BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	93.2 % (1048 of 1125)	95.4 % (566 of 593)	89.4 % (388 of 434)	95.9 % (94 of 98)
Backbone	95.3 % (547 of 574)	96.5 % (192 of 199)	94.3 % (264 of 280)	95.8 % (91 of 95)
Sidechain	92.0 % (588 of 639)	94.9 % (374 of 394)	87.2 % (211 of 242)	100.0 % (3 of 3)
Aromatic	51.7 % (30 of 58)	89.7 % (26 of 29)	13.8 % (4 of 29)
Methyl	94.3 % (83 of 88)	93.2 % (41 of 44)	95.5 % (42 of 44)

1. Caltractin

GSMSYKAKTV VSARRDQKKG RVGLTEEQKQ EIREAFDLFD TDGSGTIDAK ELKVAMRALG FEPKKEEIKK MISEIDKDGS GTIDFEEFLT MMTAKM

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
1	Caltractin	1.0 ~ 2.0 mM
2	Tris-d11	25 mM
3	CaCl2	5.0 ~ 10.0 mM
4	H2O	90 %
5	D2O	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.45 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2AMI, Strand ID: A Detail

Release date

2006-11-05

Citation

Structure of the N-terminal calcium sensor domain of centrin reveals the biochemical basis for domain-specific function
Hu, H.T., Fagan, P.A., Bunick, C.G., Sheehan, J.H., Chazin, W.J.
J. Biol. Chem. (2006), 281, 2876-2881, PubMed 16317001 , DOI 10.1074/jbc.M509886200 , Abstract

Related entities 1. Caltractin, : 1 : 2 : 78 entities Detail

Interaction partners 1. Caltractin, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 4 experiments Detail

1 2D NOESY

Instrument

Model Bruker DRX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
1	Caltractin	1.0 ~ 2.0 mM
2	Tris-d11	25 mM
3	CaCl2	5.0 ~ 10.0 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Caltractin	[U-15N]	1.0 ~ 2.0 mM
7	Tris-d11		25 mM
8	CaCl2		5.0 ~ 10.0 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
11	Caltractin	[U-13C]	1.0 ~ 2.0 mM
12	Tris-d11		25 mM
13	CaCl2		5.0 ~ 10.0 mM
14	H2O		90 %
15	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
16	Caltractin	[U-13C; U-15N]	1.0 ~ 2.0 mM
17	Tris-d11		25 mM
18	CaCl2		5.0 ~ 10.0 mM
19	H2O		90 %
20	D2O		10 %

Sample #5

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
21	Caltractin	[U-10% 13C]	1.0 ~ 2.0 mM
22	Tris-d11		25 mM
23	CaCl2		5.0 ~ 10.0 mM
24	H2O		90 %
25	D2O		10 %

2 3D 15N-NOESY-HSQC

Instrument

Model Bruker DRX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
1	Caltractin	1.0 ~ 2.0 mM
2	Tris-d11	25 mM
3	CaCl2	5.0 ~ 10.0 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Caltractin	[U-15N]	1.0 ~ 2.0 mM
7	Tris-d11		25 mM
8	CaCl2		5.0 ~ 10.0 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
11	Caltractin	[U-13C]	1.0 ~ 2.0 mM
12	Tris-d11		25 mM
13	CaCl2		5.0 ~ 10.0 mM
14	H2O		90 %
15	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
16	Caltractin	[U-13C; U-15N]	1.0 ~ 2.0 mM
17	Tris-d11		25 mM
18	CaCl2		5.0 ~ 10.0 mM
19	H2O		90 %
20	D2O		10 %

Sample #5

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
21	Caltractin	[U-10% 13C]	1.0 ~ 2.0 mM
22	Tris-d11		25 mM
23	CaCl2		5.0 ~ 10.0 mM
24	H2O		90 %
25	D2O		10 %

3 3D 13C-NOESY-HSQC

Instrument

Model Bruker DRX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
1	Caltractin	1.0 ~ 2.0 mM
2	Tris-d11	25 mM
3	CaCl2	5.0 ~ 10.0 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Caltractin	[U-15N]	1.0 ~ 2.0 mM
7	Tris-d11		25 mM
8	CaCl2		5.0 ~ 10.0 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
11	Caltractin	[U-13C]	1.0 ~ 2.0 mM
12	Tris-d11		25 mM
13	CaCl2		5.0 ~ 10.0 mM
14	H2O		90 %
15	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
16	Caltractin	[U-13C; U-15N]	1.0 ~ 2.0 mM
17	Tris-d11		25 mM
18	CaCl2		5.0 ~ 10.0 mM
19	H2O		90 %
20	D2O		10 %

Sample #5

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
21	Caltractin	[U-10% 13C]	1.0 ~ 2.0 mM
22	Tris-d11		25 mM
23	CaCl2		5.0 ~ 10.0 mM
24	H2O		90 %
25	D2O		10 %

4 4D 13C-HMQC-NOESY-HMQC

Instrument

Model Bruker DRX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
1	Caltractin	1.0 ~ 2.0 mM
2	Tris-d11	25 mM
3	CaCl2	5.0 ~ 10.0 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Caltractin	[U-15N]	1.0 ~ 2.0 mM
7	Tris-d11		25 mM
8	CaCl2		5.0 ~ 10.0 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
11	Caltractin	[U-13C]	1.0 ~ 2.0 mM
12	Tris-d11		25 mM
13	CaCl2		5.0 ~ 10.0 mM
14	H2O		90 %
15	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
16	Caltractin	[U-13C; U-15N]	1.0 ~ 2.0 mM
17	Tris-d11		25 mM
18	CaCl2		5.0 ~ 10.0 mM
19	H2O		90 %
20	D2O		10 %

Sample #5

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
21	Caltractin	[U-10% 13C]	1.0 ~ 2.0 mM
22	Tris-d11		25 mM
23	CaCl2		5.0 ~ 10.0 mM
24	H2O		90 %
25	D2O		10 %

nullKeywords Calcium Sensor, Calmodulin, Centrin, EF-Hand Calcium Binding Protein, Four-Helix Bundle

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BMRB: 6820

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Caltractin, : 1 : 2 : 78 entities Detail

Interaction partners 1. Caltractin, : 1 interactors Detail

Experiments performed 4 experiments Detail

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Sample #5

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