BMRB: 6817

Structure of cyclic conotoxin MII-7

Authors

Clark, R.J., Fischer, H., Dempster, L., Daly, N.L., Rosengren, K.J., Nevin, S., Meunier, F.A., Adams, D.J., Craik, D.J.

Assembly

Alpha-conotoxin MII

Entity

1. Alpha-conotoxin MII (polymer, Thiol state: all disulfide bound), 23 monomers, 2157.391 Da Detail

GCCSNPVCHL EHSNLCGAGG AAG

Formula weight

2157.391 Da

Entity Connection

disulfide 2 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS2:SG	1:CYS8:SG
2	disulfide	sing	1:CYS3:SG	1:CYS16:SG

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.1 %, Completeness (bb): 100.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	97.1 % (101 of 104)	97.1 % (101 of 104)
Backbone	100.0 % (50 of 50)	100.0 % (50 of 50)
Sidechain	94.4 % (51 of 54)	94.4 % (51 of 54)
Aromatic	100.0 % (4 of 4)	100.0 % (4 of 4)
Methyl	100.0 % (9 of 9)	100.0 % (9 of 9)

1. Alpha-conotoxin MII

GCCSNPVCHL EHSNLCGAGG AAG

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 3.5

#	Name	Isotope labeling	Type	Concentration
1	Alpha-conotoxin MII			1 mM
2	H2O			90 %
3	D2O			10 %

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Calculated from 20 models in PDB: 2AK0, Strand ID: A Detail

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Release date

2005-11-20

Citation

Engineering stable peptide toxins by means of backbone cyclization: stabilization of the alpha-conotoxin MII
Clark, R.J., Fischer, H., Dempster, L., Daly, N.L., Rosengren, K.J., Nevin, S., Meunier, F.A., Adams, D.J., Craik, D.J.
Proc. Natl. Acad. Sci. U. S. A. (2005), 102, 13767-13772, PubMed 16162671 , DOI 10.1073/pnas.0504613102 , Abstract

Related entities 1. Alpha-conotoxin MII, : 1 : 7 entities Detail

Experiments performed 4 experiments Detail