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BMRB: 6860

2GW6

Chemical shift assignments of the human ortholog of tRNA endonuclease subunt SEN15

Authors

Song, J., Markley, J.

Assembly

BC022030

Entity

1. BC022030 (polymer, Thiol state: all free), 123 monomers, 13774.66 Da Detail

SEDAWMGTHP KYLEMMELDI GDATQVYVAF LVYLDLMESK SWHEVNCVGL PELQLICLVG TEIEGEGLQT VVPTPITASL SHNRIREILK ASRKLQGDPD LPMSFTLAIV ESDSTIVYYK LTD

Formula weight

13774.66 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics,simulated annealing,distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 88.6 %, Completeness (bb): 98.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.6 % (1259 of 1421)	88.2 % (643 of 729)	86.8 % (492 of 567)	99.2 % (124 of 125)
Backbone	98.6 % (716 of 726)	98.8 % (244 of 247)	98.3 % (356 of 362)	99.1 % (116 of 117)
Sidechain	81.3 % (659 of 811)	82.8 % (399 of 482)	78.5 % (252 of 321)	100.0 % (8 of 8)
Aromatic	34.4 % (33 of 96)	60.4 % (29 of 48)	4.3 % (2 of 46)	100.0 % (2 of 2)
Methyl	93.4 % (155 of 166)	94.0 % (78 of 83)	92.8 % (77 of 83)

1. BC022030

SEDAWMGTHP KYLEMMELDI GDATQVYVAF LVYLDLMESK SWHEVNCVGL PELQLICLVG TEIEGEGLQT VVPTPITASL SHNRIREILK ASRKLQGDPD LPMSFTLAIV ESDSTIVYYK LTD

Show sample condition

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
2	Bis-Tris		buffer	10 (±0.5) mM
3	DTT		reducing agent	10 (±0.5) mM
4	NaCl		salt	100 (±5.0) mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
6	BC022030		protein	0.5 (±0.1) mM
7	Bis-Tris		buffer	10 (±0.5) mM
8	DTT		reducing agent	10 (±0.5) mM
9	NaCl		salt	100 (±5.0) mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 1.41 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2GW6, Strand ID: A, B Detail

Release date

2005-10-19

Citation

Three-dimensional structure determined for a subunit of human tRNA splicing endonuclease (Sen15) reveals a novel dimeric fold
Song, J., Markley, J.
J. Mol. Biol. (2007), 366, 155-164, PubMed 17166513 , DOI 10.1016/j.jmb.2006.11.024 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: Q8WW01 released on 2004-07-19
Title SEN15_HUMAN Entity tRNA-splicing endonuclease subunit Sen15

Related entities 1. BC022030, : 1 : 2 : 7 entities Detail

Interaction partners 1. BC022030, : 4 interactors Detail

More details for 4 interactors identified by 4 citations

Experiments performed 16 experiments Detail

1 1H,15N-HSQC

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
2	Bis-Tris		buffer	10 (±0.5) mM
3	DTT		reducing agent	10 (±0.5) mM
4	NaCl		salt	100 (±5.0) mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
6	BC022030		protein	0.5 (±0.1) mM
7	Bis-Tris		buffer	10 (±0.5) mM
8	DTT		reducing agent	10 (±0.5) mM
9	NaCl		salt	100 (±5.0) mM

2 1H,13C-HSQC

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
2	Bis-Tris		buffer	10 (±0.5) mM
3	DTT		reducing agent	10 (±0.5) mM
4	NaCl		salt	100 (±5.0) mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
6	BC022030		protein	0.5 (±0.1) mM
7	Bis-Tris		buffer	10 (±0.5) mM
8	DTT		reducing agent	10 (±0.5) mM
9	NaCl		salt	100 (±5.0) mM

3 HNCACB

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
2	Bis-Tris		buffer	10 (±0.5) mM
3	DTT		reducing agent	10 (±0.5) mM
4	NaCl		salt	100 (±5.0) mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
6	BC022030		protein	0.5 (±0.1) mM
7	Bis-Tris		buffer	10 (±0.5) mM
8	DTT		reducing agent	10 (±0.5) mM
9	NaCl		salt	100 (±5.0) mM

4 CBCACONH

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
2	Bis-Tris		buffer	10 (±0.5) mM
3	DTT		reducing agent	10 (±0.5) mM
4	NaCl		salt	100 (±5.0) mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
6	BC022030		protein	0.5 (±0.1) mM
7	Bis-Tris		buffer	10 (±0.5) mM
8	DTT		reducing agent	10 (±0.5) mM
9	NaCl		salt	100 (±5.0) mM

5 HCCHTOCSY

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
2	Bis-Tris		buffer	10 (±0.5) mM
3	DTT		reducing agent	10 (±0.5) mM
4	NaCl		salt	100 (±5.0) mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
6	BC022030		protein	0.5 (±0.1) mM
7	Bis-Tris		buffer	10 (±0.5) mM
8	DTT		reducing agent	10 (±0.5) mM
9	NaCl		salt	100 (±5.0) mM

