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Solution structure of Calmodulin-like Skin Protein C terminal domain
Authors
Babini, E., Bertini, I., Capozzi, F., Chirivino, E., Luchinat, C.
Assembly
Calmodulin-like protein 5
Entity
1. Calmodulin-like protein 5 (polymer, Thiol state: not present), 71 monomers, 7961.674 Da Detail

ARAGLEDLQV AFRAFDQDGD GHITVDELRR AMAGLGQPLP QEELDAMIRE ADVDQDGRVN YEEFARMLAQ E


Formula weight
7961.674 Da
Source organism
Homo sapiens
Exptl. method
NMR
Refine. method
torsion angle dynamics, coupled with simulated annealing, followed by restrained energy minimization
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 98.6 %, Completeness: 87.1 %, Completeness (bb): 94.4 % Detail

Polymer type: polypeptide(L)

Total1H15N
All87.1 % (425 of 488)87.6 % (361 of 412)84.2 % (64 of 76)
Backbone94.4 % (203 of 215)95.9 % (140 of 146)91.3 % (63 of 69)
Sidechain81.3 % (222 of 273)83.1 % (221 of 266)14.3 % (1 of 7)
Aromatic57.1 % (12 of 21)57.1 % (12 of 21)
Methyl86.5 % (32 of 37)86.5 % (32 of 37)

1. Calmodulin-like protein 5

ARAGLEDLQV AFRAFDQDGD GHITVDELRR AMAGLGQPLP QEELDAMIRE ADVDQDGRVN YEEFARMLAQ E

Sample

Pressure 1 atm, Temperature 298 K, pH 6.8


#NameIsotope labelingTypeConcentration
1Calmodulin-like protein 5[U-15N]2 mM
2NaCl100 mM
3H2O90 %
4D2O10 %

Protein Blocks Logo
Calculated from 20 models in PDB: 2B1U, Strand ID: A Detail


Release date
2006-06-26
Citation
A structural and dynamic characterization of the EF-hand protein CLSP
Babini, E., Bertini, I., Capozzi, F., Chirivino, E., Luchinat, C.
Structure (2006), 14, 1029-1038, PubMed 16765896 , DOI 10.1016/j.str.2006.04.004 ,
Related entities 1. Calmodulin-like protein 5, : 1 : 2 : 559 entities Detail
Interaction partners 1. Calmodulin-like protein 5, : 13 interactors Detail
Experiments performed 5 experiments Detail
nullKeywords Backbone dynamic, Calmodulin-like skin protein, CLSP, NMR, Solution structure, Protein Structure Initiative, PSI, SPINE, Structural Genomics, Structural Proteomics in Europe