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NMR structural analysis of Nop10p from Saccharomyces cerevisiae
Authors
Hamma, T., Reichow, S., Varani, G., Ferre-D'Amare, A.
Assembly
H/ACA ribonucleoprotein complex subunit 3
Entity
1. H/ACA ribonucleoprotein complex subunit 3 (polymer, Thiol state: not present), 63 monomers, 7327.268 Da Detail

HLMYTLGPDG KRIYTLKKVT ESGEITKSAH PARFSPDDKY SRQRVTLKKR FGLVPGQHHH HHH


Formula weight
7327.268 Da
Source organism
Saccharomyces cerevisiae
Exptl. method
NMR
Refine. method
torsion angle dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 90.5 %, Completeness: 85.5 %, Completeness (bb): 87.6 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All85.5 % (651 of 761)89.3 % (358 of 401)79.9 % (239 of 299)88.5 % (54 of 61)
Backbone87.6 % (324 of 370)89.8 % (114 of 127)85.9 % (158 of 184)88.1 % (52 of 59)
Sidechain84.4 % (379 of 449)89.1 % (244 of 274)76.9 % (133 of 173)100.0 % (2 of 2)
Aromatic32.9 % (25 of 76)55.3 % (21 of 38)10.5 % (4 of 38)
Methyl100.0 % (54 of 54)100.0 % (27 of 27)100.0 % (27 of 27)

1. H/ACA ribonucleoprotein complex subunit 3

HLMYTLGPDG KRIYTLKKVT ESGEITKSAH PARFSPDDKY SRQRVTLKKR FGLVPGQHHH HHH

Sample

Pressure 1 atm, Temperature 298 K, pH 5.0


#NameIsotope labelingTypeConcentration
1H/ACA ribonucleoprotein complex subunit 31 mM
2Sodium Acetate50 mM
3KCl100 mM
4H2O90 %
5D2O10 %

LACS Plot; CA
Referencing offset: -0.11 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.11 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.02 ppm, Outliers: 2 Detail
LACS Plot; CO
Referencing offset: -1.02 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2AQA, Strand ID: A Detail


Release date
2005-12-27
Citation
The Cbf5-Nop10 complex is a molecular bracket that organizes box H/ACA RNPs
Hamma, T., Reichow, S.L., Varani, G., Ferre-D'Amare, A.R.
Nat. Struct. Mol. Biol. (2005), 12, 1101-1107, PubMed 16286935 , DOI 10.1038/nsmb1036 ,
Related entities 1. H/ACA ribonucleoprotein complex subunit 3, : 1 : 5 : 44 entities Detail
Interaction partners 1. H/ACA ribonucleoprotein complex subunit 3, : 18 interactors Detail
Experiments performed 3 experiments Detail
nullKeywords aNop10, Zinc-Ribbon