BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	73.3 % (1109 of 1513)	72.4 % (568 of 785)	74.2 % (439 of 592)	75.0 % (102 of 136)
Backbone	77.3 % (580 of 750)	77.4 % (195 of 252)	76.7 % (289 of 377)	79.3 % (96 of 121)
Sidechain	70.7 % (626 of 886)	70.0 % (373 of 533)	73.1 % (247 of 338)	40.0 % (6 of 15)
Aromatic	57.7 % (60 of 104)	57.7 % (30 of 52)	58.8 % (30 of 51)	0.0 % (0 of 1)
Methyl	74.4 % (125 of 168)	75.0 % (63 of 84)	73.8 % (62 of 84)

1. Caspase recruitment domain protein 4

GAMESHPHIQ LLKSNRELLV THIRNTQCLV DNLLKNDYFS AEDAEIVCAC PTQPDKVRKI LDLVQSKGEE VSEFFLYLLQ QLADAYVDLR PWLLEIGFSP SLLTQSKVVV NTDPVSRYTQ QLRHHLG

Show sample condition

Sample

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
1	Caspase recruitment domain protein 4	[U-95% 13C; U-90% 15N]	1.4 mM
2	phosphate buffer		25 mM
3	NaCl		100 mM
4	DTT		5 mM
5	H2O		95 %
6	D2O		5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.18 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.18 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.86 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2B1W, Strand ID: A Detail

Release date

2006-11-05

Citation

Solution structure of NOD1 CARD and mutational analysis of its interaction with the CARD of downstream kinase RICK
Manon, F., Favier, A., Nunez, G., Simorre, J.P., Cusack, S.
J. Mol. Biol. (2007), 365, 160-174, PubMed 17054981 , DOI 10.1016/j.jmb.2006.09.067 , Abstract

Related entities 1. Caspase recruitment domain protein 4, : 2 : 11 entities Detail

Interaction partners 1. Caspase recruitment domain protein 4, : 27 interactors Detail

More details for 27 interactors identified by 8 citations

Experiments performed 8 experiments Detail

1 HNCO

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
1	Caspase recruitment domain protein 4	[U-95% 13C; U-90% 15N]	1.4 mM
2	phosphate buffer		25 mM
3	NaCl		100 mM
4	DTT		5 mM
5	H2O		95 %
6	D2O		5 %

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
7	Caspase recruitment domain protein 4	[U-90% 15N]	1.4 mM
8	phosphate buffer		25 mM
9	NaCl		100 mM
10	DTT		5 mM
11	H2O		95 %
12	D2O		5 %

2 HNCA

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
1	Caspase recruitment domain protein 4	[U-95% 13C; U-90% 15N]	1.4 mM
2	phosphate buffer		25 mM
3	NaCl		100 mM
4	DTT		5 mM
5	H2O		95 %
6	D2O		5 %

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
7	Caspase recruitment domain protein 4	[U-90% 15N]	1.4 mM
8	phosphate buffer		25 mM
9	NaCl		100 mM
10	DTT		5 mM
11	H2O		95 %
12	D2O		5 %

3 HNCACB

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
1	Caspase recruitment domain protein 4	[U-95% 13C; U-90% 15N]	1.4 mM
2	phosphate buffer		25 mM
3	NaCl		100 mM
4	DTT		5 mM
5	H2O		95 %
6	D2O		5 %

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
7	Caspase recruitment domain protein 4	[U-90% 15N]	1.4 mM
8	phosphate buffer		25 mM
9	NaCl		100 mM
10	DTT		5 mM
11	H2O		95 %
12	D2O		5 %

4 CBCA(CO)NH

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
1	Caspase recruitment domain protein 4	[U-95% 13C; U-90% 15N]	1.4 mM
2	phosphate buffer		25 mM
3	NaCl		100 mM
4	DTT		5 mM
5	H2O		95 %
6	D2O		5 %

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
7	Caspase recruitment domain protein 4	[U-90% 15N]	1.4 mM
8	phosphate buffer		25 mM
9	NaCl		100 mM
10	DTT		5 mM
11	H2O		95 %
12	D2O		5 %

5 HN(CA)CO

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
1	Caspase recruitment domain protein 4	[U-95% 13C; U-90% 15N]	1.4 mM
2	phosphate buffer		25 mM
3	NaCl		100 mM
4	DTT		5 mM
5	H2O		95 %
6	D2O		5 %

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
7	Caspase recruitment domain protein 4	[U-90% 15N]	1.4 mM
8	phosphate buffer		25 mM
9	NaCl		100 mM
10	DTT		5 mM
11	H2O		95 %
12	D2O		5 %

6 H(C)CH-TOCSY3D 13C-separated NOESY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
1	Caspase recruitment domain protein 4	[U-95% 13C; U-90% 15N]	1.4 mM
2	phosphate buffer		25 mM
3	NaCl		100 mM
4	DTT		5 mM
5	H2O		95 %
6	D2O		5 %

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
7	Caspase recruitment domain protein 4	[U-90% 15N]	1.4 mM
8	phosphate buffer		25 mM
9	NaCl		100 mM
10	DTT		5 mM
11	H2O		95 %
12	D2O		5 %

7 3D_15N-separated NOESY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
1	Caspase recruitment domain protein 4	[U-95% 13C; U-90% 15N]	1.4 mM
2	phosphate buffer		25 mM
3	NaCl		100 mM
4	DTT		5 mM
5	H2O		95 %
6	D2O		5 %

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
7	Caspase recruitment domain protein 4	[U-90% 15N]	1.4 mM
8	phosphate buffer		25 mM
9	NaCl		100 mM
10	DTT		5 mM
11	H2O		95 %
12	D2O		5 %

8 3D methyl selected NOESY-HSQC

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
1	Caspase recruitment domain protein 4	[U-95% 13C; U-90% 15N]	1.4 mM
2	phosphate buffer		25 mM
3	NaCl		100 mM
4	DTT		5 mM
5	H2O		95 %
6	D2O		5 %

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7 (±0.1)

#	Name	Isotope labeling	Concentration
7	Caspase recruitment domain protein 4	[U-90% 15N]	1.4 mM
8	phosphate buffer		25 mM
9	NaCl		100 mM
10	DTT		5 mM
11	H2O		95 %
12	D2O		5 %

nullKeywords CARD4, Caspase recruitment domain, greek key, inflammation, NF-kB, six-helix bundle

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.156 seconds)

BMRB: 6843

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Caspase recruitment domain protein 4, : 2 : 11 entities Detail

Interaction partners 1. Caspase recruitment domain protein 4, : 27 interactors Detail

Experiments performed 8 experiments Detail

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2