BMRB: 6868

Solution Structure of ydhR protein from Escherichia coli

Authors

Revington, M.J., Shaw, G.S.

Assembly

Protein ydhR precursor

Entity

1. Protein ydhR precursor (polymer, Thiol state: not present), 123 monomers, 13863.40 × 2 Da Detail

MGTSHHHHHH SSGRENLYFQ GHMATLLQLH FAFNGPFGDA MAEQLKPLAE SINQEPGFLW KVWTESEKNH EAGGIYLFTD EKSALAYLEK HTARLKNLGV EEVVAKVFDV NEPLSQINQA KLA

Total weight

27726.8 Da

Max. entity weight

13863.4 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

simulated annealing,torsion angle dynamics, molecular dynamics,

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 86.2 %, Completeness: 80.1 %, Completeness (bb): 84.0 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	80.1 % (1159 of 1447)	81.1 % (607 of 748)	78.1 % (441 of 565)	82.8 % (111 of 134)
Backbone	84.0 % (613 of 730)	84.9 % (213 of 251)	83.6 % (301 of 360)	83.2 % (99 of 119)
Sidechain	77.5 % (644 of 831)	79.3 % (394 of 497)	74.6 % (238 of 319)	80.0 % (12 of 15)
Aromatic	37.3 % (59 of 158)	40.5 % (32 of 79)	32.5 % (25 of 77)	100.0 % (2 of 2)
Methyl	96.8 % (122 of 126)	96.8 % (61 of 63)	96.8 % (61 of 63)

1. Protein ydhR precursor

MGTSHHHHHH SSGRENLYFQ GHMATLLQLH FAFNGPFGDA MAEQLKPLAE SINQEPGFLW KVWTESEKNH EAGGIYLFTD EKSALAYLEK HTARLKNLGV EEVVAKVFDV NEPLSQINQA KLA

Show sample condition

Sample

Pressure 1 atm, Temperature 303 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Protein ydhR precursor	[U-15N; U-13C]		0.5 mM
2	MES			25 mM
3	NaCl			450 mM
4	Sodium Azide			0.01 %
5	H2O			90 %
6	D2O			10 %

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 2ASY, Strand ID: A, B Detail

---

Release date

2006-04-16

Citation

Solution structure of the Escherichia coli protein ydhR: a putative mono-oxygenase
Revington, M., Semesi, A., Yee, A., Shaw, G.S.
Protein Sci. (2005), 14, 3115-3120, PubMed 16260765 , DOI 10.1110/ps.051809305 , Abstract

Related entities 1. Protein ydhR precursor, : 1 : 4 : 2 entities Detail

Interaction partners 1. Protein ydhR precursor, : 2 interactors Detail

Experiments performed 3 experiments Detail