BMRB: 6850

First FF domain of the PRP40 yeast protein

Authors

Gasch, A., Wiesner, S., Martin-Malpartida, P., Ramirez-Espain, X., Ruiz, L., Macias, M.J.

Assembly

PRP40 FF1

Entity

1. PRP40 FF1 (polymer, Thiol state: not present), 59 monomers, 7150.020 Da Detail

GAMEAEKEFI TMLKENQVDS TWSFSRIISE LGTRDPRYWM VDDDPLWKKE MFEKYLSNR

Formula weight

7150.02 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 79.9 %, Completeness (bb): 83.4 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	79.9 % (586 of 733)	87.0 % (336 of 386)	66.5 % (189 of 284)	96.8 % (61 of 63)
Backbone	83.4 % (292 of 350)	97.5 % (115 of 118)	69.1 % (121 of 175)	98.2 % (56 of 57)
Sidechain	79.5 % (350 of 440)	82.5 % (221 of 268)	74.7 % (124 of 166)	83.3 % (5 of 6)
Aromatic	41.5 % (34 of 82)	75.6 % (31 of 41)	0.0 % (0 of 38)	100.0 % (3 of 3)
Methyl	100.0 % (46 of 46)	100.0 % (23 of 23)	100.0 % (23 of 23)

1. PRP40 first FF domain

GAMEAEKEFI TMLKENQVDS TWSFSRIISE LGTRDPRYWM VDDDPLWKKE MFEKYLSNR

Show sample condition

Sample #1

Pressure 1 (±0) atm, Temperature 285 (±1) K, Details U-15N FF domain NaPhosphate buffer NaCl

#	Name	Isotope labeling	Type	Concentration
1	PRP40 first FF domain	[U-15N]		1 mM
2	Na Phosphate			20 mM
3	NaCl			100 mM
4	H2O			90 %
5	D2O			10 %

Sample #2

Pressure 1 (±0) atm, Temperature 285 (±1) K, Details U-13C, U-15N ff domain NaPhosphate buffer NaCl

#	Name	Isotope labeling	Type	Concentration
6	PRP40 first FF domain	[U-13C; U-15N]		1 mM
7	Na Phosphate			20 mM
8	NaCl			100 mM
9	H2O			90 %
10	D2O			10 %

Hide sample codition

LACS Plot; CA

Referencing offset: 2.1 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: 2.1 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: 0.17 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 12 models in PDB: 2B7E, Strand ID: A Detail

---

Release date

2007-01-28

Citation

The structure of Prp40 FF1 domain and its interaction with the crn-TPR1 motif of Clf1 gives a new insight into the binding mode of FF domains
Gasch, A., Wiesner, S., Martin-Malpartida, P., Ramirez-Espain, X., Ruiz, L., Macias, M.J.
J. Biol. Chem. (2006), 281, 356-364, PubMed 16253993 , DOI 10.1074/jbc.M508047200 , Abstract

Related entities 1. PRP40 FF1, : 1 : 2 : 5 entities Detail

Interaction partners 1. PRP40 FF1, : 44 interactors Detail

Experiments performed 2 experiments Detail