BMRB: 6849

Crassostrea gigas defensin

Authors

Gueguen, Y., Amaury, H., Aumelas, A., Garnier, J., Fievet, J., Escoubas, J., Bulet, P., Gonzales, M., Lelong, C., Favrel, P., Bachere, E.

Assembly

Grassostrea gigas defensin

Entity

1. Grassostrea gigas defensin (polymer, Thiol state: disulfide and other bound), 43 monomers, 4642.331 Da Detail

GFGCPGNQLK CNNHCKSISC RAGYCDAATL WLRCTCTDCN GKK

Formula weight

4642.331 Da

Entity Connection

disulfide 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS4:SG	1:CYS25:SG
2	disulfide	sing	1:CYS11:SG	1:CYS34:SG
3	disulfide	sing	1:CYS15:SG	1:CYS36:SG
4	disulfide	sing	1:CYS20:SG	1:CYS39:SG

Source organism

Crassostrea gigas

Exptl. method

NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.8 %, Completeness (bb): 98.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	98.8 % (239 of 242)	98.8 % (239 of 242)
Backbone	98.9 % (89 of 90)	98.9 % (89 of 90)
Sidechain	98.7 % (150 of 152)	98.7 % (150 of 152)
Aromatic	100.0 % (17 of 17)	100.0 % (17 of 17)
Methyl	100.0 % (14 of 14)	100.0 % (14 of 14)

1. Grassostrea gigas defensin

GFGCPGNQLK CNNHCKSISC RAGYCDAATL WLRCTCTDCN GKK

Show sample condition

Sample

Temperature 303 (±0.1) K, pH 3.25 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Grassostrea gigas defensin			0.8 (±0.1) mM

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Protein Blocks Logo

Calculated from 10 models in PDB: 2B68, Strand ID: A Detail

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Release date

2007-01-28

Citation

Characterization of a defensin from the oyster Crassostrea gigas. Recombinant production, folding, solution structure, antimicrobial activities, and gene expression
Gueguen, Y., Herpin, A., Aumelas, A., Garnier, J., Fievet, J., Escoubas, J., Bulet, P., Gonzalez, M., Lelong, C., Favrel, P., Bachere, E.
J. Biol. Chem. (2006), 281, 313-323, PubMed 16246846 , DOI 10.1074/jbc.M510850200 , Abstract

Related entities 1. Grassostrea gigas defensin, : 1 : 15 entities Detail

Experiments performed 3 experiments Detail