6 CCONH

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
2	Bis-Tris		buffer	10 (±0.5) mM
3	DTT		reducing agent	10 (±0.5) mM
4	NaCl		salt	100 (±5.0) mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
6	BC022030		protein	0.5 (±0.1) mM
7	Bis-Tris		buffer	10 (±0.5) mM
8	DTT		reducing agent	10 (±0.5) mM
9	NaCl		salt	100 (±5.0) mM

7 HCCONH

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
2	Bis-Tris		buffer	10 (±0.5) mM
3	DTT		reducing agent	10 (±0.5) mM
4	NaCl		salt	100 (±5.0) mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
6	BC022030		protein	0.5 (±0.1) mM
7	Bis-Tris		buffer	10 (±0.5) mM
8	DTT		reducing agent	10 (±0.5) mM
9	NaCl		salt	100 (±5.0) mM

8 HNCO

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
2	Bis-Tris		buffer	10 (±0.5) mM
3	DTT		reducing agent	10 (±0.5) mM
4	NaCl		salt	100 (±5.0) mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
6	BC022030		protein	0.5 (±0.1) mM
7	Bis-Tris		buffer	10 (±0.5) mM
8	DTT		reducing agent	10 (±0.5) mM
9	NaCl		salt	100 (±5.0) mM

9 N15 T1

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
2	Bis-Tris		buffer	10 (±0.5) mM
3	DTT		reducing agent	10 (±0.5) mM
4	NaCl		salt	100 (±5.0) mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
6	BC022030		protein	0.5 (±0.1) mM
7	Bis-Tris		buffer	10 (±0.5) mM
8	DTT		reducing agent	10 (±0.5) mM
9	NaCl		salt	100 (±5.0) mM

10 N15 T2

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
2	Bis-Tris		buffer	10 (±0.5) mM
3	DTT		reducing agent	10 (±0.5) mM
4	NaCl		salt	100 (±5.0) mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
6	BC022030		protein	0.5 (±0.1) mM
7	Bis-Tris		buffer	10 (±0.5) mM
8	DTT		reducing agent	10 (±0.5) mM
9	NaCl		salt	100 (±5.0) mM

11 3D 15N NOESY

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
2	Bis-Tris		buffer	10 (±0.5) mM
3	DTT		reducing agent	10 (±0.5) mM
4	NaCl		salt	100 (±5.0) mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
6	BC022030		protein	0.5 (±0.1) mM
7	Bis-Tris		buffer	10 (±0.5) mM
8	DTT		reducing agent	10 (±0.5) mM
9	NaCl		salt	100 (±5.0) mM

12 3D 13C NOESY

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
2	Bis-Tris		buffer	10 (±0.5) mM
3	DTT		reducing agent	10 (±0.5) mM
4	NaCl		salt	100 (±5.0) mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
6	BC022030		protein	0.5 (±0.1) mM
7	Bis-Tris		buffer	10 (±0.5) mM
8	DTT		reducing agent	10 (±0.5) mM
9	NaCl		salt	100 (±5.0) mM

13 C13 filtered/edited NOESY

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
2	Bis-Tris		buffer	10 (±0.5) mM
3	DTT		reducing agent	10 (±0.5) mM
4	NaCl		salt	100 (±5.0) mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
6	BC022030		protein	0.5 (±0.1) mM
7	Bis-Tris		buffer	10 (±0.5) mM
8	DTT		reducing agent	10 (±0.5) mM
9	NaCl		salt	100 (±5.0) mM

14 HBCBCGCDCEHE

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
2	Bis-Tris		buffer	10 (±0.5) mM
3	DTT		reducing agent	10 (±0.5) mM
4	NaCl		salt	100 (±5.0) mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
6	BC022030		protein	0.5 (±0.1) mM
7	Bis-Tris		buffer	10 (±0.5) mM
8	DTT		reducing agent	10 (±0.5) mM
9	NaCl		salt	100 (±5.0) mM

15 HBCBCGCDHD

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
2	Bis-Tris		buffer	10 (±0.5) mM
3	DTT		reducing agent	10 (±0.5) mM
4	NaCl		salt	100 (±5.0) mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
6	BC022030		protein	0.5 (±0.1) mM
7	Bis-Tris		buffer	10 (±0.5) mM
8	DTT		reducing agent	10 (±0.5) mM
9	NaCl		salt	100 (±5.0) mM

16 HBACONH

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
2	Bis-Tris		buffer	10 (±0.5) mM
3	DTT		reducing agent	10 (±0.5) mM
4	NaCl		salt	100 (±5.0) mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	BC022030	[U-15N; U-13C]	protein	0.5 (±0.1) mM
6	BC022030		protein	0.5 (±0.1) mM
7	Bis-Tris		buffer	10 (±0.5) mM
8	DTT		reducing agent	10 (±0.5) mM
9	NaCl		salt	100 (±5.0) mM

null

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Found 1 Document (2.206 seconds)

BMRB: 6860

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. BC022030, : 1 : 2 : 7 entities Detail

Interaction partners 1. BC022030, : 4 interactors Detail

Experiments performed 16 experiments Detail

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