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Backbone 1H, 13C, and 15N Chemical Shift Assignments for Mengovirus Leader polypeptide
Authors
Cornilescu, C.C., Porter, F.W., Qin, Z., Lee, M.S., Palmenberg, A.C., Markley, J.L.
Assembly
Zinc finger domain of Mengovirus Leader polypeptide
Entity
1. zinc finger domain (polymer, Thiol state: all other bound), 35 monomers, 3966.452 Da Detail

GSTMATTMEQ EICAHSMTFE ECPKCSALQY RNGFY


2. ZN (non-polymer), 65.409 Da
Total weight
4031.8608 Da
Max. entity weight
3966.452 Da
Source organism
Mengo virus
Exptl. method
NMR
Refine. method
torsion angle dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 88.6 %, Completeness: 68.3 %, Completeness (bb): 76.9 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All68.3 % (267 of 391)67.8 % (137 of 202)66.4 % (101 of 152)78.4 % (29 of 37)
Backbone76.9 % (160 of 208)76.1 % (54 of 71)76.7 % (79 of 103)79.4 % (27 of 34)
Sidechain59.7 % (129 of 216)63.4 % (83 of 131)53.7 % (44 of 82)66.7 % (2 of 3)
Aromatic10.0 % (4 of 40)20.0 % (4 of 20) 0.0 % (0 of 20)
Methyl72.7 % (16 of 22)63.6 % (7 of 11)81.8 % (9 of 11)

1. zinc finger domain

GSTMATTMEQ EICAHSMTFE ECPKCSALQY RNGFY

Sample

Temperature 298 (±1) K, pH 7.0 (±0.1)


#NameIsotope labelingTypeConcentration
1zinc finger domain[U-13C; U-15N]0.5 mM
2NaPi100 mM

Protein Blocks Logo
Calculated from 20 models in PDB: 2BAI, Strand ID: A Detail


Release date
2005-12-15
Citation
Leader protein of encephalomyocarditis virus binds zinc, is phosphorylated during viral infection, and affects the efficiency of genome translation
Dvorak, C.M.T., Hall, D.J., Hill, M., Riddle, M., Pranter, A.
Virology (2001), 290, 261-271, PubMed 11883190 , DOI 10.1006/viro.2001.1193 ,
Related entities 1. zinc finger domain, : 1 : 2 : 13 entities Detail
Interaction partners 1. zinc finger domain, : 1 interactors Detail
Experiments performed 1 experiments Detail
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