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Solution structure of micelle-bound fusion domain of HIV-1 gp41
Authors
Jaroniec, C.P., Kaufman, J.D., Stahl, S.J., Viard, M., Blumenthal, R., Wingfield, P.T., Bax, A.
Assembly
Envelope polyprotein GP160
Entity
1. Envelope polyprotein GP160 (polymer, Thiol state: not present), 39 monomers, 3876.324 Da Detail

PAVGIGALFL GFLGAAGSTM GAASMTLTVQ ADYKDDDDK


Formula weight
3876.324 Da
Source organism
Human immunodeficiency virus 1
Exptl. method
NMR
Refine. method
simulated annealing molecular dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 59.8 %, Completeness (bb): 98.7 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All59.8 % (238 of 398)43.7 % (87 of 199)70.6 % (113 of 160)97.4 % (38 of 39)
Backbone98.7 % (229 of 232)97.6 % (81 of 83)99.1 % (110 of 111)100.0 % (38 of 38)
Sidechain21.1 % (42 of 199) 5.2 % (6 of 116)43.9 % (36 of 82) 0.0 % (0 of 1)
Aromatic 0.0 % (0 of 28) 0.0 % (0 of 14) 0.0 % (0 of 14)
Methyl25.0 % (12 of 48)12.5 % (3 of 24)37.5 % (9 of 24)

1. Envelope polyprotein GP160

PAVGIGALFL GFLGAAGSTM GAASMTLTVQ ADYKDDDDK

Sample

Pressure 1 atm, Temperature 298 K, pH 6.5


#NameIsotope labelingTypeConcentration
1Envelope polyprotein GP160[U-2H; U-13C; U-15N]0.7 mM
2sodium dodecyl sulfate75 mM
3sodium phosphate25 mM
4sodium azide0.05 % w/v
5H2O93 %
6D2O7 %

LACS Plot; CA
Referencing offset: 0.43 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: 0.43 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.01 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.58 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 30 models in PDB: 2ARI, Strand ID: A Detail


Release date
2005-12-27
Citation
Structure and dynamics of micelle-associated human immunodeficiency virus gp41 fusion domain
Jaroniec, C.P., Kaufman, J.D., Stahl, S.J., Viard, M., Blumenthal, R., Wingfield, P.T., Bax, A.
Biochemistry (2005), 44, 16167-16180, PubMed 16331977 , DOI 10.1021/bi051672a ,
Related entities 1. Envelope polyprotein GP160, : 1 : 2 entities Detail
Experiments performed 8 experiments Detail
nullKeywords domain, fusion, gp41, HIV, membrane, micelle, peptide, protein, virus