 mM
21	NaCl		250 mM
22	EDTA		1 mM
23	H20		90 %
24	D20		10 %

5 3D 13C-separated NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	RNA polymerase sigma factor RpoN	[U-15N]	0.8 mM
2	Hepes		50 mM
3	NaCl		250 mM
4	EDTA		1 mM
5	H20		90 %
6	D20		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
7	RNA polymerase sigma factor RpoN	[U-13C; U-15N]	0.8 mM
8	Hepes		50 mM
9	NaCl		250 mM
10	EDTA		1 mM
11	H20		90 %
12	D20		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
13	RNA polymerase sigma factor RpoN	[U-15N]	0.8 mM
14	Hepes		50 mM
15	NaCl		250 mM
16	EDTA		1 mM
17	H20		5 %
18	D20		95 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
19	RNA polymerase sigma factor RpoN	[U-13C; U-15N]	0.8 mM
20	Hepes		50 mM
21	NaCl		250 mM
22	EDTA		1 mM
23	H20		90 %
24	D20		10 %

6 2D C13-HSQC

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	RNA polymerase sigma factor RpoN	[U-15N]	0.8 mM
2	Hepes		50 mM
3	NaCl		250 mM
4	EDTA		1 mM
5	H20		90 %
6	D20		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
7	RNA polymerase sigma factor RpoN	[U-13C; U-15N]	0.8 mM
8	Hepes		50 mM
9	NaCl		250 mM
10	EDTA		1 mM
11	H20		90 %
12	D20		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
13	RNA polymerase sigma factor RpoN	[U-15N]	0.8 mM
14	Hepes		50 mM
15	NaCl		250 mM
16	EDTA		1 mM
17	H20		5 %
18	D20		95 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
19	RNA polymerase sigma factor RpoN	[U-13C; U-15N]	0.8 mM
20	Hepes		50 mM
21	NaCl		250 mM
22	EDTA		1 mM
23	H20		90 %
24	D20		10 %

nullKeywords RNA polymerase, Sigma-54, sigma factors, solution structure, transcription

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Found 1 Document (1.817 seconds)

BMRB: 6816

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. RNA polymerase sigma factor RpoN, : 1 : 1 : 2 : 26 entities Detail

Experiments performed 6 experiments Detail

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